LOCUS       sequence-237780-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ255-x3.7
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2576..3262
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             3317..3364
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             3371..3391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3416..7516
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7517..7564
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7586..7834
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7847..7867
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7889..8141
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8186..8309
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8326..8345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8327..8345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8350..8366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8350..8366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgag
     2581 ttctgaaaca ggtccagtgg ctgtcgatcc gactctgagg aggcgcatcg aaccacacga
     2641 atttgaggtt ttttttgatc caagagagct tcgcaaggaa acttgtctcc tgtatgagat
     2701 aaattgggga ggaagacata gtatctggag acacacaagc cagaacacca ataagcatgt
     2761 cgaagttaac ttcatcgaaa aatttacgac cgaacgctac ttttgtccta atacacgctg
     2821 cagtatcact tggttccttt cgtggtcacc ttgcggtgaa tgttccagag caattacgga
     2881 gtttctctcg agatatccac acgttacact tttcatttac atagctcggc tttatcatca
     2941 cgcagatccg agaaatcgcc aggggctgcg cgatctgatt tcgtccggtg tcaccattca
     3001 aattatgact gagcaggaat ctggatactg ctggcgcaac ttcgtgaact acagtccgtc
     3061 gaatgaagcg cactggccga gatatcccca tctctgggtg cgcttgtatg ttttggagct
     3121 ttattgtatc atcttgggat tgccaccttg tcttaatata ttgcgccgga aacaaccgca
     3181 gctcactttt ttcaccattg ctctgcagtc ttgtcactac caacgcctcc ccccgcacat
     3241 cctgtgggcc acaggcctta aatcaggttc tgagacccct ggcacaagtg agtcagcaac
     3301 acccgagtcc accatggatt acaaggatga tgatgataag gattacaagg atgatgatga
     3361 taagatggct ccaaagaaga agagaaaggt tggaatccac ggagttccag ctgctgataa
     3421 gaagtactct atcggacttg ccatcggaac caactctgtt ggatgggctg ttatcaccga
     3481 tgagtacaag gttccatcta agaagttcaa ggttcttgga aacaccgata gacactctat
     3541 caagaagaac cttatcggtg ctcttctttt cgattctgga gagaccgctg aggctaccag
     3601 attgaagaga accgctagaa gaagatacac cagaagaaag aacagaatct gctaccttca
     3661 ggaaatcttc tctaacgaga tggctaaggt tgatgattct ttcttccaca gacttgagga
     3721 gtctttcctt gttgaggagg ataagaagca cgagagacac ccaatcttcg gaaacatcgt
     3781 tgatgaggtt gcttaccacg agaagtaccc aaccatctac caccttagaa agaagttggt
     3841 tgattctacc gataaggctg atcttagact tatctacctt gctcttgctc acatgatcaa
     3901 gttcagagga cacttcctta tcgagggaga ccttaaccca gataactctg atgttgataa
     3961 gttgttcatc cagcttgttc agacctacaa ccagcttttc gaggagaacc caatcaacgc
     4021 ttctggagtt gatgctaagg ctatcctttc tgctagactt tctaagtctc gtagacttga
     4081 gaaccttatc gctcagcttc caggagagaa gaagaacgga cttttcggaa accttatcgc
     4141 tctttctctt ggacttaccc caaacttcaa gtctaacttc gatcttgctg aggataccaa
     4201 gttgcagctt tctaaggata cctacgatga tgatcttgat aaccttcttg ctcagatcgg
     4261 agatcagtac gctgatcttt tccttgctgc taagaacctt tctgatgcta tccttctttc
     4321 tgacatcctt agagttaaca ccgagatcac caaggctcca ctttctgctt ctatgatcaa
     4381 gctctacgat gagcaccacc aggatcttac ccttttgaag gctcttgtta gacagcagct
     4441 tccagagaag tacaaggaaa tcttcttcga tcagtctaag aacggatacg ctggatacat
     4501 cgatggagga gcttctcagg aggagttcta caagttcatc aagccaatcc ttgagaagat
     4561 ggatggaacc gaggagcttc ttgttaagtt gaacagagag gatcttctta gaaagcagag
     4621 aaccttcgat aacggaatca tcccacacca gatccacctt ggagagcttc acgctatcct
     4681 tcgtagacag gaggatttct acccattctt gaaggataac agagagaaga tcgagaagat
     4741 ccttaccttc agaatcccat actacgttgg accacttgct agaggaaact ctcgtttcgc
     4801 ttggatgacc agaaagtctg aggagaccat caccccttgg aacttcgaga aggttgttga
     4861 taagggagct tctgctcagt ctttcatcga gagaatgacc aacttcgata agaaccttcc
     4921 aaacgagaag gttcttccaa agcactctct tctttacgag tacttcaccg tttacaacga
     4981 gcttaccaag gttaagtacg ttaccgaggg aatgagaaag ccagctttcc tttctggaga
     5041 ccagaagaag gctatcgttg atcttctttt caagaccaac agaaaggtta ccgttaagca
     5101 gttgaaggag gattacttca agaagatcga gtgcttcgat tctgttgaaa tctctggagt
     5161 tgaggataga ttcaacgctt ctcttggaac ctaccacgat cttttgaaga tcatcaaaga
     5221 taaggacttc cttgataacg aagagaacga ggatatcctc gaggacatcg ttcttacctt
     5281 gacccttttc gaggatagag agatgatcga ggagagactc aagacctacg ctcacctttt
     5341 cgatgataag gttatgaagc agttgaagag aagaagatac accggatggg gtagactttc
     5401 tcgtaagttg atcaacggaa tcagagataa gcagtctgga aagaccatcc ttgatttctt
     5461 gaagtctgat ggattcgcta acagaaactt catccagctt atccacgatg attctcttac
     5521 cttcaaggag gacatccaga aggctcaggt ttctggacag ggagattctc ttcacgagca
     5581 catcgctaac cttgctggat ctccagctat caagaaggga atccttcaga ccgttaaggt
     5641 tgttgatgag cttgttaagg ttatgggtag acacaagcca gagaacatcg ttatcgagat
     5701 ggctagagag aaccagacca cccagaaggg acagaagaac tctcgtgaga gaatgaagag
     5761 aatcgaggag ggaatcaagg agcttggatc tcaaatcttg aaggagcacc cagttgagaa
     5821 cacccagctt cagaacgaga agttgtacct ttactacctt cagaacggaa gagatatgta
     5881 cgttgatcag gagcttgaca tcaacagact ttctgattac gatgttgatc acatcgttcc
     5941 acagtctttc ttgaaggatg attctatcga taacaaggtt cttacccgtt ctgataagaa
     6001 cagaggaaag tctgataacg ttccatctga ggaggttgtt aagaagatga agaactactg
     6061 gagacagctt cttaacgcta agttgatcac ccagagaaag ttcgataacc ttaccaaggc
     6121 tgagagagga ggactttctg agcttgataa ggctggattc atcaagagac agcttgttga
     6181 gaccagacag atcaccaagc acgttgctca gatccttgat tctcgtatga acaccaagta
     6241 cgatgagaac gataagttga tcagagaggt taaggttatc accttgaagt ctaagttggt
     6301 ttctgatttc agaaaggatt tccagttcta caaggttaga gagatcaaca actaccacca
     6361 cgctcacgat gcttacctta acgctgttgt tggaaccgct cttatcaaga agtacccaaa
     6421 gttggagtct gagttcgttt acggagatta caaggtttac gatgttagaa agatgatcgc
     6481 taagtctgag caggagatcg gaaaggctac cgctaagtac ttcttctact ctaacatcat
     6541 gaacttcttc aagaccgaga tcacccttgc taacggagag atcagaaaga gaccacttat
     6601 cgagaccaac ggagagaccg gagagatcgt ttgggataag ggaagagatt tcgctaccgt
     6661 tagaaaggtt ctttctatgc cacaggttaa catcgttaag aaaaccgagg ttcagaccgg
     6721 aggattctct aaggagtcta tccttccaaa gagaaactct gataagttga tcgctagaaa
     6781 gaaggattgg gacccaaaga agtacggagg attcgattct ccaaccgttg cttactctgt
     6841 tcttgttgtt gctaaggttg agaagggaaa gtctaagaag ttgaagtctg ttaaggagct
     6901 tcttggaatc accatcatgg agcgttcttc tttcgagaag aacccaatcg atttccttga
     6961 ggctaaggga tacaaggagg ttaagaagga tcttatcatc aagttgccaa agtactctct
     7021 tttcgagctt gagaacggaa gaaagagaat gcttgcttct gctggagtgc ttcagaaggg
     7081 aaacgagctt gctcttccat ctaagtacgt taacttcctt taccttgctt ctcactacga
     7141 gaagttgaag ggatctccag aggataacga gcagaagcag cttttcgttg agcagcacaa
     7201 gcactacctt gatgagatca tcgagcaaat ctctgagttc tctaagagag ttatccttgc
     7261 tgatgctaac cttgataagg ttctttctgc ttacaacaag cacagagata agccaatcag
     7321 agagcaggct gagaacatca tccacctttt cacccttacc aaccttggtg ctccagctgc
     7381 tttcaagtac ttcgatacca ccatcgatag aaaaagatac acctctacca aggaggttct
     7441 tgatgctacc cttatccacc agtctatcac cggactttac gagaccagaa tcgatctttc
     7501 tcagcttgga ggagataaga gaccagctgc taccaagaag gctggacagg ctaagaagaa
     7561 gaagggagac ggatcaggcg ggtcaacaaa cctcagtgac atcatagaga aggaaactgg
     7621 taagcaactg gttattcaag agagcattct gatgctccct gaggaagtcg aggaagttat
     7681 aggaaacaag cctgagagcg atatactcgt gcatacggcg tatgacgaga gcacggatga
     7741 aaatgtgatg ctcttgacca gcgatgcgcc agaatataaa ccctgggcac tggttattca
     7801 ggactctaac ggggaaaata aaatcaagat gctctcaggt ggctccccaa agaagaaacg
     7861 caaggtttag tggcggccgc cgacgtccga tcgttcaaac atttggcaat aaagtttctt
     7921 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     7981 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     8041 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     8101 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattga tcccccctcg
     8161 acagcttccg gaaagggcga attcgcaact ttgtatacaa aagttgaacg agaaacgtaa
     8221 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     8281 tgtaaaacac aacatatcca gtcactatgc catccagctg atatccccta tagtgagtcg
     8341 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     8401 tacattgcac aagataaaaa tatatcatca tgcctcctc
//
