LOCUS       sequence-237836-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00003601
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(379..395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(379..395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(392..414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    403..419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(427..433,434..451,452..457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    472..493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(610..627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttggtctct aatgcaacca gaatattgtt ttgttagaat tttgaaatat gaaattccaa
       61 ttcatggtga tggttatttg gttggttctg atgttaattg gtgggttggt aatcaaaaag
      121 ttgattttca tattaataat gattgtacta ttcaaagaag aggtggtact caagaaaaaa
      181 aaccatatag attggattct atttctactt tgggtgaaga tttgccaaaa ggttttgatt
      241 ttattttgtt tagagaatct aaaattgatg aatttttggc tcaaggtggt gttactaaaa
      301 gaattttggg ttctatgatt ttgtgagctt agagaccaag ttccatccca atggcgcgcc
      361 gagcttggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      421 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      481 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      541 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      721 catgtgagca aaagg
//
