LOCUS       sequence-237840-        4044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TaNAM-A1 CDS in the Gateway-compatible pCR8 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TaNAM-A1
FEATURES             Location/Qualifiers
     source          1..4044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1923..2022)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2039..2058)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2040..2058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2063..2079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2063..2079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2510..3301
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3394..3982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3883..3902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttatgaggtc catgggcagc tccgactcat cctccggctc
      721 ggcgcaaaaa gcagcgcggc atcagcatga gccgccgcct ccgcggcagc ggggctcggc
      781 gccggagctc ccaccgggct tccggttcca cccgacggac gaggagctgg tcgtgcacta
      841 cctcaagaag aaggccgcca aggtgccgct ccccgtcacc atcatcgccg aggtggatct
      901 ctacaagttc gacccatggg agctccccga gaaggcgacc ttcggggagc aggagtggta
      961 cttcttcagc ccgcgcgacc gcaagtaccc caacggcgcg cggccgaacc gggcggcgac
     1021 gtcgggctac tggaaggcca ccggcacgga caaacctatc ctggcctcgg ggacggggtg
     1081 cggcctggtc cgggagaagc tcggcgtcaa gaaggcgctc gtcttctacc gcgggaagcc
     1141 gcccaagggc ctcaaaacca actggatcat gcacgagtac cgcctcaccg acgcgtctgg
     1201 ctccaccacc accagccggc cgccgccgcc tgtgaccggc gggagccggg ctgcagcctc
     1261 tctgaggttg gacgactggg tgctgtgccg catctacaag aagatcaaca aggccgcggc
     1321 cggagatcag cagaggagca cggagtgcga ggactccgtg gaggacgcgg tcaccgcgta
     1381 cccgctctat gccacggcgg gcatggccgg tgcaggtgcg catggcagca actacgcttc
     1441 accttcactg ctccatcatc aggacagcca tttcctggag ggcctgttca cagcagacga
     1501 cgccggcctc tcggcgggcg ccacctcgct gagccacctg gccgcggcgg cgagggcgag
     1561 cccggctccg accaaacagt ttctcgcccc gtcgtcttca accccgttca actggctcga
     1621 tgcgtcaccc gccggcatcc tgccacaggc aaggaatttc cctgggttta acaggagcag
     1681 aaacgtcggc aatatgtcgc tgtcatcgac ggccgacatg gctggcgcgg ccggcaatgc
     1741 ggtgaacgcc atgtccgcat ttatgaatcc tctccccgtg caagacggga cgtaccatca
     1801 acaccatgtc atcctcggcg ccccactggc gccagaggct accacaggcg gcgccacctc
     1861 tggtttccag catcccgtcc aagtatccgg cgtgaactgg aatccctgaa agggcgaatt
     1921 cgacccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat caatttgttg
     1981 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2041 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2101 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2161 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     2221 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2281 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2341 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2401 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2461 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2521 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2581 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2641 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2701 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2761 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2821 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2881 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2941 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3001 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3061 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3121 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3181 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3241 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3301 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3541 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3781 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4021 ttgctggcct tttgctcaca tgtt
//
