LOCUS       sequence-237769-       12142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses disordered protein HP1-alpha fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-HP1-alpha-miRFP670-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             677..1621
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             1634..3127
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3279..3867
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3332..3352)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3750..3761)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3870..3886)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3871..3887)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3977..4210
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4235..4253)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4236..4253)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4543..4562)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4776..4795)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4895..4917
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4955..4973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5041..5145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5146..5214,5215..6006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5364..5383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6177..6765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6666..6685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6919..6936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7006..7026)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7011..7340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7191..7326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8474..8539
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8669..8689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9114..9248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9151..9170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9205..9224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9416..10049
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10096..10221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10718..10951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11136..11180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11465..11582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11735..12142
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatgggaaa
       61 gaaaaccaag cggacagctg acagttcttc ttcagaggat gaggaggagt atgtcgtgga
      121 gaaggtgcta gacaggcgcg tggttaaggg acaagtggaa tatctactga agtggaaagg
      181 cttttctgag gagcacaata cttgggaacc tgagaaaaac ttggattgcc ctgagctaat
      241 ttctgaattt atgaaaaagt ataagaagat gaaggagggt gaaaataata aacccaggga
      301 gaagtcagaa agtaacaaga ggaaatccaa tttctcaaac agtgccgatg acatcaaatc
      361 taaaaaaaag agagagcaga gcaatgatat cgctcggggc tttgagagag gactggaacc
      421 agaaaagatc attggggcaa cagattcctg tggtgattta atgttcctaa tgaaatggaa
      481 agacacagat gaagctgacc tggttcttgc aaaagaagct aatgtgaaat gtccacaaat
      541 tgtgatagca ttttatgaag agagactgac atggcatgca tatcctgagg atgcggaaaa
      601 caaagagaaa gaaacagcaa agagcggaat tctgcagtcg acggtaccgc gggcccggga
      661 tccaccggtc gccaccatgg tagcaggtca tgcctctggc agccccgcat tcgggaccgc
      721 ctctcattcg aattgcgaac atgaagagat ccacctcgcc ggctcgatcc agccgcatgg
      781 cgcgcttctg gtcgtcagcg aacatgatca tcgcgtcatc caggccagcg ccaacgccgc
      841 ggaatttctg aatctcggaa gcgtactcgg cgttccgctc gccgagatcg acggcgatct
      901 gttgatcaag atcctgccgc atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg
      961 ctgccggatc ggcaatccct ctacggagta ctgcggtctg atgcatcggc ctccggaagg
     1021 cgggctgatc atcgaactcg aacgtgccgg cccgtcgatc gatctgtcag gcacgctggc
     1081 gccggcgctg gagcggatcc gcacggcggg ttcactgcgc gcgctgtgcg atgacaccgt
     1141 gctgctgttt cagcagtgca ccggctacga ccgggtgatg gtgtatcgtt tcgatgagca
     1201 aggccacggc ctggtattct ccgagtgcca tgtgcctggg ctcgaatcct atttcggcaa
     1261 ccgctatccg tcgtcgactg tcccgcagat ggcgcggcag ctgtacgtgc ggcagcgcgt
     1321 ccgcgtgctg gtcgacgtca cctatcagcc ggtgccgctg gagccgcggc tgtcgccgct
     1381 gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgtgccatct
     1441 gcagttcctg aaggacatgg gcgtgcgcgc caccctggcg gtgtcgctgg tggtcggcgg
     1501 caagctgtgg ggcctggttg tctgtcacca ttatctgccg cgcttcatcc gtttcgagct
     1561 gcgggcgatc tgcaaacggc tcgccgaaag gatcgcgacg cggatcaccg cgcttgagag
     1621 cgcggccacg cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag
     1681 gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt
     1741 catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat
     1801 gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt
     1861 aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac
     1921 aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa
     1981 ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga
     2041 accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa
     2101 gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc
     2161 aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa
     2221 tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa
     2281 tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag
     2341 caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat
     2401 aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa
     2461 gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta
     2521 ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct
     2581 tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac
     2641 cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa
     2701 cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg
     2761 gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg
     2821 ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc
     2881 cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga
     2941 gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact
     3001 caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac
     3061 agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg
     3121 agcagcagcc cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag
     3181 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     3241 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     3301 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3361 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3421 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3481 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3541 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3601 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3661 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3721 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3781 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3841 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     3901 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3961 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4021 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4201 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     4261 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     4321 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     4381 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     4441 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     4501 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     4561 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     4621 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     4681 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     4741 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4801 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4861 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4921 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4981 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     7021 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7081 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7141 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7201 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7261 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7321 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     7381 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     7441 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     7501 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     7561 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     7621 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     7681 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     7741 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     7801 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     7861 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     7921 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     7981 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     8041 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     8101 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     8161 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     8221 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     8281 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     8341 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     8401 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     8461 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     8521 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     8581 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     8641 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     8701 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     8761 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     8821 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     8881 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     8941 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     9001 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9061 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9241 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9301 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     9361 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     9421 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     9481 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     9541 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     9601 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     9661 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     9721 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     9781 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     9841 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     9901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    10081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    10501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    10561 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    10621 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10681 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10741 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10801 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10861 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    10921 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    10981 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11041 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11101 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11161 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11221 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    11281 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    11341 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    11401 acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc
    11461 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    11521 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    11581 ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat
    11641 tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
    11701 agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa
    11761 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
    11821 gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
    11881 gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg
    11941 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
    12001 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
    12061 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
    12121 ccgacagact gagtcgcccg gg
//
