LOCUS       sequence-237767-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00115881
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(326..342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(326..342)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(339..361)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    350..366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(374..380,381..398,399..404))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(557..574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acttggtctc taatgttgaa tattgttgtt ggtcatatta ctactggtaa acaatgttgt
       61 gatcatggtg ctgctgatga atctagaatt tgtgaaagaa aaggtttgaa tgcttattgt
      121 tgttctgatg ctagaaatga tgctaaaggt ggttgtaaag aagaagttta tgaagttggt
      181 agattggttc aagctttttc tactaatggt atttgttctg gtcaatcttc ttctggtgat
      241 actttgcaag gttttgttgg ttgtgctgaa tgagcttaga gaccaagttc catcccaatg
      301 gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      361 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      421 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      481 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      541 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      781 agaggtggcg aaacccgaca ggactataaa gatacc
//
