LOCUS       sequence-237777-        5878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression construct for the production and purification 
            of Arc missing C-terminal Domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Pastuzyn ED, Day CE, Kearns RB, Kyrke-Smith M, Taibi AV, McCormick 
            J, Yoder N, Belnap DM, Erlendsson S, Morado DR, Briggs JAG, 
            Feschotte C, Shepherd JD
  TITLE     The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag 
            Protein that Mediates Intercellular RNA Transfer.
  JOURNAL   Cell. 2018 Mar 22;173(1):275. doi: 10.1016/j.cell.2018.03.024.
  PUBMED    29570995
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2018-03-22"; volume: "173(1):275. doi"; pages: " 
            10.1016/j.cell.2018.03.024"
COMMENT     Sequence Label: pGEX-6p1-GST-Arc-Delta-CTD
FEATURES             Location/Qualifiers
     source          1..5878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..684
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1671..1693)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1838..1856)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1924..2028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2029..2097,2098..2889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2247..2266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3060..3648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3549..3568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3802..3819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3892..3969
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3970..5052
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3989..4008)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5101..5106,5107..5124,5125..5131)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5139..5155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5163..5179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5163..5179
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5175..5702
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(5195..5212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5195..5211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5204..5226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(5804..5809,5810..5825,5826..5832)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5840..5856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggaagttc tgttccaggg gcccctggga tccatggagc tggaccatat gacgaccggc
      721 ggcctccacg cctaccctgc cccgcggggt gggccggccg ccaaacccaa tgtgatcctg
      781 cagattggta agtgccgagc tgagatgctg gagcacgtac ggaggaccca ccggcatctg
      841 ttgaccgaag tgtccaagca ggtggagcga gagctgaaag ggttgcacag gtcggtgggc
      901 aagctggaga acaacttgga cggctatgtg cccacgggcg actcacagcg ctggaagaag
      961 tccatcaagg cctgtctctg ccgctgccag gagaccatcg ccaacctgga gcgctgggtc
     1021 aagcgtgaga tgcacgtgtg gagggaggtc ttctaccgtc tggagaggtg ggccgaccgc
     1081 ctggagtcca tgggcggcaa gtacccagtg ggcagcgagc cggcccgcca cactgtctct
     1141 gtaggtgtgg ggggtccaga gccctactgc caggaagctg atggctacga ctacactgtt
     1201 agcccctatg ccatcacccc gccacctgcc gcaggagagc tgcctgagca ggagtcagtt
     1261 ggggctcagc aataccagtc ttgggtgcca ggtgaggatg ggcaaccaag cccaggtgtg
     1321 gatacccaga tctttgagga cccacgggag ttcctgagcc acctggaaga gtacctgcgg
     1381 caggtgggtg gctctgaaga atattggctg tcccagatcc agaaccacat gaatgggcca
     1441 gccaagaagt ggtgggagtt caaacagggc tcggtgaaga actgggtgga gttcaagaag
     1501 gagtttctgc agtacagtga gggtcccaca gaggatgaga ctgaggcact cacgcctgct
     1561 cttaccagcg agtcagtagc cagtgacagg acccagcctg aatagctcga gcggccgcat
     1621 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1681 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1741 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1801 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     1861 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1921 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1981 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2041 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2101 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2161 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2221 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     2281 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3841 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc
     3901 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     3961 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     4021 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     4081 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     4141 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     4201 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     4261 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     4321 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     4381 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     4441 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     4501 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     4561 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     4621 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     4681 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     4741 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     4801 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     4861 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     4921 ctgttgcccg tctcactggt gaaaagaaaa accacgctag cgcccaatac gcaaaccgcc
     4981 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5041 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccccaggc
     5101 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5161 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
     5221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5341 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     5401 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     5461 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc
     5521 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     5581 aggaaggcca gacgcgaatt atttttgatg gcgttggaat tagcttatcg actgcacggt
     5641 gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt
     5701 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
     5761 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
     5821 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagtattc
//
