LOCUS       sequence-237793-       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses disordered protein TAF15(1-208) fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-TAF15N-miRFP670-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             115..1059
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             1072..2565
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    2717..3305
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2770..2790)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3188..3199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3308..3324)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3309..3325)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3415..3648
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3673..3691)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3674..3691)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3981..4000)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4214..4233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4333..4355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4393..4411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4479..4583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4584..4652,4653..5444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4802..4821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5615..6203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6104..6123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6357..6374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6444..6464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6449..6778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6629..6764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7912..7977
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8107..8127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8552..8686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8589..8608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8643..8662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8854..9487
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9534..9659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10156..10389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10574..10618
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10903..11020
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11173..11580
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gggtcacagg gaggaggtag agggcgtggg ggatatgaca aggatggaag aggtcctatg
       61 acaggatcaa gtggtggtga ccgcggtggc ggaggcggct ccggaggcgg aagcatggta
      121 gcaggtcatg cctctggcag ccccgcattc gggaccgcct ctcattcgaa ttgcgaacat
      181 gaagagatcc acctcgccgg ctcgatccag ccgcatggcg cgcttctggt cgtcagcgaa
      241 catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg aatttctgaa tctcggaagc
      301 gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt tgatcaagat cctgccgcat
      361 ctcgatccca ccgccgaagg catgccggtc gcggtgcgct gccggatcgg caatccctct
      421 acggagtact gcggtctgat gcatcggcct ccggaaggcg ggctgatcat cgaactcgaa
      481 cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc cggcgctgga gcggatccgc
      541 acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc tgctgtttca gcagtgcacc
      601 ggctacgacc gggtgatggt gtatcgtttc gatgagcaag gccacggcct ggtattctcc
      661 gagtgccatg tgcctgggct cgaatcctat ttcggcaacc gctatccgtc gtcgactgtc
      721 ccgcagatgg cgcggcagct gtacgtgcgg cagcgcgtcc gcgtgctggt cgacgtcacc
      781 tatcagccgg tgccgctgga gccgcggctg tcgccgctga ccgggcgcga tctcgacatg
      841 tcgggctgct tcctgcgctc gatgtcgccg tgccatctgc agttcctgaa ggacatgggc
      901 gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca agctgtgggg cctggttgtc
      961 tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc gggcgatctg caaacggctc
     1021 gccgaaagga tcgcgacgcg gatcaccgcg cttgagagcg cggccacgcg tatgaagatg
     1081 gacaaaaaga ctatagtttg gtttagaaga gacctaagga ttgaggataa tcctgcatta
     1141 gcagcagctg ctcacgaagg atctgttttt cctgtcttca tttggtgtcc tgaagaagaa
     1201 ggacagtttt atcctggaag agcttcaaga tggtggatga aacaatcact tgctcactta
     1261 tctcaatcct tgaaggctct tggatctgac ctcactttaa tcaaaaccca caacacgatt
     1321 tcagcgatct tggattgtat ccgcgttacc ggtgctacaa aagtcgtctt taaccacctc
     1381 tatgatcctg tttcgttagt tcgggaccat accgtaaagg agaagctggt ggaacgtggg
     1441 atctctgtgc aaagctacaa tggagatcta ttgtatgaac cgtgggagat atactgcgaa
     1501 aagggcaaac cttttacgag tttcaattct tactggaaga aatgcttaga tatgtcgatt
     1561 gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa taactgcagc ggctgaagcg
     1621 atttgggcgt gttcgattga agaactaggg ctggagaatg aggccgagaa accgagcaat
     1681 gcgttgttaa ctagagcttg gtctccagga tggagcaatg ctgataagtt actaaatgag
     1741 ttcatcgaga agcagttgat agattatgca aagaacagca agaaagttgt tgggaattct
     1801 acttcactac tttctccgta tctccatttc ggggaaataa gcgtcagaca cgttttccag
     1861 tgtgcccgga tgaaacaaat tatatgggca agagataaga acagtgaagg agaagaaagt
     1921 gcagatcttt ttcttagggg aatcggttta agagagtatt ctcggtatat atgtttcaac
     1981 ttcccgttta ctcacgagca atcgttgttg agtcatcttc ggtttttccc ttgggatgct
     2041 gatgttgata agttcaaggc ctggagacaa ggcaggaccg gttatccgtt ggtggatgcc
     2101 ggaatgagag agctttgggc taccggatgg atgcataaca gaataagagt gattgtttca
     2161 agctttgctg tgaagtttct tctccttcca tggaaatggg gaatgaagta tttctgggat
     2221 acacttttgg atgctgattt ggaatgtgac atccttggct ggcagtatat ctctgggagt
     2281 atccccgatg gccacgagct tgatcgcttg gacaatcccg cgttacaagg cgccaaatat
     2341 gacccagaag gtgagtacat aaggcaatgg cttcccgagc ttgcgagatt gccaactgaa
     2401 tggatccatc atccatggga cgctccttta accgtactca aagcttctgg tgtggaactc
     2461 ggaacaaact atgcgaaacc cattgtagac atcgacacag ctcgtgagct actagctaaa
     2521 gctatttcaa gaacccgtga agcacagatc atgatcggag cagcagcccg ggatccaccg
     2581 gtcgccaccg gttcagggag cggatccggc tcagggagtg cggccgcaac tcccacctgc
     2641 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     2701 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2761 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2821 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2881 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2941 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3001 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3061 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3121 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3181 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3241 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3301 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     3361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3421 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     3661 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     3721 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     3781 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     3841 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     3901 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     3961 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     4021 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     4081 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     4141 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     4201 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4261 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4321 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4381 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4441 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4561 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4621 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4681 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4741 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4801 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4861 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4921 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4981 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5041 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5101 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5161 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5221 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5281 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5341 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5401 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5461 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5521 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5761 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6001 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6421 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6601 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6661 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6721 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6781 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     6841 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     6901 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     6961 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     7021 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     7081 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     7141 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     7201 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     7261 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     7321 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     7381 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     7441 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     7501 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     7561 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     7621 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     7681 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     7741 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     7801 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     7861 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     7921 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     7981 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     8041 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     8101 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     8161 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     8221 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     8281 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     8341 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     8401 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     8461 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     8521 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     8581 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8641 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8701 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     8761 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     8821 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     8881 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     8941 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9001 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9061 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9121 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9181 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     9241 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     9301 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9361 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9421 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9481 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9541 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9601 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9661 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9721 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9781 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9841 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9901 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9961 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
    10021 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    10081 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    10141 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    10201 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    10261 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    10321 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10381 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10441 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10501 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10561 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10621 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10681 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10741 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10801 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10861 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
    10921 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    10981 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    11041 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
    11101 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
    11161 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
    11221 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
    11281 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
    11341 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
    11401 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
    11461 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
    11521 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
    11581 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatgtcgga
    11641 ttctggaagt tacggtcagt ctgggggtga gcagcaaagt tattctacct atggaaatcc
    11701 aggcagccaa ggctatggac aagcatcaca aagctattct ggctatgggc aaacgactga
    11761 ttcctcttat ggacagaact acagcggtta ctccagttat ggacaaagtc agtcaggtta
    11821 ttcacagtcc tatggtggtt atgagaatca aaagcagagc tcatatagcc agcaaccata
    11881 taataaccag ggacagcagc aaaacatgga atcatcagga agccaaggtg gaagagcacc
    11941 ttcctatgac cagccagact atggtcaaca agattcatat gaccagcagt caggctatga
    12001 tcaacatcaa ggctcatatg atgagcagtc aaattatgat cagcagcatg attcctatag
    12061 tcaaaaccag cagtcctatc attcacaaag ggaaaactac agccaccaca cacaagatga
    12121 ccgtcgtgat gtgagtaggt atggagaaga taatagagga tatggc
//
