LOCUS       sequence-237778-       11028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-tagged human PIKfyve in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Baranov MV, Bianchi F, Schirmacher A, van Aart MAC, Maassen S, 
            Muntjewerff EM, Dingjan I, Ter Beest M, Verdoes M, Keyser SGL, 
            Bertozzi CR, Diederichsen U, van den Bogaart G
  TITLE     The Phosphoinositide Kinase PIKfyve Promotes Cathepsin-S-Mediated 
            Major Histocompatibility Complex Class II Antigen Presentation.
  JOURNAL   iScience. 2019 Jan 25;11:160-177. doi: 10.1016/j.isci.2018.12.015. 
            Epub 2018 Dec 20.
  PUBMED    30612035
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:160-177. doi"; pages: " 
            10.1016/j.isci.2018.12.015. Epub 2018 Dec 20"
COMMENT     Sequence Label: GFP-hPIKfyve
FEATURES             Location/Qualifiers
     source          1..11028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1310..1319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1316..1318,1319..1321,1322..2032)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1316..1318,1319..1321,1322..2032)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1361..1382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1622..1641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1969..1990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4775..4786
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8519..8640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8556..8575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8610..8629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8647..9102)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8784..8805)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8996..9015
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9129..9233
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9235..9592
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9235..9426
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9258..9278)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9443..9578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9627..10421
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9627..10421
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9681..9700)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10291..10310
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10609..10628)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10653..10700
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt
     1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1501 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1801 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1921 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     2041 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cggtcaccat
     2101 ggccacagat gataagacgt ccccaacact ggactctgct aatgatttgc ctcgatctcc
     2161 tactagtcct tctcatctca cacactttaa acctttgact cctgatcaag atgagccccc
     2221 ttttaaatca gcttatagtt cttttgtaaa tctctttcgt tttaacaaag agagagcaga
     2281 aggaggccag ggagaacagc agcctttgag tggaagttgg accagccctc agctcccttc
     2341 gaggacacag tctgttaggt cacccacacc ttataaaaag cagcttaatg aggaactcca
     2401 gcggcgctct tcagcattag acacaagaag gaaagcagaa cctacctttg gaggtcatga
     2461 ccctcgtaca gctgttcagc ttcgaagcct cagcacagta ttaaaacgcc tcaaggaaat
     2521 catggagggg aaaagccagg atagtgacct gaaacaatac tggatgccag atagccaatg
     2581 taaagagtgc tatgactgta gtgagaaatt tacaaccttt aggcgcagac accattgccg
     2641 actatgtggg cagattttct gcagtcgttg ctgtaatcaa gaaatccctg gaaaatttat
     2701 gggctataca ggagacctcc gagcttgcac atattgtaga aaaatagcct taagttatgc
     2761 tcattccaca gacagtaatt ctattgggga agacttgaat gctctttcag attctgcttg
     2821 ctctgtgtct gtgcttgatc caagtgaacc ccgaacacct gttgggagta ggaaagccag
     2881 ccgtaacata tttttagagg atgatttggc ctggcaaagt ttgattcatc cagattcctc
     2941 aaatactcct ctttcaacaa gacttgtatc tgtgcaagag gatgctggga aatctcctgc
     3001 tcgaaataga tcagccagca ttactaacct gtcactggat agatctggtt ctcctatggt
     3061 accttcatat gagacatctg tcagtcccca ggctaaccga acatatgtta ggacagagac
     3121 cactgaggat gaacgcaaaa ttcttctgga cagtgtgcag ttaaaagacc tgtggaaaaa
     3181 aatctgccat cacagcagtg gaatggagtt tcaggatcac cgctactggt tgagaacgca
     3241 tcccaactgc attgtaggaa aggaattagt caactggcta atccgaaatg ggcatattgc
     3301 cacaagggca caagctatag caattggaca agcaatggtt gatggacgtt ggctggattg
     3361 tgttagtcat cacgaccagc ttttcagaga tgagtatgcg ctgtatagac cactgcagag
     3421 tacagaattt tctgagacgc cttctcccga cagtgactca gtgaactccg tggaaggaca
     3481 ctctgagcca tcctggttta aagacataaa gtttgatgac agtgacacag aacagatagc
     3541 tgaagaaggt gacgataatt tggctaattc tgccagtcct agcaagcgca catcagtcag
     3601 cagtttccag tccacagtgg acagtgactc agccgcttct atcagcctga acgtggagct
     3661 ggacaacgtg aacttccata tcaagaagcc ctccaagtac ccacatgtgc cccctcaccc
     3721 tgctgaccaa aaagagtatt tgatttctga cactggagga caacagctct caataagtga
     3781 cgctttcatc aaagaatcct tatttaatcg ccgagtagag gaaaaatcca aagagctgcc
     3841 tttcacacct ttgggctggc atcataacaa cctggagctc ctgagggagg agaatgggga
     3901 gaaacaagcc atggagaggt tgctttcagc taatcataac cacatgatgg cactactcca
     3961 gcagttgctc catagtgact cactgtcatc atcttggagg gacatcatcg tgtcattggt
     4021 ctgccaggtt gttcagacag tccgacctga tgtcaagaac caggatgatg acatggatat
     4081 ccgtcagttt gtccacatca aaaaaatccc aggtggaaag aagtttgatt ctgtggttgt
     4141 caatggcttt gtttgtacca agaacattgc acataaaaag atgagttctt gtattaaaaa
     4201 ccccaaaatt cttctgttga agtgttccat tgagtatctc tacagagaag aaactaagtt
     4261 tacttgcatt gatcctattg tgcttcagga aagggaattc ttgaagaatt atgtccagcg
     4321 aatagttgat gttcgaccca ccttggttct tgttgagaaa acagtgtctc ggattgccca
     4381 ggacatgtta ttggaacatg gcattacttt ggtcattaat gtaaagtcac aagttttgga
     4441 acgaatcagt cgaatgaccc aaggtgattt agtgatgtca atggaccagc tgcttacgaa
     4501 accacacctg ggcacttgtc acaaatttta tatgcagata tttcagttgc ctaatgaaca
     4561 aaccaagaca ctgatgtttt ttgaaggttg tccacagcac ctaggctgta caatcaagct
     4621 aagaggaggc tctgattatg agctggctcg agttaaggag atcctaatat ttatgatctg
     4681 tgttgcttat cattctcaac tagaaatatc ctttctcatg gatgaatttg ctatgcctcc
     4741 cacattaatg caaaaccctt cattccattc cctgattgag ggacgagggc atgagggggc
     4801 tgtccaagag cagtacggtg gaggttccat cccctgggat cctgacatcc ctcctgagtc
     4861 tctgccctgt gatgatagca gtttgctgga attgaggatt gtgtttgaga agggtgagca
     4921 ggaaaataaa aatcttccgc aggctgttgc ctctgtgaag catcaagaac atagcacaac
     4981 agcttgcccg gcgggtctcc cttgtgcttt ctttgcacct gtaccggaat cattgttgcc
     5041 actccctgtg gatgaccaac aagatgcttt aggcagcgag cagccagaga ctttgcagca
     5101 aacagttgtg ctgcaggatc ccaaaagcca gataagagcc tttagagacc ctctacagga
     5161 tgacactgga ttatatgtta ctgaggaagt cacctcctct gaagataaac gaaagactta
     5221 ttctttggcc tttaagcagg aattaaaaga tgtgatcctc tgtatctccc cagtaatcac
     5281 attccgagaa ccctttcttt taactgaaaa ggggatgaga tgctctaccc gagattattt
     5341 tgcagagcag gtttactggt ctcctctcct caataaagaa ttcaaagaaa tggagaacag
     5401 gaggaagaaa cagctgctca gggatctctc tggacttcag ggcatgaatg gaagtattca
     5461 ggccaagtct attcaagtct taccctcaca tgagctagtg agcactagaa ttgctgagca
     5521 tctgggcgat agccagagct tgggtagaat gctggccgat tatcgagcca gaggaggaag
     5581 aattcagccc aaaaattcag acccttttgc tcattcaaag gatgcatcaa gtacttcaag
     5641 tggccaatca ggaagcaaaa atgagggtga tgaagagaga gggcttattc tgagtgatgc
     5701 tgtgtggtca acaaaggtgg actgtctgaa tcccattaat caccagagac tttgtgtgct
     5761 cttcagcagc tcttctgccc agtccagcaa tgctcctagt gcctgtgtca gtccttggat
     5821 tgtaacaatg gaattttatg gaaagaatga tcttacatta ggaatatttt tagagagata
     5881 ctgtttcagg ccttcttatc agtgtccaag catgttctgt gataccccca tggtacatca
     5941 tattcggcgc tttgttcatg gccaaggctg tgtgcagata atcctgaagg agttggattc
     6001 tccagtacct ggatatcagc atacaattct tacatattcc tggtgtagaa tctgcaaaca
     6061 ggtaacacca gttgttgctc tttccaatga gtcctggtct atgtcatttg caaaatacct
     6121 tgaacttagg ttttatgggc accagtatac tcgcagagcc aacgctgagc cctgtggtca
     6181 ctccatccat catgattatc accagtattt ctcctataac cagatggtgg cgtctttcag
     6241 ttattctccc attcggcttc ttgaagtatg tgttccactc cccaaaatat tcattaagcg
     6301 tcaggcccca ttaaaagtgt cccttcttca ggatctgaag gacttctttc aaaaagtttc
     6361 acaggtatat gttgccattg atgaaagact tgcatctttg aaaactgata catttagtaa
     6421 aacaagagag gaaaaaatgg aagatatttt tgcacagaaa gagatggaag aaggtgagtt
     6481 caagaactgg attgagaaga tgcaagcaag gctcatgtct tcctctgtag atacccctca
     6541 gcaactgcag tcggtctttg agtcactcat tgccaagaaa caaagtctct gtgaagtgct
     6601 gcaagcttgg aataacaggt tgcaggacct tttccaacag gaaaagggta gaaagagacc
     6661 ttcagttcct ccaagtcctg gaagactgag acaaggggaa gaaagcaaga taagtgcgat
     6721 ggatgcatct ccacggaata tttctccagg acttcagaat ggagaaaaag aggatcgctt
     6781 cttaacaact ttgtccagcc agagctccac cagttctact catctccaat tgcctacgcc
     6841 acctgaagtc atgtctgaac agtcagtggg agggccccct gagctagata cagccagcag
     6901 ttccgaagat gtgtttgatg ggcatttgct gggatccaca gacagccaag tgaaggaaaa
     6961 gtcaaccatg aaagccatct ttgcaaattt gcttccagga aatagctata atcctattcc
     7021 atttcctttt gatccagata aacactactt aatgtatgaa catgaacgag tgcccattgc
     7081 agtctgcgag aaggaaccca gctccatcat tgcttttgct ctcagttgta aagaataccg
     7141 aaatgcctta gaggaattgt ctaaagcgac tcagtggaac agtgccgaag aagggcttcc
     7201 aacaaatagt acttcagata gcagaccaaa gagtagcagc cctatcagat tacctgaaat
     7261 gagtggagga cagacaaatc gtacaacaga aacagaacca caaccaacca aaaaggcttc
     7321 tggaatgttg tccttcttca gagggacagc agggaaaagc cccgatctct cttcccagaa
     7381 gagagagacc ttacgtggag cagatagtgc ttactaccag gttgggcaga cgggcaagga
     7441 ggggaccgag aatcaaggcg ttgagcctca agatgaagta gatggaggag atacacaaaa
     7501 gaagcaactc ataaatcctc atgtggaact tcaattttca gatgctaatg ccaagtttta
     7561 ctgtcggctc tactatgcgg gagagtttca taagatgcgt gaagtgattc tggacagcag
     7621 tgaagaagat ttcattcgtt ccctctccca ctcatcaccc tggcaggccc ggggaggcaa
     7681 atcaggagct gccttctatg caactgagga tgatagattt attttgaagc aaatgcctcg
     7741 tctggaagtc cagtccttcc tcgactttgc accacattac ttcaattata ttacaaatgc
     7801 tgttcaacaa aagaggccca cggcgttggc caaaattctt ggagtttaca gaattggtta
     7861 taagaactct cagaacaaca ctgagaagaa gttagatctc cttgtcatgg aaaatctttt
     7921 ctacgggaga aagatggcac aggtttttga tttgaagggc tctcttagga atcggaatgt
     7981 aaaaactgac actggaaaag agagttgtga tgtggtcctg ctagatgaaa atctcctaaa
     8041 gatggttcga gacaaccctc tatatattcg ttctcattcc aaagctgtgc tgagaacctc
     8101 gatccatagt gactcccatt tcctttctag ccacctcatt atagattatt ctttgctggt
     8161 tgggcgagat gatactagca atgagctagt agttggaatt atagattata ttcgaacatt
     8221 tacatgggac aaaaagcttg agatggttgt gaaatcaaca ggaattttag gtggacaagg
     8281 taaaatgcca acagtggtgt ctccggagtt gtacaggact aggttttgtg aggcaatgga
     8341 caagtatttc ctaatggtac cagaccactg gacaggcttg ggtctgaatt gctgaaatca
     8401 agctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8461 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8641 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8701 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8761 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8821 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8881 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8941 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     9001 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     9061 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     9121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     9241 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9421 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9541 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     9601 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     9661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     9721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     9781 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     9841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     9901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     9961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    10021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    10081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    10141 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    10201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    10261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    10321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    10381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    10441 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    10501 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10561 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
    10621 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    10681 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
    10741 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
    10801 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
    10861 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
