LOCUS       sequence-237779-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ256
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2582..2629
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2636..2656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2681..4117,4307..6970)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          4118..4306
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             6971..7018
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7235..7300
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7934..7954
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7961..8212
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8213..8266
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     terminator      8462..8714
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8759..8882
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8899..8918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8900..8918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8923..8939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8923..8939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggattacaag gatgatgatg ataaggatta caaggatgat gatgataaga tggctccaaa
     2641 gaagaagaga aaggttggaa tccacggagt tccagctgct gataagaagt actctatcgg
     2701 acttgccatc ggaaccaact ctgttggatg ggctgttatc accgatgagt acaaggttcc
     2761 atctaagaag ttcaaggttc ttggaaacac cgatagacac tctatcaaga agaaccttat
     2821 cggtgctctt cttttcgatt ctggagagac cgctgaggct accagattga agagaaccgc
     2881 tagaagaaga tacaccagaa gaaagaacag aatctgctac cttcaggaaa tcttctctaa
     2941 cgagatggct aaggttgatg attctttctt ccacagactt gaggagtctt tccttgttga
     3001 ggaggataag aagcacgaga gacacccaat cttcggaaac atcgttgatg aggttgctta
     3061 ccacgagaag tacccaacca tctaccacct tagaaagaag ttggttgatt ctaccgataa
     3121 ggctgatctt agacttatct accttgctct tgctcacatg atcaagttca gaggacactt
     3181 ccttatcgag ggagacctta acccagataa ctctgatgtt gataagttgt tcatccagct
     3241 tgttcagacc tacaaccagc ttttcgagga gaacccaatc aacgcttctg gagttgatgc
     3301 taaggctatc ctttctgcta gactttctaa gtctcgtaga cttgagaacc ttatcgctca
     3361 gcttccagga gagaagaaga acggactttt cggaaacctt atcgctcttt ctcttggact
     3421 taccccaaac ttcaagtcta acttcgatct tgctgaggat gctaagttgc agctttctaa
     3481 ggatacctac gatgatgatc ttgataacct tcttgctcag atcggagatc agtacgctga
     3541 tcttttcctt gctgctaaga acctttctga tgctatcctt ctttctgaca tccttagagt
     3601 taacaccgag atcaccaagg ctccactttc tgcttctatg atcaagagat acgatgagca
     3661 ccaccaggat cttacccttt tgaaggctct tgttagacag cagcttccag agaagtacaa
     3721 ggaaatcttc ttcgatcagt ctaagaacgg atacgctgga tacatcgatg gaggagcttc
     3781 tcaggaggag ttctacaagt tcatcaagcc aatccttgag aagatggatg gaaccgagga
     3841 gcttcttgtt aagttgaaca gagaggatct tcttagaaag cagagaacct tcgataacgg
     3901 atctatccca caccagatcc accttggaga gcttcacgct atccttcgta gacaggagga
     3961 tttctaccca ttcttgaagg ataacagaga gaagatcgag aagatcctta ccttcagaat
     4021 cccatactac gttggaccac ttgctagagg aaactctcgt ttcgcttgga tgaccagaaa
     4081 gtctgaggag accatcaccc cttggaactt cgaggaggta agtttctgct tctacctttg
     4141 atatatatat aataattatc attaattagt agtaatataa tatttcaaat atttttttca
     4201 aaataaaaga atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt
     4261 aatttataac ttttctaata tatgaccaaa atttgttgat gtgcaggttg ttgataaggg
     4321 agcttctgct cagtctttca tcgagagaat gaccaacttc gataagaacc ttccaaacga
     4381 gaaggttctt ccaaagcact ctcttcttta cgagtacttc accgtttaca acgagcttac
     4441 caaggttaag tacgttaccg agggaatgag aaagccagct ttcctttctg gagagcagaa
     4501 gaaggctatc gttgatcttc ttttcaagac caacagaaag gttaccgtta agcagttgaa
     4561 ggaggattac ttcaagaaga tcgagtgctt cgattctgtt gaaatctctg gagttgagga
     4621 tagattcaac gcttctcttg gaacctacca cgatcttttg aagatcatca aggataagga
     4681 tttccttgat aacgaggaga acgaggacat ccttgaggac atcgttctta cccttaccct
     4741 tttcgaggat agagagatga tcgaggagag actcaagacc tacgctcacc ttttcgatga
     4801 taaggttatg aagcagttga agagaagaag atacaccgga tggggtagac tttctcgtaa
     4861 gttgatcaac ggaatcagag ataagcagtc tggaaagacc atccttgatt tcttgaagtc
     4921 tgatggattc gctaacagaa acttcatgca gcttatccac gatgattctc ttaccttcaa
     4981 ggaggacatc cagaaggctc aggtttctgg acagggagat tctcttcacg agcacatcgc
     5041 taaccttgct ggatctccag ctatcaagaa gggaatcctt cagaccgtta aggttgttga
     5101 tgagcttgtt aaggttatgg gtagacacaa gccagagaac atcgttatcg agatggctag
     5161 agagaaccag accacccaga agggacagaa gaactctcgt gagagaatga agagaatcga
     5221 ggagggaatc aaggagcttg gatctcaaat cttgaaggag cacccagttg agaacaccca
     5281 gcttcagaac gagaagttgt acctttacta ccttcagaac ggaagagata tgtacgttga
     5341 tcaggagctt gacatcaaca gactttctga ttacgatgtt gatcacatcg ttccacagtc
     5401 tttcttgaag gatgattcta tcgataacaa ggttcttacc cgttctgata agaacagagg
     5461 aaagtctgat aacgttccat ctgaggaggt tgttaagaag atgaagaact actggagaca
     5521 gcttcttaac gctaagttga tcacccagag aaagttcgat aaccttacca aggctgagag
     5581 aggaggactt tctgagcttg ataaggctgg attcatcaag agacagcttg ttgagaccag
     5641 acagatcacc aagcacgttg ctcagatcct tgattctcgt atgaacacca agtacgatga
     5701 gaacgataag ttgatcagag aggttaaggt tatcaccttg aagtctaagt tggtttctga
     5761 tttcagaaag gatttccagt tctacaaggt tagagagatc aacaactacc accacgctca
     5821 cgatgcttac cttaacgctg ttgttggaac cgctcttatc aagaagtacc caaagttgga
     5881 gtctgagttc gtttacggag attacaaggt ttacgatgtt agaaagatga tcgctaagtc
     5941 tgagcaggag atcggaaagg ctaccgctaa gtacttcttc tactctaaca tcatgaactt
     6001 cttcaagacc gagatcaccc ttgctaacgg agagatcaga aagagaccac ttatcgagac
     6061 caacggagag accggagaga tcgtttggga taagggaaga gatttcgcta ccgttagaaa
     6121 ggttctttct atgccacagg ttaacatcgt taagaaaacc gaggttcaga ccggaggatt
     6181 ctctaaggag tctatccttc caaagagaaa ctctgataag ttgatcgcta gaaagaagga
     6241 ttgggaccca aagaagtacg gaggattcga ttctccaacc gttgcttact ctgttcttgt
     6301 tgttgctaag gttgagaagg gaaagtctaa gaagttgaag tctgttaagg agcttcttgg
     6361 aatcaccatc atggagcgtt cttctttcga gaagaaccca atcgatttcc ttgaggctaa
     6421 gggatacaag gaggttaaga aggatcttat catcaagttg ccaaagtact ctcttttcga
     6481 gcttgagaac ggaagaaaga gaatgcttgc ttctgctgga gagcttcaga agggaaacga
     6541 gcttgctctt ccatctaagt acgttaactt cctttacctt gcttctcact acgagaagtt
     6601 gaagggatct ccagaggata acgagcagaa gcagcttttc gttgagcagc acaagcacta
     6661 ccttgatgag atcatcgagc aaatctctga gttctctaag agagttatcc ttgctgatgc
     6721 taaccttgat aaggttcttt ctgcttacaa caagcacaga gataagccaa tcagagagca
     6781 ggctgagaac atcatccacc ttttcaccct taccaacctt ggtgctccag ctgctttcaa
     6841 gtacttcgat accaccatcg atagaaaaag atacacctct accaaggagg ttcttgatgc
     6901 tacccttatc caccagtcta tcaccggact ttacgagacc agaatcgatc tttctcagct
     6961 tggaggagat aagagaccag ctgctaccaa gaaggctgga caggctaaga agaagaaggg
     7021 agacgacgga ggtggcggta caggaggggg tgggtccgct gagtatgtca gggcgttgtt
     7081 cgacttcaat ggaaacgacg aggaagatct gccttttaaa aagggagata ttctcaggat
     7141 cagagataag ccggaagaac aatggtggaa cgctgaagac tctgaaggta agagaggtat
     7201 gattcttgtc ccctacgtcg agaagtattc gggtgactat aaagaccacg atggagatta
     7261 taaggaccac gatatagatt ataaggatga tgatgataag agcggaatga ccgatgcaga
     7321 gtacgtcagg attcatgaga aacttgacat ctacacgttt aagaaacagt ttttcaacaa
     7381 caaaaaatct gttagtcacc gctgttacgt gctgttcgaa ttgaaacgca gaggtgagag
     7441 gagagcctgc ttttggggct atgccgtcaa caagccgcaa agcggcacag aaaggggcat
     7501 tcacgcggag atatttagca ttagaaaggt cgaggaatac cttcgggata atcccgggca
     7561 attcactatc aattggtact cttcatggtc cccgtgtgca gattgcgctg aaaagatact
     7621 ggagtggtat aatcaagaac tcagaggaaa cggtcacacc ctcaagattt gggcttgcaa
     7681 gctttactac gagaaaaatg caaggaacca gatcggcctc tggaacttgc gcgacaacgg
     7741 cgtggggttg aatgtgatgg tctcggagca ttaccagtgc tgccggaaga tattcattca
     7801 gtcgtcacat aatcaattga acgagaatag gtggctcgaa aaaaccctga agcgggccga
     7861 gaagtggagg agtgaactct cgataatgat ccaggttaaa atactgcata ctaccaaatc
     7921 tccggcggtg ggaccgaaga agaagcgcaa ggtggggacc atgactaatc tctcagatat
     7981 aatcgagaag gaaacaggaa agcaactggt catccaagaa tcgattttga tgcttcccga
     8041 agaagtcgaa gaagttatag gaaataagcc cgagtctgac atactggttc acacagcgta
     8101 cgatgaaagt acggacgaga atgtcatgtt gctgacatcg gacgcacctg aatacaagcc
     8161 ttgggctctg gtcatacaag atagtaacgg agaaaataag attaaaatgc ttgaaggaag
     8221 gggctccctg ctgacatgtg gagatgtgga agagaatcca ggaccatcca ggatgataga
     8281 gcaagatggt tgtacccaag tgctgcggcc cctcggatgg cgggggtaca gcgccatgac
     8341 tggccataat agacagtccg ctgctctcat gcctccatgc agcggatgtc agaggagggg
     8401 agctaggttc ttcctgtcta gacctacctg ccccgtgccc tagtggcggc cgccgacgtc
     8461 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     8521 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     8581 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     8641 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     8701 tatgttacta gatcgggaat tgatcccccc tcgacagctt ccggaaaggg cgaattcgca
     8761 actttgtata caaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     8821 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     8881 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     8941 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     9001 tcatgcctcc tc
//
