LOCUS       sequence-237815-        9864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti sgRNA cloning backbone with CMV-puro resistance marker. 
            Contains BsmBI sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W
  TITLE     Genome-wide screening for functional long noncoding RNAs in human 
            cells by Cas9 targeting of splice sites.
  JOURNAL   Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283.
  PUBMED    30395134
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283."
COMMENT     Sequence Label: pLenti-sgRNA(lib)-puro
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..2889
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2641..2661
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2812..2831
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     2913..2932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2913..2931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2961..3085
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3514..3819)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3616..3635)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4139..4822)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4737..4756
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4823..4925)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5103..5227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     5355..5371
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5413..5446)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5562..5865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5866..6069
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6098..6697
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6098..6117)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6594..6614
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6753..7341
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6806..6826)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7224..7235)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7344..7360)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7870..8050
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8112..8700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8192..8211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8871..9662,9663..9731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9494..9513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9732..9836)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag gtaccaaggt ctggcaggaa
     2641 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     2701 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     2761 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     2821 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2881 cgaaacaccg agagacgctg gcttatcgaa attaatacga ctcactatag ggagacccaa
     2941 gctggctagt taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3001 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3061 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3121 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3181 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3241 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3301 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3361 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3421 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3481 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3541 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3601 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3661 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3721 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3781 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     3841 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     3901 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     3961 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4021 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4081 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4141 agtaagccgg atccacgcgg cgtttacgcc ccccctgcca ctcatcgcag tactgttgta
     4201 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     4261 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     4321 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     4381 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     4441 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     4501 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     4561 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     4621 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     4681 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     4741 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     4801 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4861 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4921 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4981 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     5041 cgtcgggtga tgctgccaac ttagtcgact acaggtcact aataccatct aagtagttga
     5101 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5161 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5221 aagtggttga tctagagggc ccgcggttcg aacgtctctg tttaagagct atgctggaaa
     5281 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     5341 ggtgcttttt ttctcgaggt cgacggtatc gataagctcg cttcacgaga ttccagcagg
     5401 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccaa
     5461 gcttaagcgg ccgcgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     5521 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5581 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5641 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5701 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     5761 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     5821 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     5881 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     5941 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6001 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6061 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg accgagtaca agcccacggt
     6121 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6181 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6241 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6301 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6361 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6421 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6481 cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6541 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6601 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6661 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaca tgtttaaggg ttccggttcc
     6721 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6781 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6841 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6901 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6961 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7021 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7081 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7141 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7201 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7261 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7321 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7381 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7441 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7501 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7561 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7621 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7681 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7741 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7801 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7861 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7921 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7981 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8041 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8881 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8941 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9001 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9061 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9121 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9181 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9241 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9301 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9361 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9421 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9481 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9541 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9601 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9661 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9721 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9781 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9841 tttccccgaa aagtgccacc tgac
//
