LOCUS       sequence-237828-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00125251
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(675..691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(675..691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(688..710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    699..715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(723..729,730..747,748..753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    768..789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttggtctcta atgggttgga ctggtaaatc tgaagcttct aatagaccac caccaggtag
       61 gagaccagaa gaaactactt ggtttacttc tgatccatct tatgcttgtg gtgttaaaat
      121 ttttagacca ggttctgaag gttcttctgg tttggctgaa attactcatt tgaattttca
      181 tgaaaaaatt aaagttttga aaggtcaata tgaatgtgaa gttggtccat cttgttctac
      241 tttgagatgt actatgccaa gaggttggag attggatatt tttggtaaat cttttccaac
      301 tagaagaact acttattttg ctgaaaatgg taatccagaa tataatactt tggcttctga
      361 agctaatatt ccattgtatg attgtagagt tgttattaga tttccaccaa ctggtggtag
      421 agatactggt ccattggctg aagctactca ttttaatttg ggtgaagttg ttggtgtttt
      481 gggtggtaga catacttgtc aagctcaagg tcaatgtgct aatgttaatt gtaatgttcc
      541 aaaaggttat acttattatt ctttgactgg tggtccattt caaactaaag aaaaatctca
      601 tacttttcaa tctgatgctt gagcttagag accaagttcc atcccaatgg cgcgccgagc
      661 ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      721 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      781 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      841 ctgcattaat ga
//
