LOCUS       sequence-237807-       14581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14581)
  AUTHORS   Sha F, Abdelfattah AS, Patel R, Schreiter ER
  TITLE     Erasable labeling of neuronal activity using a reversible calcium 
            marker.
  JOURNAL   Elife. 2020 Sep 15;9. pii: 57249. doi: 10.7554/eLife.57249.
  PUBMED    32931424
REFERENCE   2  (bases 1 to 14581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-09-15"; volume: "9. pii: 57249. doi"; pages: " 
            10.7554/eLife.57249"
COMMENT     Sequence Label: pTol2-elavl3_NES-rsCaMPARI-mRuby3
FEATURES             Location/Qualifiers
     source          1..14581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6561..6578
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7821..8528
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     polyA_signal    8573..8707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8597..8616)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8651..8670
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9146..9333,9334..9345)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9379..9395)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9379..9395)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9432..9452)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9432..9450)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9471..9487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9471..9487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9484..9506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9495..9511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9519..9525,9526..9543,9544..9549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9564..9585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9702..9719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9873..10461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9953..9972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10632..11423,11424..11492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11255..11274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11493..11597)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11760..11781)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(12130..12141,12142..12304)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 cattcaacac atggtaatat agaaacggtt atgtcgaggt tgttttgtcc agagatgacc
       61 ttcacacagt tacagccgct ctgcatccac acaaatggag gacttaatcg tggactgcat
      121 tcttagaaat gatctacaaa gacaaataat gtgaaatcaa gaaaggacaa aatttaagta
      181 aggggatgag ggagagagag aacgaggggc aaggagaaag catggctcct gtctttttct
      241 gcacccatct gttcggagtg caggtggagc tctattcact cagctctgca tgtgtgtttg
      301 gggggggcag gaagaaaggg agggcaaaag gaagagtgga gagatggtgg gggctggagg
      361 gatggggggt tctcggtgat ctctcctgaa ggggataatg ggagagcagc gctttgcaat
      421 ggctgccatg tagtaccctc cctgcacaat tagccaatca gcagcaagct ctgccagcca
      481 gaaggacaca taaaagaaga acattgcagc agaggcacag aaggagcctg cgaggagctg
      541 ggaaatacac acacaacagc agaaccacaa caccctcccc tggacacacc ctactgggga
      601 tcactgcttt tctttttttc tgaaccatcg cccacgccac acggagagaa atctctctct
      661 catcatcatc ctgaagaaaa cccccttatc ctcattttca cactgctgag gaaaacctac
      721 aatcgcacgg gctgagattt cctggcgaag actgtccttt ttcctttttc tttttttttc
      781 ttttcctttg gaaactgaca tttgcatttc tccattccaa gccacggcgt aataatatct
      841 gcaatccagc ctgaagacct gcaaatcgaa ggacctagat cactatcatc tttgtacgtc
      901 aaggatcctg gttactgtac gtataacctt tctttctttt gctctgacca atatgaaaca
      961 ctaaaatcga ttcgagcagc ctctcagcat caattacagt gcgtgaaaaa cattcaaata
     1021 gaggcagcaa atattacatg tgaaaataca ggctggctaa atccaagcta atttagaaat
     1081 gtggtcaaaa cgcatactgg cacgtctaat cgcggattca gtaaacaaga ttaacgatta
     1141 gcccagtgta taagtcatat gataccggca tgcgcgagag catcgctaca cccgagctgg
     1201 cttcattttc ggaggaaaat caaaacattg ctttctcctg ccgtgcgaac cattcgtcat
     1261 aaaccgtaat acgcaacata catttattac tacatccgtt aattagcgat aattagccgt
     1321 tattaacaaa gagcgctgag gaattcctct caaatagcgg aggtgcggcg gaggcagagg
     1381 ggcgtaaaag ggcacatgcg tgcctggctc aaaaaaggat gctccagact gaaactcaga
     1441 gacaaaacac gatgcctcgg aggctgagag cccatcgaga ggaaaggcaa agaaaagggt
     1501 cgagtcactc agagggggag gggagatatt gtgcatccat tggtttgaat tgaagcaggc
     1561 agaataaaaa tccttgcgat tatgcttcgt gagagatcgc gagagaggaa aaggctaaat
     1621 gccacgtatg caaatggata aatgtcatct atttcttcgc gagtagacat tttgtggaca
     1681 acgagagtgt aaataagcga tatgagggga atacaaaggc cttgagggaa agcgttttgg
     1741 gcgtgtcttg tcgaaaagaa gacagcgatg gcacgcgctc agaaccacca tcttcacgta
     1801 gacaatccgc gtgaaaacaa taacaaaact gctcatttta acacgagcag gtgtttgaaa
     1861 ccacccaaaa aagattgtct aaaatattaa gattaattaa tgacgtttaa atgtccaggc
     1921 ctgatataaa tgaagcgtgt agtaagagta atcgcctttg tttcttttca gagatacgtt
     1981 ttcagagatt cagggaatta tatcttcaat aaaataatgt ttgatttcat tattttaaag
     2041 ctttttaaga agtaaaaata cacattattc atttacattt tattttattt aaaggcctat
     2101 ggtcttgaaa ataataagtg gaatatatag gctatatatt cgtctaagga cagccggtgt
     2161 tattttatag tgacctcaga caaaagccga gacaaaacga ggcgcttgcc tttcatttta
     2221 atccttaata gacggtgtga tgaatgaatg aatgaatgaa taaatgaatg aatgacgggc
     2281 ccttcggcat gttgttccgc agtcctccgt gagcacgagt ctctaccgca gtgccaagca
     2341 cataaaaccg ttgagtcaga taaccttctc caggcgcgta tacacaattc aaggacaagc
     2401 acatcacaac tatacacaac acaacacaac cgcctaaacg cacgagctca tcaatcgcaa
     2461 tttaaaggca tgtcagtcaa agaaaaaggc ggcgttattt aaaaccaatg ttcaacaata
     2521 tttgtatgct gactagcccg ggcgcgcacg ctataagatc acaaggcctt gttttttccc
     2581 tttattcaaa atatcattaa ttattacagg tcataaaaca aaacaatgcg aattacaatg
     2641 atttaaagac tattgattta acaataatca gttaattata gaataatatt ataatatttt
     2701 acatacaaat gaaagatgac attatttgat gacattgtaa ttaaaatata tgcaatatat
     2761 ttttacgttt ttattaatta ggctattact attattataa ttatcattat ttttattatt
     2821 attattatta ttattaaata gtcttgcgaa ataagagtga aggcagttgc aatgttcatt
     2881 gttcccggag agtcccgcag cctctggcct caacacaaag cgctactgtt tacattaata
     2941 ttcatagtgc tgccatggtc tctgtagagg aggaacgggt ggctcttttg ttcgctagtg
     3001 tcaatacgtt tattcagcaa aaagcagctc ggatttgtga atgcccaaca agcacacgcg
     3061 cactccaatc aaaggacgag aggataacga ttttaaacga ttcgcaaacg tgtttatttg
     3121 taaagacgac atcaagagct taatgtccac ttgaaaagaa ataaatatgc ccgcggcgcc
     3181 aaggatattt tgaatgtagt cgttcatggt gagccactcc cgaattcagc actctggaga
     3241 gcgaccaaag aggaatagcg gaatagcaac tacttgagcc atttctctcc gtttgatggc
     3301 gttcatgatt aaaaatataa tcacgtgatt ttttatacat atataaatat atacataaag
     3361 taacgctttc cttttgagaa tacataaata tttattcgcg tgataaatca atgatcttat
     3421 atttatttgc acgaccgaaa ttagccaaat tcaccaatta aaaaaaaaat aagccaacaa
     3481 aaaagaaggg tttatccgtt caattttgac ttgtgtcctg ttgttttaca gctgccactg
     3541 tgatccctta agctgcagtg agagtgtggc taatgccttt tgtttaagat aatcctgtaa
     3601 tctgttaccg aaacggccta ttgacaagcc ggcattcaca tttcagtcag gaggcccgtc
     3661 cagacatgta catttatgaa ttacgaatga taaaattaag atctgcatta ggcgtcagaa
     3721 atgtcacgaa cacccattca tttcccacag ccaataatgg atcatgctgg gaagcgctat
     3781 cctcgcagtc tcaaaattaa tgttgacatg ttgcgtagag ctacagatta gatcagccat
     3841 atgctttatg tgtttacctc agcggatctt cagcaagatg tgtttatttt aagaaaatgg
     3901 cttcctcgct gcctgcacag gggcattaag gaagtgacgt gagcgtcctc acagtcaaat
     3961 tactttatct catgctgctt tcaggcctct gcttatttta ttattattat tttaaattag
     4021 ggggaatcac ggtggcgttg tgggtagggt agcgcgatca ccttacagca agaaggtcgc
     4081 tggttcgagc tctggctggg tcagttggca tttctgtgtg gagtttaaat tgaattgaaa
     4141 ttgaataaac taaattggcc ctagtgtatg tgtgtgaatg taagtgtgca tgggtgtttc
     4201 ccagtgttgg gtttgactgg aagagcatcc gcttcgtaaa acatatgctg gataagttgg
     4261 cggttcattt cactgtggtg accgctgatt aataaaggga ctaagctgaa aatgaaatga
     4321 attaatacat tttaaatgag cttttgctga catgtttatg attacaaata gtgaaattgt
     4381 atgttaattt acgataagtg cattgttcta aattcatttt tatgtagcag ctggtttttc
     4441 ttccatttgt gtcattcaac agaacatttt tctaaaaaag tgaacttaaa ttttctcctc
     4501 cctctgacaa ggcttgatgg taatttcagc cgactctaat tacagactca ctaaaaggaa
     4561 tcggtgcagt catttatctt tatcagcgcg ctggtgggaa ggtaataggt tttgcttatg
     4621 agttgtttgc gtgcgagtct gggtccttgt gtaggcgatg ctgatgcact gcattaaata
     4681 ttgagggaga agtcgctcaa aaatagcaaa gtacaggctg gggcaggaac gtcaaagccc
     4741 gctcagtggc cacgtttctt gtttcgtaag tcctattttt aatagcgata attcaggctc
     4801 agtagaagga actccagcac tataagcagt ctctcgcctg tctcggcgag ttatgtattt
     4861 tgggatgaag tgctcttcca cctctacctc tgctgtgctg gcgccagtat ctgggctgta
     4921 agatagtgta cgtgtcagtc tgtgtggccg aagagcctgt gtgttgtgta actcactaca
     4981 ttcgtcgctg tggatggctg acttgccatg gggaggttta cttcagttca cgaatcacat
     5041 tcgccacgat cttgtcactt tgtgtcaaga ctcgctctct gtgtgtcaac agctctgctg
     5101 tttgaggttg aaattctgaa cttgagatat atcccaggat atattagggc tactgccaac
     5161 ggaaaaagca aatggtgcca tggccatttg attgatcaaa caaacaccct tttgcttggg
     5221 tgacagtgaa atgtcagatc catgtttgta ctgctttaat aacttgcctc tctgagcttt
     5281 ctccttgtca gtgtcaattt gcctgagata taaattccgg ttgccccgaa agcttatttt
     5341 attaaaaaaa agctaacatg cactcataca acgtgcgcac atacatgtga ggcttgctaa
     5401 aagtcaagaa gcaagccaat agcaagagtc gtcagactgc tgttacctgg gcaacctggt
     5461 tagaggtaag gctcaaggca agaataaatc attccactga agagacacag cgttacagcg
     5521 agatgagagc aagaggcgat agtgaaggga gaacaaaaga acagcctacc cacactagtg
     5581 cagatctacc tgtttgagct actgattgtt cagtttgatt agatatcaga caattctgct
     5641 atctccaatt gcattgctgc atgaattcct agaaatcatt ttaatcacca aaaactagta
     5701 gggacacttg tggcgtatat gattctgcct gttgattgtg gggatccaac agaaagatca
     5761 tgaatgcatt gttaattaag tcggtgagac accctgttcc accctagggg ccgttctggg
     5821 gaaagagtgc tttcagtcag ctaaagatga ctaatatgtg aacatatatt actacacatg
     5881 ccaatttgta cattctggat agttaccagc cctgggaaaa catcgaaaaa aataaatcaa
     5941 catagataaa attggcaaat cccttctggt acgatgcaga gtgcttgctc tgagaacttg
     6001 tgcattgtga cagaaagcga ccgttaaaaa ctgaaagtag catctacaac ctgcatacat
     6061 ctgttctttg aaaatcccct gtaggccaaa ttaagcatca cgctgtgctc cacagcaaca
     6121 cggatgaacc gaaaacccag acacaaacgc acacactttt cacataaaca aggtttagtt
     6181 atgctagcta tgctttttcg tcctcccttt gtcatcagcg gcgagtgacg taacacagtt
     6241 tgaccctgga cagcgtaaag ggggggttgg gaatgagtga aaggactgtg acatctgcag
     6301 ggccaggggc tgatggagag agctgaagtg agggaggtca gaggaggagg aagggtggga
     6361 tattgtcctc ctcaggccct atccctctca gacgcattga tttttaacct ctcagcgaga
     6421 atgccaagca ttacacccct caaattatgg ttcctcaccc aacctgttat attttccacc
     6481 tgcagataat taccggtatg ctgcagaacg agcttgctct taagttggct ggacttgata
     6541 ttaacaagac tggaggttct catcatcacc accaccatgg atccatggtg agtgcgatta
     6601 agccagacat gaagatcaaa ctgcgtatgg aaggcaacgt aaacgggcac cactttgtga
     6661 tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca
     6721 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc ctgtacggca
     6781 acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
     6841 aggggtattc gtgggaacga agcatgactt tcgaagacgg gggcatttgc tatgccagaa
     6901 gcgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
     6961 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     7021 aaatgtatgt gcgtgatgga gtgctgacgg gtgatgtaga gatggctttg ttgcttgaag
     7081 gaaatgccca ttaccgagcg ttgtcaagcc gccgcaagtt caacaaaacc ggccatgcct
     7141 tgcgtgcaat tggccgcctg tccagcggag gctctggcgg ttccggcggt tcaggtggat
     7201 cagaccagct gaccgaggag caaattgcag aatttaagga ggcgttctct ttgtttgaca
     7261 aagatggcga cggtacaatt acaaccaagg aattgggcac agtaatgcgc tcccttggac
     7321 agaatcccac tgaagccgaa cttcaagaca tgatcaatga ggttgatgct gacggggatg
     7381 ggactatcga ctttcctgag tttcttacca tgatggctcg caaaatgaag gatacagaca
     7441 gtgaggagga aattcgtgag gcctttcgtg tgtttgataa ggacgggaac ggttatatct
     7501 cagcggcgga gctgcgtcac gttatgacca acttaggcga aaagctgact gatgaagaag
     7561 tcgatgagat gatccgcgag gcggacatcg atggtgatgg tcaagtcaat tatgaagagt
     7621 ttgtggtgat gatgaccgcg aagtgcctct tcagaactac ttacaaagct aaggagaagg
     7681 gtgtcaagtt accaggcgtg cactatgtgg accacaccat tgagatttta agccatgaca
     7741 aagattacaa caaggttaag atctatgagt atgctgttgc tcattctgga ttgcctgaca
     7801 atgccagacg aggaggtagc gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta
     7861 tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag
     7921 aaggcagacc gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag ggaggacccc
     7981 tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt acctttatca
     8041 agtacccggc cgacatccct gatttcttta aacagtcctt tcctgagggt tttacttggg
     8101 aaagagttac gagatacgaa gatggtggag tcgtcaccgt cacgcaggac accagccttg
     8161 aggatggcga gctcgtctac aacgtcaagg tcagaggggt aaactttccc tccaatggtc
     8221 ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag
     8281 atggtggtct gagaggatac actgacatcg cactgaaagt tgatggtggt ggccatctgc
     8341 actgcaactt cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg
     8401 gtgtccatgc cgttgatcac cgcctggaaa ggatcgagga gagtgacaat gaaacctacg
     8461 tagtgcaaag agaagtggca gttgccaaat acagcaacct tggtggtggc atggacgagc
     8521 tgtacaagta accggtggat cctggccgtc gttttactgt accatcgatg atgatccaga
     8581 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     8641 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     8701 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     8761 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     8821 tcagatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
     8881 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
     8941 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
     9001 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
     9061 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
     9121 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
     9181 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     9241 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     9301 aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta
     9361 ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     9421 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     9481 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9541 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9601 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9661 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9841 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10501 tggaacgaaa actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc
    10561 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10741 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
    11641 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    11701 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    11761 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    11821 ccgtctatca gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct
    11881 acccacgtac caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc
    11941 ctctttatag gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct
    12001 acctgttgga cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt
    12061 gaatatctgt tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg
    12121 agttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac
    12181 ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa
    12241 aagtaaaaaa gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca
    12301 agaaagaaaa ctagagattc ttgtttaagc ttcccgggtg tcgacgaatt ctcgagctct
    12361 attcctaaag accttgggtg accaaaatct tattttaata aataaaactg tttattaaaa
    12421 cttttttgtt tcaaagaacc atatgtatag tgaaatttat aaaaatatca atttttaaaa
    12481 agctggtgta ctcatttatg ttatgaactc taaaaccata tactgactgc aagtgatgat
    12541 gtatagagtg atgtttacga gtaaacatat ttagttgtat acatcctact gagcacattt
    12601 tgatgtatga aataacatta caagctttat ccaaattaag ccattttaaa acactgccaa
    12661 ttgaaaatac aaatcctgga aaaaatcgtc tttagcgcag tcatttgagc catcctaatc
    12721 cgttacctca gaccataata agaagggata acactagctg tagcaatgga acacatctgt
    12781 ttcacacaat catatctcct gcgccggtgc taagcagatt cagcgtgatc ataacatgct
    12841 ttccactcat aaatgtaaat ttacaatttg cacatgtaaa acagacactt ttgagatatt
    12901 ggataaaaaa acaagagtat attgcttagt ttcatccacc agtcatcccc acagcgtttg
    12961 gaaggccata aaaagtgtct aaaatcaatg atcattgaaa gagcacaaga gagactctta
    13021 cgctgtaatg ccactgggga caaaagtgac agtctcttaa tgggctcttc tggaggggct
    13081 cctgaacatt aaaaattatc agcgaaatta ccgaaagagc ttcaagcaac tggcatgctt
    13141 gatcctctgc gtcggggcgg tgaataggtg cttcagatgc cctcttaccc acgggctgga
    13201 ttcagctgcc ccgctaccag cggagacccc ctaatgagcc tctgcaatta agtttattca
    13261 tgttaagtgt gaacggggtg cgtgcggaac tgtgggcagc taacagacct gggttctttg
    13321 tgccacaagt gctgccttta ttcggctcac aaagcagaaa acaacacccg cacctataat
    13381 ggcgccctcg gctgggtcta agaaacgtgg cgagttgaca gagcagagtg ggcggggtta
    13441 agacagactg acagcgggac ccatctccat cctcttatta acgcttaacg agtgccttcc
    13501 tcatgcaata ttcatcgcca ctaatatcat ccaagctctg agctgagctg gccacttatg
    13561 taaggcaatt atgtaaaata tcagacaggg cccacactca gaatctgact ggggtagaga
    13621 cgcgggacga gaaccgagag caagaactga aagtgaaagt gaccactaaa gggaggagag
    13681 gacagagggg caggatgtgt caagattacc agagaacact tggccagaaa tgcgcaacca
    13741 ttggagctct ccggattacc caaaggttaa cgagtttgaa cgcctctgcc cactcgccca
    13801 tctctgatgg tttcccaaga actcctcaag caaaatatat ataattgtgt gtattatgca
    13861 cagacacgag aaaatgctgt ttttctgatc tgcattacag cacatttgcc cgccaacgac
    13921 aataccaccc actcggtacc tcgctgactc ctgatgcctg atacctgcgc ggtgactgtc
    13981 tacaatctgc ataatcaaga gaagttgtgt tgaagacgag cgccacacaa ccgtttccac
    14041 aaggtcaccc aaggccggtg cagatgtagg tgaggtctcc ataaacagac tgaaataaac
    14101 acatcctccg ctgggaacaa caaccccctc acgcctcatg catttccata agcctacatg
    14161 catctcttcc aacttatgga gactcgcacc taccaacatc cgcacaacaa agatatacag
    14221 agcgcgctcc ctcaggtcaa ggccctgtgg gggtctgtgc agaaataggt catttgtcac
    14281 acatcaagtc ctggggcagg agatgcatta tagatgagac caaacagcct gtctcggtga
    14341 gctctaccca ctccctgaga ctagaaatgg gggaagggag cttgagataa caaccgctgc
    14401 aatcactgtg tcgatgttta atatcagcac caaccgggaa caataaggag atgggtgcat
    14461 tcatgttcac atcttaccag tcaagtatca tcgaaccggc ttgataacca cacctcgtgt
    14521 aatagctgag cagatagttg tcattttaaa gcgttggcct ttgtcgatta tgtaatgcgc
    14581 a
//
