LOCUS       sequence-237746-       11644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CD
FEATURES             Location/Qualifiers
     source          1..11644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..151
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        199..1377
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          430..1368
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1325..1345
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1397..1421
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4557..4581)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4634..4675
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4696..5263
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4713..5265
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4857..4874)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5084..5103
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5277..5279,5280..5282,5283..5996)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5277..5279,5280..5282,5283..5993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5322..5343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5583..5602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5930..5951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6010..6598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6063..6083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6481..6492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6673..6906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7025..7043)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7111..7215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7216..7284,7285..8076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7434..7453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8247..8835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8736..8755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8989..9006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9246..9267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9282..9287,9288..9305,9306..9312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9320..9336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9325..9347
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9344..9360
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9344..9360
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9410..9429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9621..10254
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10301..10426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10923..11156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11341..11385
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
       61 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      121 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      181 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      241 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      301 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      361 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      421 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      481 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
      541 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
      601 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
      661 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
      721 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
      781 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
      841 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
      901 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
      961 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     1021 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     1081 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     1141 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     1201 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     1261 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     1321 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc
     1381 ggccctgcag atatcaacaa gtttgtacaa aaaagttggc accatgcccc ctggggtgga
     1441 ctgccccatg gaattctgga ccaaggagga gaatcagagc gttgtggttg acttcctgct
     1501 gcccacaggg gtctacctga acttccctgt gtcccgcaat gccaacctca gcaccatcaa
     1561 gcagctgctg tggcaccgcg cccagtatga gccgctcttc cacatgctca gtggccccga
     1621 ggcctatgtg ttcacctgca tcaaccagac agcggagcag caagagctgg aggacgagca
     1681 acggcgtctg tgtgacgtgc agcccttcct gcccgtcctg cgcctggtgg cccgtgaggg
     1741 cgaccgcgtg aagaagctca tcaactcaca gatcagcctc ctcatcggca aaggcctcca
     1801 cgagtttgac tccttgtgcg acccagaagt gaacgacttt cgcgccaaga tgtgccaatt
     1861 ctgcgaggag gcggccgccc gccggcagca gctgggctgg gaggcctggc tgcagtacag
     1921 tttccccctg cagctggagc cctcggctca aacctggggg cctggtaccc tgcggctccc
     1981 gaaccgggcc cttctggtca acgttaagtt tgagggcagc gaggagagct tcaccttcca
     2041 ggtgtccacc aaggacgtgc cgctggcgct gatggcctgt gccctgcgga agaaggccac
     2101 agtgttccgg cagccgctgg tggagcagcc ggaagactac acgctgcagg tgaacggcag
     2161 gcatgagtac ctgtatggca gctacccgct ctgccagttc cagtacatct gcagctgcct
     2221 gcacagtggg ttgacccctc acctgaccat ggtccattcc tcctccatcc tcgccatgcg
     2281 ggatgagcag agcaaccctg ccccccaggt ccagaaaccg cgtgccaaac cacctcccat
     2341 tcctgcgaag aagccttcct ctgtgtccct gtggtccctg gagcagccgt tccgcatcga
     2401 gctcatccag ggcagcaaag tgaacgccga cgagcggatg aagctggtgg tgcaggccgg
     2461 gcttttccac ggcaacgaga tgctgtgcaa gacggtgtcc agctcggagg tgagcgtgtg
     2521 ctcggagccc gtgtggaagc agcggctgga gttcgacatc aacatctgcg acctgccccg
     2581 catggcccgt ctctgctttg cgctgtacgc cgtgatcgag aaagccaaga aggctcgctc
     2641 caccaagaag aagtccaaga aggcggactg ccccattgcc tgggccaacc tcatgctgtt
     2701 tgactacaag gaccagctta agaccgggga acgctgcctc tacatgtggc cctccgtccc
     2761 agatgagaag ggcgagctgc tgaaccccac gggcactgtg cgcagtaacc ccaacacgga
     2821 tagcgccgct gccctgctca tctgcctgcc cgaggtggcc ccgcaccccg tgtactaccc
     2881 cgccctggag aagatcttgg agctggggcg acacagcgag tgtgtgcatg tcaccgagga
     2941 ggagcagctg cagctgcggg aaatcctgga gcggcggggg tctggggagc tgtatgagca
     3001 cgagaaggac ctggtgtgga agctgcggca tgaagtccag gagcacttcc cggaggcgct
     3061 agcccggctg ctgctggtca ccaagtggaa caagcatgag gatgtggccc agatgctcta
     3121 cctgctgtgc tcctggccgg agctgcccgt cctgagcgcc ctggagctgc tagacttcag
     3181 cttccccgat tgccacgtag gctccttcgc catcaagtcg ctgcggaaac tgacggacga
     3241 tgagctgttc cagtacctgc tgcagctggt gcaggtgctc aagtacgagt cctacctgga
     3301 ctgcgagctg accaaattcc tgctggaccg ggccctggcc aaccgcaaga tcggccactt
     3361 ccttttctgg cacctccgct ccgagatgca cgtgccgtcg gtggccctgc gcttcggcct
     3421 catcctggag gcctactgca ggggcagcac ccaccacatg aaggtgctga tgaagcaggg
     3481 ggaagcactg agcaaactga aggccctgaa tgacttcgtc aagctgagct ctcagaagac
     3541 ccccaagccc cagaccaagg agctgatgca cttgtgcatg cggcaggagg cctacctaga
     3601 ggccctctcc cacctgcagt ccccactcga ccccagcacc ctgctggctg aagtctgcgt
     3661 ggagcagtgc accttcatgg actccaagat gaagcccctg tggatcatgt acagcaacga
     3721 ggaggcaggc agcggcggca gcgtgggcat catctttaag aacggggatg acctccggca
     3781 ggacatgctg accctgcaga tgatccagct catggacgtc ctgtggaagc aggaggggct
     3841 ggacctgagg atgaccccct atggctgcct ccccaccggg gaccgcacag gcctcattga
     3901 ggtggtactc cgttcagaca ccatcgccaa catccaactc aacaagagca acatggcagc
     3961 cacagccgcc ttcaacaagg atgccctgct caactggctg aagtccaaga acccggggga
     4021 ggccctggat cgagccattg aggagttcac cctctcctgt gctggctatt gtgtggccac
     4081 atatgtgctg ggcattggcg atcggcacag cgacaacatc atgatccgag agagtgggca
     4141 gctgttccac attgattttg gccactttct ggggaatttc aagaccaagt ttggaatcaa
     4201 ccgcgagcgt gtcccattca tcctcaccta cgactttgtc catgtgattc agcaggggaa
     4261 gactaataat agtgagaaat ttgaacggtt ccggggctac tgtgaaaggg cctacaccat
     4321 cctgcggcgc cacgggcttc tcttcctcca cctctttgcc ctgatgcggg cggcaggcct
     4381 gcctgagctc agctgctcca aagacatcca gtatctcaag gactccctgg cactggggaa
     4441 aacagaggag gaggcactga agcacttccg agtgaagttt aacgaagccc tccgtgagag
     4501 ctggaaaacc aaagtgaact ggctggccca caacgtgtcc aaagacaaca ggcagttgcc
     4561 aactttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg
     4621 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     4681 cggttagtaa tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4741 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4801 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4861 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4921 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4981 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5041 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5101 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     5161 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     5221 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatgg
     5281 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5341 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5401 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5461 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5521 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5581 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5641 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5701 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5761 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5821 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5881 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5941 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaatcg
     6001 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6061 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6601 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6661 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6721 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6781 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6841 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6901 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6961 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     7021 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7081 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7441 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     7501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9061 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9121 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9181 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9241 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9301 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9361 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9421 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9481 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9541 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9601 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     9661 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     9721 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     9781 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     9841 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     9901 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     9961 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
    10021 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
    10081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    10261 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    10321 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    10381 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    10441 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10501 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10561 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10621 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10681 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10741 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
    10801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    11041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    11101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    11161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11521 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11581 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    11641 tatc
//
