LOCUS       sequence-23781-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Lee K, Deng X, Friedman E
  TITLE     Mirk protein kinase is a mitogen-activated protein kinase substrate 
            that mediates survival of colon cancer cells.
  JOURNAL   Cancer Res. 2000 Jul 1. 60(13):3631-7.
  PUBMED    10910078
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2000 Jul 1. 60(13):3631-7."
COMMENT     Sequence Label: pcDNA-Mirk (pHX9F)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             154..186
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(190..198,199..213)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cgtggggnnt tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttacc
      121 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
      181 atgggtcggg atctgtacga cgatgacgat aaggtaccta ggatccagtg tggtggaatt
      241 ctgcagatcc tcgctcccct gcggccctct gagcccacca tggccgtccc accgggccat
      301 ggtcccttct ctggcttccc agggccccag gagcacacgc aggtattgcc tgatgtgcgg
      361 ctactgcctc ggaggctgcc cctggccttc cgggatgcaa cctcagcccc gctgcgtaag
      421 ctctctgtgg acctcatcaa gacctacaag cacatcaatg aggtatacta tgcgaagaag
      481 aagcggcggg cccagcaggc gccaccccag gattcgagca acaagaagga gaagaaggtc
      541 ctgaaccatg gttatgatga cgacaaccat gactacatcg tgcgcagtgg cgagcgctgg
      601 ctggagcgct acgaaattga ctcgctcatt ggcaaaggct cctttggcca ggtggtgaaa
      661 gcctatgatc atcagaccca ggagcttgtg ctaaaaanaa aaaaaaaaaa aaanagtntt
      721 tgnnaaacna ngcccaaatt gagctgcggc tgctgganct gatganccag catgacacgg
      781 agatgantac tatatagtac acctgaa
//
