LOCUS       sequence-237751-       10924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DDR1__PAK1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DDR1__PAK1
FEATURES             Location/Qualifiers
     source          1..10924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    236..353
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        401..1579
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          632..1570
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1527..1547
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1599..1623
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2970..2994)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3052..3069)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4162..4182)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4236..4277
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4298..4865
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4315..4867
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4459..4476)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4686..4705
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4879..5478
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4879..4898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5375..5395
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5492..6080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5545..5565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5963..5974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6155..6388
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6507..6525)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6593..6697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6698..6766,6767..7558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6916..6935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7729..8317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8218..8237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8471..8488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8728..8749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8764..8769,8770..8787,8788..8794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8802..8818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8807..8829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8826..8842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8826..8842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8892..8911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9103..9736
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9783..9908
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10405..10638
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10823..10867
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
      241 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      301 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      361 attacaggga cagcagagat ccagtttgga ctagtcgtga ggctccggtg cccgtcagtg
      421 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      481 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      541 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      601 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      661 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      721 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      781 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      841 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      901 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      961 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1021 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1081 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1141 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1201 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1261 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1321 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1381 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1441 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1501 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1561 tccatttcag gtgtcgtgaa gcggccctgc agatatcaac aagtttgtac aaaaaagttg
     1621 gcatgggacc agaggccctg tcatctttac tgctgctgct cttggtggca agtggagatg
     1681 ctgacatgaa gggacatttt gatcctgcca agtgccgcta tgccctgggc atgcaggacc
     1741 ggaccatccc agacagtgac atctctgctt ccagctcctg gtcagattcc actgccgccc
     1801 gccacagcag gttggagagc agtgacgggg atggggcctg gtgccccgca gggtcggtgt
     1861 ttcccaagga ggaggagtac ttgcaggtgg atctacaacg actgcacctg gtggctctgg
     1921 tgggcaccca gggacggcat gccgggggcc tgggcaagga gttctcccgg agctaccggc
     1981 tgcgttactc ccgggatggt cgccgctgga tgggctggaa ggaccgctgg ggtcaggagg
     2041 tgatctcagg caatgaggac cctgagggag tggtgctgaa ggaccttggg ccccccatgg
     2101 ttgcccgact ggttcgcttc tacccccggg ctgaccgggt catgagcgtc tgtctgcggg
     2161 tagagctcta tggctgcctc tggagggatg gactcctgtc ttacactgcc cctgtggggc
     2221 agacaatgta tttatctgag gccgtgtacc tcaacgactc cacctatgac ggacataccg
     2281 tgggcggact gcagtatggg ggtctgggcc agctggcaga tggtgtggtg gggctggatg
     2341 actttaggaa gagtcaggag ctgcgggtct ggccaggcta tgactatgtg ggatggagca
     2401 accacagctt ctccagtggc tatgtggaga tggagtttga gtttgaccgg ctgagggcct
     2461 tccaggctat gcaggtccac tgtaacaaca tgcacacgct gggagcccgt ctgcctggcg
     2521 gggtggaatg tcgcttccgg cgtggccctg ccatggcctg ggagggggag cccatgcgcc
     2581 acaacctagg gggcaacctg ggggacccca gagcccgggc tgtctcagtg ccccttggcg
     2641 gccgtgtggc tcgctttctg cagtgccgct tcctctttgc ggggccctgg ttactcttca
     2701 gcgaaatctc cttcatctct gatgtggtga acaattcctc tccggcactg ggaggcacct
     2761 tcccgccagc cccctggtgg ccgcctggcc cacctcccac caacttcagc agcttggagc
     2821 tggagcccag aggccagcag cccgtggcca aggccgaggg gagcccgacc gccatcctca
     2881 tcggctgcct ggtggccatc atcctgctcc tgctgctcat cattgccctc atgctctggc
     2941 ggctgcactg gcgcaggctc ctcagcaagt cccagctttc ttgtacaaag tggttcaatg
     3001 tgaaggctgt gtctgagact cctgcagtgc caccagtttc agaagatgag gatgatgatg
     3061 atgatgatgc taccccacca ccagtgattg ctccacgccc agagcacaca aaatctgtat
     3121 acacacggtc tgtgattgaa ccacttcctg tcactccaac tcgggacgtg gctacatctc
     3181 ccatttcacc tactgaaaat aacaccactc caccagatgc tttgacccgg aatactgaga
     3241 agcagaagaa gaagcctaaa atgtctgatg aggagatctt ggagaaatta cgaagcatag
     3301 tgagtgtggg cgatcctaag aagaaatata cacggtttga gaagattgga caaggtgctt
     3361 caggcaccgt gtacacagca atggatgtgg ccacaggaca ggaggtggcc attaagcaga
     3421 tgaatcttca gcagcagccc aagaaagagc tgattattaa tgagatcctg gtcatgaggg
     3481 aaaacaagaa cccaaacatt gtgaattact tggacagtta cctcgtggga gatgagctgt
     3541 gggttgttat ggaatacttg gctggaggct ccttgacaga tgtggtgaca gaaacttgca
     3601 tggatgaagg ccaaattgca gctgtgtgcc gtgagtgtct gcaggctctg gagttcttgc
     3661 attcgaacca ggtcattcac agagacatca agagtgacaa tattctgttg ggaatggatg
     3721 gctctgtcaa gctaactgac tttggattct gtgcacagat aaccccagag cagagcaaac
     3781 ggagcaccat ggtaggaacc ccatactgga tggcaccaga ggttgtgaca cgaaaggcct
     3841 atgggcccaa ggttgacatc tggtccctgg gcatcatggc catcgaaatg attgaagggg
     3901 agcctccata cctcaatgaa aaccctctga gagccttgta cctcattgcc accaatggga
     3961 ccccagaact tcagaaccca gagaagctgt cagctatctt ccgggacttt ctgaaccgct
     4021 gtctcgagat ggatgtggag aagagaggtt cagctaaaga gctgctacag catcaattcc
     4081 tgaagattgc caagcccctc tccagcctca ctccactgat tgctgcagct aaggaggcaa
     4141 caaagaacaa tcactaacac ccaacttttc tatacaaagt ggttgatatc cagcacagtg
     4201 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     4261 tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta
     4321 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4381 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4441 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4501 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4561 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4621 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4681 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4741 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4801 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4861 gataatatgg ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4921 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4981 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5041 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5101 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5161 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5221 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5281 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5341 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5401 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5461 cgcaagcccg gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga
     5521 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5581 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5641 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5701 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5761 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5821 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5881 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5941 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6001 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6061 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     6121 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6181 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6241 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6301 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6361 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6421 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     6481 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6541 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6601 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6661 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6721 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6781 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6841 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6901 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6961 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7021 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7081 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7141 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7201 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7261 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7321 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7381 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7441 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7501 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7561 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7621 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7861 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8401 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8461 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8521 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     8581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8641 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     8701 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8761 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8821 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     8881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     8941 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9001 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9061 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     9121 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     9181 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     9241 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     9301 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     9361 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     9421 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     9481 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     9541 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9721 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9781 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9841 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9901 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9961 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    10021 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    10081 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    10141 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    10201 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
    10261 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10321 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    10381 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    10441 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    10501 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    10561 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    10621 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    10681 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10741 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10801 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10861 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10921 gata
//
