LOCUS       sequence-237759-        8257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA 
            (plasmid 122227). With five silent mutations at sh1 shRNA target 
            site. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali
            R, Chen WV, Maniatis T, De Blas AL
  TITLE     Molecular and functional interaction between protocadherin-gammaC5 
            and GABAA receptors.
  JOURNAL   J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 
            10.1523/JNEUROSCI.0969-12.2012.
  PUBMED    22915120
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0969-12"; journalName: "J Neurosci"; date: 
            "2012-08-22- 22"; volume: "32"; issue: "34"; pages: "11780-97"
COMMENT     Sequence Label: Rescue myc-PCDHGC5 mRNA
FEATURES             Location/Qualifiers
     source          1..8257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1306..1335
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3848..3865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3854..4078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4124..4552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4211..4230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4421..4442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4561..4581)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4566..4895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4746..4881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4808..4827
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4962..5756
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5016..5035)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5626..5645
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5930..6051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5967..5986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6021..6040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6100..6116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6100..6116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6113..6135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6124..6140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6148..6154,6155..6172,6173..6178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6193..6214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6331..6348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6502..7090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6582..6601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7261..8052,8053..8121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7884..7903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8122..8226)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 tagaccgcca tggggcctat ggcatcacca caggtcactg gtaaacggca agtgctgtat
      961 atgttgtcct tgtgctgctg tggctgggtg tctgggcagc ttcgttattc agtggtggag
     1021 gaatcagagc cggggacttt ggtggggaac gttgctcaag atctgggctt aaaggtggca
     1081 gatctgttga gtcgcaggct gagactgggc tctgaggaga acgggcgcta tttttccctg
     1141 agcttgatga gtggagcttt ggcagtgaat ctaaaaattg accgagagag cctgtgtgga
     1201 gccagcacga gctgcctgct gcctgtacag gtggtaaccg aacaccccct ggagctcacc
     1261 cgggtagagg tagagatcct agatctcaac gacaactctc ctagcgaaca aaaactaata
     1321 tcagaagaag acctatttgc cactcctgag cgagagatgc gcatctcaga atcagcggca
     1381 cctggagcca ggttcccact ggacagtgcc caagacccag acgtgggcac caatactgtg
     1441 agcttttata ctctaagccc cagcagtcac ttctctctga atgtgaagac cctaaaagat
     1501 gggaagctct tcccagagct agtgctagag cagcagctag accgggaagc ccaggcaagg
     1561 catcagctgg tactcactgc tgtggatggg ggaaccccag cccgctcagg gaccagcctt
     1621 atctctgtca ttgtgctgga catcaatgat aatgccccaa ccttccagtc ctcagttcta
     1681 cgtgtgggac tcccagagaa cacacccccg gggaccctgc tgctccgtct caatgccact
     1741 gatccagacg agggcaccaa tggccaacta gactactctt tcggagacca tacgtccgag
     1801 gctgttaaga atctctttgg cctggatcct aacactggag caatccacgt attgggtccg
     1861 gtagattttg aggaatcaaa tttctatgaa attcatgcaa gagctcgaga ccagggacag
     1921 ccagccatgg agggccactg tgtaatccag gtggatgtag gggatgctaa cgacaatccc
     1981 cctgaggtgc tgctggcatc tttggtcaac cctgtcctag agagcacacc cgtgggcaca
     2041 gttgtggggt tgtttaatgt gcgggaccga gactcaggta gaaatggtga ggtgatcctg
     2101 gagacaactc caaacctgcc atttcagatc aagccttccg agaaccatta ctcactgctc
     2161 accagccagc ctttggaccg agaggtcaca tcccattaca ccatcgacct gctggccagt
     2221 gatgctggat cacctcccct gcatacacat ctcaccatca ggctgaacat ttcagatgtt
     2281 aatgacaacg caccccattt cacccaacag ctctacactg cttatatccc agaaaaccgg
     2341 ccaccaggct ctctcctctg tactgtggct gcctctgatc cagatgaggg ggataatgcc
     2401 cgcctcacct attctattgt gggaagtcaa attcagggag ccccagcctc ctccttcgtg
     2461 tatgtcaacc ccgaagatgg acgagtcttt gcccagcgga cctttgacta tgaattactg
     2521 cagatgctgc agattgtggt gggggtccga gattccggct ctccccgact gtatgctaac
     2581 acatctctcc atgtgtttgt ccttgatcag aacgataacg ccccaactgt actgcaccca
     2641 cgatctggca gggagttctc agcccctcag cgtctccccc gatctgctcc tcctggttct
     2701 ctggtcacca aggtgacagc tgtggatgct gatgctggtc acaatgcatg gctctcttac
     2761 tctctattac ctcagtccac agcccctgga ctgttcctcg tatctacaca cactggtgag
     2821 gtgcgcacag cccgggcctt attggaggac gactctgata cccaacaggt ggtggtcctg
     2881 gtgagggaca atggtgaccc ttcactatcc tccacagcaa cagtattgct ggttctggag
     2941 gacgaggatg cagaggaaat gcccaaatcc agcgacttcc tcacacaccc tcccgagcgg
     3001 tcagacctca ccctttatct tattgtggcg ctggcagctg tcagtctctt atccctagtt
     3061 accttcactt tcatgtcagc taagtgtctg cggaggcatg aagataggga ccggggtggg
     3121 ggccagtgct gcaggggcca ggactcgccc tccagggagt tctataaaca gtccagtccc
     3181 aaccttcagg tcagctcaga tggcactcta aagtacatgg aggtgaccct gcggcccaca
     3241 gattctcaga gccattgcta caggacgtgc ttttctccag cctccgatgg cagtgacttc
     3301 actttcctca ggcccctcag cgttcagcag ccctcagcct tggcgctgga gcccgaggcc
     3361 ttgcgttccc ggtctagtac gctgcgggag cggagccagc aagccccgcc caacactgac
     3421 tggcgtttct ctcaagccca gagacccggc acgagcggat cccaaaatgg ggatgaaacc
     3481 ggcacctggc ccaacaacca gttcgataca gagatgctgc aagccatgat cttggcctct
     3541 gccagtgaag ccgctgatgg gagctccact ctgggagggg gcactggcac tatgggtttg
     3601 agtgctcgat atggacccca gtttaccctg cagcacgtgc ctgactaccg ccagaacgtg
     3661 tacatccctg gcagcaatgc cacactgacc aacgcagctg gcaaacgaga tggcaaggct
     3721 ccggcaggcg gcaatggcaa caagaagaag tcgggcaaga aagagaagaa gtaaccggaa
     3781 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     3841 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3901 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3961 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4021 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4081 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4141 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4201 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4261 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4321 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4381 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4441 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4501 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4561 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4621 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4801 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     4861 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     4921 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     4981 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5041 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5101 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5161 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5221 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5281 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5341 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5401 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5461 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5521 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5581 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5641 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5701 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5761 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5821 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5881 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     5941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6061 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6661 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6721 ttcggtgtag gtcgttcgcc ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6901 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8221 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
