LOCUS       sequence-237774-        8432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed barcode for sample multiplexing in single-cell analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Guo C, Kong W, Kamimoto K, Rivera-Gonzalez GC, Yang X, Kirita Y, 
            Morris SA
  TITLE     CellTag Indexing: genetic barcode-based sample multiplexing for 
            single-cell genomics.
  JOURNAL   Genome Biol. 2019 May 9;20(1):90. doi: 10.1186/s13059-019-1699-y.
  PUBMED    31072405
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-05-9"; volume: "20(1):90. doi"; pages: " 
            10.1186/s13059-019-1699-y"
COMMENT     Sequence Label: CellTagA
FEATURES             Location/Qualifiers
     source          1..8432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             532..712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    759..884
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1377..1610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1795..1839
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2106..2223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2355..2489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2540..3253,3254..3256,3257..3259))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2543..3253,3254..3256,3257..3259))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2585..2606)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2934..2953
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3193..3214
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3363..3401)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    complement(3835..3868)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3883..4007
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4032..4037,4038..4055,4056..4062)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(4080..4096)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4080..4096)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(4097..4130)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4136..4724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4189..4209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4607..4618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4811..5044
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5116..5237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5277..5412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5432..5451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5433..5451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5461..5478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5461..5477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5470..5492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5619..6074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5706..5725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5916..5937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6100..6204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6205..6273,6274..7065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6423..6442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7236..7824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7725..7744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7978..7995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8112..8133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8148..8153,8154..8171,8172..8178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8186..8202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8191..8213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8210..8226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8210..8226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8245..8265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8247..8265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        join(8366..8432,1..313)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
       61 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      121 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      181 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      241 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      301 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      361 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      421 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      481 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc ggggtctctc
      541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      721 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      781 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      841 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      901 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      961 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1021 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1081 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1141 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1201 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1261 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1321 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1381 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1441 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1501 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1561 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1621 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1681 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1741 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1801 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1861 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1921 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1981 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2041 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     2101 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2161 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2221 tttatcgatc acgagactag cctcgagtga tcataatcag ccataccaca tttgtagagg
     2281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2341 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa ggcaatagca
     2401 tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt ttgtccaaac
     2461 tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg cctgtcatcg
     2521 aattcatata gcaaccggtt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     2581 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     2641 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     2701 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     2761 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     2821 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     2881 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     2941 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     3001 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     3061 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     3121 acttcagggt cagcttaccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     3181 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     3241 cctcgccctt gctcaccatg gtggtaccga attccgcgga ggctggatcg gtcccggtgt
     3301 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
     3361 cgagctctgc ttatataggc ctcccaccgt acacgcctac cactagtaat gaaagacccc
     3421 acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg gaaaaatacc
     3481 aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct aacgttgggc
     3541 caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga acagatggtc
     3601 accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata tggcccaacc
     3661 ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac ctgaaatgac
     3721 cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg cgcgcttctg
     3781 cttcccgagc tctataaaag agctcacaac ccctcactcg gcttaattaa gatcataact
     3841 tcgtatagca tacattatac gaagttatcc cagcttgata tcacaagttt gtacaaaaaa
     3901 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     3961 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     4021 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     4081 atccactagt tctagaataa cttcgtatag catacattat acgaagttat tcgacaatca
     4141 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4201 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4261 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4321 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4381 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4441 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4501 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4561 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4621 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4681 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg
     4741 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4801 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5041 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5101 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5221 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5281 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5341 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5401 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     5461 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5521 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5581 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     5641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5761 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5821 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5941 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6061 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6181 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6241 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6421 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6721 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7081 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7141 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7381 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7861 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7921 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7981 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8041 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8101 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8161 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8221 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     8281 ctgcaagctt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     8341 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     8401 attacggggt cattagttca tagcccatat at
//
