LOCUS       sequence-237797-        6516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6516)
  TITLE     AAV expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-Syn-iCre
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      1383..1392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1395..1415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2451..2477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2503..3091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2556..2576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2974..2985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3116..3237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3127..3146)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3181..3200
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3337..3466
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(3528..4116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3608..3627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4287..5078,5079..5147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4910..4929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5148..5252)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5320..5338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5376..5398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5498..5517
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5534..5989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5671..5692)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5883..5902
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   6064..6204
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6075..6204
                     /label=AAV2 ITR
ORIGIN
        1 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
       61 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      121 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      181 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      241 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      301 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat tcagaaccac
      361 ggacagcacc gaattcaatg tgtaatgtgt gctcaatatg ctcatgtgtg ttaccctgag
      421 cttctgtgtc tacatatata cctggatgcc tgtgtgttct gtgatgtaca tatatattct
      481 gtctttcctt ccttttctat ttgtgttatt ccatgtgttc tttcagattc tcaccaccaa
      541 gggcaaggat atgttaacta cccaagtgtc cacctccgcc tgtctggtga tgtttacgcc
      601 acccccgtgc tcttttcttt gcccgacaga gttgttatag gagatgtctc cccgggaaca
      661 ctgcaggaag gagaatttct acatttatgt tcccctctga gtgtgcttct atccccaaaa
      721 tgccttcaaa ggtgaaaatc aacactggaa acccaagtat ctgggaaggg caagagtgtg
      781 taagtgcaag ttagcctaag gaataggaag aggttggtaa acagggtagg atcgtgggag
      841 ggagtttcgt tactacaggt ccggaccctc aggacaagaa ccccaccccc actccccaaa
      901 ttgcgcatcc cccgccccca tcagaggggg aggggaagag gttgcggcgc ggcgcatgcg
      961 cactgtcgga ttcagcaccg cggtcagagc cttcgcctcc gctgccggcg cgcaccacca
     1021 cctccccagc accaaaggct gactgacgtc actcactagc cctccccaaa ctccccttcc
     1081 tcgccgcctt ggtcgcgtcc atgctgccgt gagtccagtc ggaccgcacc acgagaggtg
     1141 caagataggg aggtgcgggc gcgaccatac gctctgcggc ggcagagcct cggcgctgcc
     1201 tcagtctgca gcgggcagca gaggagtcgc gtcgtgccag agagcaccgc cgtgctcctg
     1261 agcccctcgc gctccgcccc cgcggcccac agacccactg ccccttggat cccctgcccc
     1321 acgccagagc ctctgctaac catgttcatg ccttcttctt tttcctacag gagctcgcca
     1381 ccgccaccat ggtgcccaag aagaagagga aagtctccaa cctgctgact gtgcaccaaa
     1441 acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca
     1501 tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca
     1561 gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct gaacctgagg
     1621 atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag accatccaac
     1681 agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc ccttctgact
     1741 ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat gctggggaga
     1801 gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga tccctgatgg
     1861 agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt gcctacaaca
     1921 ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc cgcaccgatg
     1981 gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca gctggtgtgg
     2041 agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct gtgtctggtg
     2101 tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt gtggctgccc
     2161 cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag gccacccacc
     2221 gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg tctggccact
     2281 ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc cctgaaatca
     2341 tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga aacctggact
     2401 ctgagactgg ggccatggtg aggctgctcg aggatgggga cagcggcgga tacccttacg
     2461 acgtgcctga ctacgcctga ctgcagaacg gccgttatcg ataatcaacc tctggattac
     2521 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2581 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2641 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2701 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2761 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2821 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2881 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2941 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3001 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3061 agtcggatct ccctttgggc cgcctccccg catcgattgc ggccgtcaga catgataaga
     3121 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3181 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3241 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
     3301 agcaagtaaa acctctacaa atgtggtagc ggccgcagga acccctagtg atggagttgg
     3361 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3421 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa
     3481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5281 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5341 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     5401 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     5461 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     5521 agagtgcacc ataaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     5581 taaatcagct cattttttaa ccaatagacc gaaatcggca aaatccctta taaatcaaaa
     5641 gaatagcccg agatagagtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5701 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5761 gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5821 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5881 gaagggaaga aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg
     5941 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta ctatggttgc
     6001 tttgacgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6061 gcccctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
     6121 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
     6181 ccaactccat cactaggggt tcctgcggcc gcggtacctt aattaaggga tgtaattacg
     6241 tccctaaccc gctagggggc agcagcgagc cgaacggggc tccgctccgg tccggcgcta
     6301 accccgcatc cccgaggggg gatgtaatta cgtccctaac ccgctagggg gcagcagcga
     6361 gccgaacggg gctccgctcc ggtccggcgc taaccccgca tccccgaggg gggatgtaat
     6421 tacgtcccta acccgctagg gggcagcagc gagccgaacg gggctccgct ccggtccggc
     6481 gctaaccccg catccccgag ggtcgactat taatag
//
