LOCUS       sequence-237811-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00119511
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(311..327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(311..327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(324..346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    335..351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(359..365,366..383,384..389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(542..559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(793..812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acttggtctc taatgcataa atgtgttatt aaaagagttt tttctgatgg ttcttctgtt
       61 aaaattccag ctcaaccagc tcaatctact actatttcta aacaagctgt ttgggtttat
      121 ggtaattgtc aaccatttcc agttgttttg aatgatggtt ctaaattgtc tggtgaaatt
      181 gaaactccag ctgttactcc agttgaatct gttgatgaag atgattgttt tgaattggat
      241 atttctggtc aaagatgagc ttagagacca agttccatcc caatggcgcg ccgagcttgg
      301 ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      901 tttctcatag ctcacgctg
//
