LOCUS       sequence-237792-       10733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C terminal domain of TDP43 (218-414) tagged with SspB and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher 
            JE, Brangwynne CP
  TITLE     Mapping Local and Global Liquid Phase Behavior in Living Cells Using
            Photo-Oligomerizable Seeds.
  JOURNAL   Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 
            10.1016/j.cell.2018.10.048.
  PUBMED    30500534
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1467-1480.e13"
COMMENT     Sequence Label: M31: pHR-SFFVp- TDP-43C::mCherry::SspB
FEATURES             Location/Qualifiers
     source          1..10733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        153..560
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1994..2323
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    2430..3018
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2483..2503)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2901..2912)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3021..3037)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3022..3038)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3128..3361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3386..3404)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3387..3404)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3694..3713)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3927..3946)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4046..4068
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4106..4124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4192..4296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4297..4365,4366..5157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4515..4534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5328..5916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5817..5836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6070..6087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6157..6177)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6162..6491
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6342..6477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7625..7690
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7820..7840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8265..8399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8302..8321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8356..8375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8567..9200
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9247..9372
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9869..10102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10287..10331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10616..10733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc
       61 ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
      121 accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag gcatggaaaa
      181 ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa tagctaacgt
      241 tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc caagaacaga
      301 tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc agatatggcc
      361 caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca aggacctgaa
      421 atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc
      481 ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg ccagtcctcc
      541 gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg cgtcaagtgg
      601 agcaaggcag gtggacagtg gatcactagt cggagctacc atggatgtct tcatccccaa
      661 gccattcagg gcctttgcct ttgttacatt tgcagatgat cagattgcgc agtctctttg
      721 tggagaggac ttgatcatta aaggaatcag cgttcatata tccaatgccg aacctaagca
      781 caatagcaat agacagttag aaagaagtgg aagatttggt ggtaatccag gtggctttgg
      841 gaatcagggt ggatttggta atagcagagg gggtggagct ggtttgggaa acaatcaagg
      901 tagtaatatg ggtggtggga tgaactttgg tgcgttcagc attaatccag ccatgatggc
      961 tgccgcccag gcagcactac agagcagttg gggtatgatg ggcatgttag ccagccagca
     1021 gaaccagtca ggcccatcgg gtaataacca aaaccaaggc aacatgcaga gggagccaaa
     1081 ccaggccttc ggttctggaa ataactctta tagtggctct aattctggtg cagcaattgg
     1141 ttggggatca gcatccaatg cagggtcggg cagtggtttt aatggaggct ttggctcaag
     1201 catggattct aagtcttctg gctggggaat gtccggaggc ggcggatccg gcggaatggt
     1261 gtctaaaggc gaggaggata acatggctat aatcaaggaa tttatgaggt tcaaggtgca
     1321 tatggaggga tctgtgaacg gtcacgagtt cgaaatcgag ggcgaaggcg aggggcgacc
     1381 ctatgaaggg acacagacgg ccaaactgaa ggtgaccaag ggtggccccc tgcctttcgc
     1441 ctgggacatc ctgtcacccc agtttatgta cggctctaag gcttacgtta agcaccctgc
     1501 cgatattccg gactacttga aactgagctt ccctgaaggg ttcaagtggg aacgggtaat
     1561 gaattttgag gatggcggtg tagtcacagt tacccaggac agctctcttc aggacggaga
     1621 atttatctat aaggttaaac ttcggggcac taacttccca tcagacggcc ccgtgatgca
     1681 gaagaaaact atgggctggg aagccagctc agaacgcatg tatcccgagg acggagcact
     1741 gaagggagaa atcaagcagc gactcaagct gaaggatggg ggacattatg atgctgaggt
     1801 caaaaccacc tataaggcca agaaacccgt tcagcttcct ggggcataca acgtgaatat
     1861 caaactggat attacatccc ataacgaaga ctataccatc gtggaacagt acgagcgggc
     1921 cgagggcaga catagcacag ggggcatgga tgaattgtac aaggcggccg gatccggtgg
     1981 tacctctgga ggtagctccc cgaaacgccc taagctgctg cgtgaatatt acgattggct
     2041 ggttgataac agctttaccc catatctggt ggtggatgcc acatacctgg gcgtgaacgt
     2101 gcccgtggag tatgtgaaag acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg
     2161 caacctgcaa ctgacaaatg attttatcca gttcaacgcc cgctttaagg gcgtgtctcg
     2221 tgaactgtat atcccgatgg gtgccgctct ggccatttac gctcgcgaga acggcgatgg
     2281 tgtgatgttc gaaccagaag aaatctatga cgagctgaat attggttaag gatcggccgc
     2341 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg
     2401 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     2461 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2521 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2581 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2641 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2701 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2761 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2821 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2881 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2941 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3001 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     3061 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3121 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     3421 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     3481 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     3541 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     3601 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     3661 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     3721 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     3781 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     3841 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     3901 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3961 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4021 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4081 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4141 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4201 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4261 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4321 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4381 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4441 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4501 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4561 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4621 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4681 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4741 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4801 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4861 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4921 atggaggcgg ataaagttgc aggaacactt ctgcgctcgg cccttccggc tggctggttt
     4981 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5041 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5101 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5161 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5221 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5281 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5341 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5401 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5461 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5521 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5581 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5641 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5701 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5761 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5821 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5881 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5941 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6001 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6061 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6121 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     6181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6241 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6301 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6361 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6421 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6481 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     6541 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     6601 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     6661 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     6721 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     6781 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     6841 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     6901 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     6961 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     7021 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     7081 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     7141 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     7201 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     7261 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     7321 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     7381 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     7441 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     7501 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     7561 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     7621 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     7681 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     7741 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     7801 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     7861 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     7921 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     7981 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     8041 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     8101 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     8161 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     8221 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     8281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     8401 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     8461 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     8521 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     8581 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8641 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8701 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8761 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8821 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8881 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8941 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9001 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9181 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9241 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9301 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9361 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9421 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9481 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9541 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9601 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9661 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9721 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9781 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9841 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9901 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9961 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10021 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10081 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10141 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10201 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10261 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10321 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10381 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10441 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10501 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10561 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
    10621 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10681 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat ttt
//
