LOCUS       sequence-23780-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Lee K, Deng X, Friedman E
  TITLE     Mirk protein kinase is a mitogen-activated protein kinase substrate 
            that mediates survival of colon cancer cells.
  JOURNAL   Cancer Res. 2000 Jul 1. 60(13):3631-7.
  PUBMED    10910078
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2000 Jul 1. 60(13):3631-7."
COMMENT     Sequence Label: pcDNA-Mirk (YF)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             124..141
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..177
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(181..189,190..204)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttac catggggggt
      121 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg
      181 gatctgtacg acgatgacga taaggtacct aggatccagt gtggtggaat tctgcagatc
      241 ctcgctcccc tgcggccctc tgagcccacc atggccgtcc caccgggcca tggtcccttc
      301 tctggcttcc cagggcccca ggagcacacg caggtattgc ctgatgtgcg gctactgcct
      361 cggaggctgc ccctggcctt ccgggatgca acctcagccc cgctgcgtaa gctctctgtg
      421 gacctcatca agacctacaa gcacatcaat gaggtatact atgcgaagaa gaagcggcgg
      481 gcccagcagg cgccacccca ggattcgagc aacaagaagg agaagaaggt cctgaaccat
      541 ggttatgatg acgacaacca tgactacatc gtgcgcagtg gcgagcgctg gctggagcgc
      601 tacgaaattg actcgctcat tggcaaaggc tcctttggcc aggtggtgaa agcctatgat
      661 catcagaccc aggagcttgt ggccatcaag atcatcaaga acaaaaaggc tttcctgaac
      721 caggcccaga ttgagctgcg gctgctggag ctgatgaac
//
