LOCUS       sequence-237791-       10553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C terminal domain of hnRNPA1 (186-320) tagged with SspB and 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher 
            JE, Brangwynne CP
  TITLE     Mapping Local and Global Liquid Phase Behavior in Living Cells Using
            Photo-Oligomerizable Seeds.
  JOURNAL   Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 
            10.1016/j.cell.2018.10.048.
  PUBMED    30500534
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1467-1480.e13"
COMMENT     Sequence Label: M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
FEATURES             Location/Qualifiers
     source          1..10553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    748..865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1018..1425
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1739..1750
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2679..3008
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    3115..3703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3168..3188)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3586..3597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3706..3722)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3707..3723)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3813..4046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4071..4089)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4072..4089)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4379..4398)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4612..4631)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4731..4753
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4791..4809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4877..4981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4982..5050,5051..5842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5200..5219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6013..6601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6502..6521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6755..6772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6842..6862)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6847..7176
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7027..7162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8310..8375
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8505..8525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8950..9084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8987..9006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9041..9060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9252..9885
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9932..10057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      781 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      841 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
      901 gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat tttatttagt
      961 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     1021 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     1081 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     1141 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     1201 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     1261 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     1321 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     1381 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga tctggagctc
     1441 tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatca ctagtcggag
     1501 ctaccatggc tagtgcttca tccagccaaa gaggtcgaag tggttctgga aactttggtg
     1561 gtggtcgtgg aggtggtttc ggtgggaatg acaacttcgg tcgtggagga aacttcagtg
     1621 gtcgtggtgg ctttggtggc agccgtggtg gtggtggata tggtggcagt ggggatggct
     1681 ataatggatt tggtaatgat ggaagcaatt ttggaggtgg tggaagctac aatgattttg
     1741 ggaattacaa caatcagtct tcaaattttg gacccatgaa gggaggaaat tttggaggca
     1801 gaagctctgg cccctatggc ggtggaggcc aatactttgc aaaaccacga aaccaaggtg
     1861 gctatggcgg ttccagcagc agcagtagct atggcagtgg cagaagattt ggtggatccg
     1921 gaggcggcgg atccggcgga atggtgtcta aaggcgagga ggataacatg gctataatca
     1981 aggaatttat gaggttcaag gtgcatatgg agggatctgt gaacggtcac gagttcgaaa
     2041 tcgagggcga aggcgagggg cgaccctatg aagggacaca gacggccaaa ctgaaggtga
     2101 ccaagggtgg ccccctgcct ttcgcctggg acatcctgtc accccagttt atgtacggct
     2161 ctaaggctta cgttaagcac cctgccgata ttccggacta cttgaaactg agcttccctg
     2221 aagggttcaa gtgggaacgg gtaatgaatt ttgaggatgg cggtgtagtc acagttaccc
     2281 aggacagctc tcttcaggac ggagaattta tctataaggt taaacttcgg ggcactaact
     2341 tcccatcaga cggccccgtg atgcagaaga aaactatggg ctgggaagcc agctcagaac
     2401 gcatgtatcc cgaggacgga gcactgaagg gagaaatcaa gcagcgactc aagctgaagg
     2461 atgggggaca ttatgatgct gaggtcaaaa ccacctataa ggccaagaaa cccgttcagc
     2521 ttcctggggc atacaacgtg aatatcaaac tggatattac atcccataac gaagactata
     2581 ccatcgtgga acagtacgag cgggccgagg gcagacatag cacagggggc atggatgaat
     2641 tgtacaaggc ggccggatcc ggtggtacct ctggaggtag ctccccgaaa cgccctaagc
     2701 tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat ctggtggtgg
     2761 atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt cagatcgtgc
     2821 tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt atccagttca
     2881 acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc gctctggcca
     2941 tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc tatgacgagc
     3001 tgaatattgg ttaaggatcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc
     3061 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     3121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3481 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     3721 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     3781 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3841 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3901 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3961 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4021 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     4081 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     4141 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     4201 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     4261 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     4321 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     4381 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     4441 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     4501 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     4561 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     4621 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4681 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4741 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4801 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4861 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4921 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4981 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5041 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5101 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5161 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5221 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5281 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5341 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5401 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5461 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5521 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5581 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggaa cacttctgcg
     5641 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5701 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5761 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5821 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5881 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5941 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6781 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6841 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6901 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7021 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7081 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7141 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     7201 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     7261 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     7321 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     7381 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     7441 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     7501 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     7561 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     7621 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     7681 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     7741 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     7801 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     7861 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     7921 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     7981 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     8041 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     8101 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     8161 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     8221 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     8281 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     8341 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     8401 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     8461 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     8521 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     8581 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     8641 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     8701 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     8761 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     8821 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     8881 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8941 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9061 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     9121 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     9181 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     9241 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     9301 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     9361 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     9421 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     9481 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     9541 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     9601 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     9661 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     9721 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9781 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9841 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9901 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9961 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10021 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10081 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10141 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10201 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10261 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10321 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10381 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10441 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10501 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aat
//
