LOCUS       sequence-237843-        4035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TtNAM-B1 CDS in the Gateway-compatible pCR8 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TtNAM-B1
FEATURES             Location/Qualifiers
     source          1..4035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1914..2013)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2030..2049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2031..2049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2054..2070)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2054..2070)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2501..3292
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3385..3973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3874..3893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttatgggcag ctccgactca tcttccggct cggcgcaaaa
      721 agcaacgcgg tatcaccatc agcatcagcc gccgcctccg cagcggggct cggcgccgga
      781 gctcccgccg ggcttccggt tccacccgac ggacgaggag ctggtggtgc actacctcaa
      841 gaagaaggcc gacaaggcgc cgctccccgt caacatcatc gccgaggtgg atctctacaa
      901 gttcgaccca tgggagctcc ccgagaaggc gaccatcggg gagcaggagt ggtacttctt
      961 cagcccgcgc gaccgcaagt accccaacgg cgcgcggccg aaccgggcgg cgacgtcggg
     1021 gtactggaag gccaccggca cggacaagcc tatcctggcc tcggggacgg ggtgcggcct
     1081 ggtccgggag aagctcggcg tcaagaaggc gctcgtgttc taccgcggga agccgcccaa
     1141 gggcctcaaa accaactgga tcatgcacga gtaccgcctc accgacgcat ctggctccac
     1201 caccgccacc aaccgaccgc cgccggtgac cggcgggagc agggctgctg cctctctcag
     1261 gttggacgac tgggtgctgt gccgcatcta caagaagatc aacaaggccg cggccggcga
     1321 tcagcagagg aacacggagt gcgaggactc cgtggaggac gcggtcaccg cgtacccgct
     1381 ctatgccacg gcgggcatga ccggtgcagg tgcgcatggc agcaactacg cttcaccttc
     1441 actgctccat catcaggaca gccatttcct ggacggcctg ttcacagcag acgacgccgg
     1501 cctctcggcg ggcgccacct cgctgagcca cctagcagcg gcggcgaggg caagcccggc
     1561 tccgaccaaa cagtttctcg ccccgtcgtc ttcgaccccg ttcaactggc tcgatgcgtc
     1621 accagtcggc atcctcccac aggcaaggaa ttttcctggg tttaacagga gcagaaacgt
     1681 cggcaatatg tcgctgtcat cgacggccga catggctggc gcagtggaca acggtggagg
     1741 caatgcggtg aacgccatgt ctacctatct tcccgtgcaa gacgggacgt accatcagca
     1801 gcatgtcatc ctcggcgctc cgctggtgcc agaagccgcc gccgccacct ctggattcca
     1861 gcatcccgtt caaatatccg gcgtgaactg gaatccctga aagggcgaat tcgacccagc
     1921 tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt gcaacgaaca
     1981 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2041 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2101 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     2161 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     2221 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     2281 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2341 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     2401 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     2461 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     2521 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2581 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     2641 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     2701 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2761 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2821 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     2881 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     2941 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3001 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3061 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3121 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3181 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3241 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     3301 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3361 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3421 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3481 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     3541 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3601 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3661 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3721 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     3781 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3841 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3901 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3961 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4021 ttttgctcac atgtt
//
