LOCUS       sequence-237755-        9155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SRSF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-SRSF2
FEATURES             Location/Qualifiers
     source          1..9155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    103..220
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        268..1446
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          499..1437
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1394..1414
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1466..1490
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2160..2184)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2192..2210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2235..2254)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2236..2254)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2260..2280)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2334..2375
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2396..2963
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2413..2965
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2557..2574)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2784..2803
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2977..3576
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2977..2996)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3473..3493
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3590..4178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3643..3663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4061..4072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4253..4486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4605..4623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4691..4795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4796..4864,4865..5656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5014..5033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5827..6415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6316..6335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6569..6586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6826..6847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6862..6867,6868..6885,6886..6892)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6900..6916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6905..6927
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6924..6940
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6924..6940
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6990..7009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7201..7834
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7881..8006
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8503..8736
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8921..8965
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
       61 gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      121 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      181 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      241 cagagatcca gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
      301 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      361 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      421 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      481 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      541 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      601 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      661 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      721 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      781 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      841 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      901 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      961 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     1021 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     1081 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1141 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1201 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1261 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1321 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1381 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1441 tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa aaagcaggca ccatgagcta
     1501 cggccgcccc cctcccgatg tggagggtat gacctccctc aaggtggaca acctgaccta
     1561 ccgcacctcg cccgacacgc tgaggcgcgt cttcgagaag tacgggcgcg tcggcgacgt
     1621 gtacatcccg cgggatcgct acaccaagga gtcccgcggc ttcgccttcg ttcgctttca
     1681 cgacaagcgc gacgctgagg acgctatgga tgccatggac ggggccgtgc tggacggccg
     1741 cgagctgcgg gtgcaaatgg cgcgctacgg ccgccccccg gactcacacc acagccgccg
     1801 gggaccgcca ccccgcaggt acgggggcgg tggctacgga cgccggagcc gcagccctag
     1861 gcggcgtcgc cgcagccgat cccggagtcg gagccgttcc aggtctcgca gccgatctcg
     1921 ctacagccgc tcgaagtctc ggtcccgcac tcgttctcga tctcggtcga cctccaagtc
     1981 cagatccgca cgaaggtcca agtccaagtc ctcgtcggtc tccagatctc gttcgcggtc
     2041 caggtcccgg tctcggtcca ggagtcctcc cccagtgtcc aagagggaat ccaaatccag
     2101 gtcgcgatcg aagagtcccc ccaagtctcc tgaagaggaa ggagcggtgt cctcttagaa
     2161 cccagctttc ttgtacaaag tggtgatatc caattaaccc tcactaaagg aatgtaaagt
     2221 aaagaaagtg atgaccctat agtgagtcgt attaccaccc aacttttcta tacaaagtgg
     2281 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc
     2341 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagatccct
     2401 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     2461 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     2521 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     2581 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     2641 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     2701 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     2761 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     2821 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     2881 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2941 tcctttgaaa aacacgatga taatatggcc acacatatga ccgagtacaa gcccacggtg
     3001 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     3061 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     3121 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3181 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3241 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3301 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3361 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3421 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     3481 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     3541 cgcacctggt gcatgacccg caagcccggt gcctaaatcg atagatccta atcaacctct
     3601 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3661 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3721 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3781 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3841 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3901 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3961 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4021 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4081 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4141 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
     4201 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4261 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     4501 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
     4561 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
     4621 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     5041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6541 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6601 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
     6661 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6721 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
     6781 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6841 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6901 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
     6961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7021 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     7081 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     7141 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     7201 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     7261 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     7321 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     7381 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     7441 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     7501 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     7561 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     7621 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     7681 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7741 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7801 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7861 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7921 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7981 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8041 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8101 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8161 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8221 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8281 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8341 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8401 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8461 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8521 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8581 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8641 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8701 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8761 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8821 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8881 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8941 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9001 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9061 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9121 cccaaccccg aggggacccg acaggcccga aggaa
//
