LOCUS       sequence-237848-        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TtNAM-B1(1-215)
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1344..1443)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1460..1479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1461..1479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1484..1500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1484..1500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1931..2722
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2815..3403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3304..3323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttatgggcag ctccgactca tcttccggct cggcgcaaaa
      721 agcaacgcgg tatcaccatc agcatcagcc gccgcctccg cagcggggct cggcgccgga
      781 gctcccgccg ggcttccggt tccacccgac ggacgaggag ctggtggtgc actacctcaa
      841 gaagaaggcc gacaaggcgc cgctccccgt caacatcatc gccgaggtgg atctctacaa
      901 gttcgaccca tgggagctcc ccgagaaggc gaccatcggg gagcaggagt ggtacttctt
      961 cagcccgcgc gaccgcaagt accccaacgg cgcgcggccg aaccgggcgg cgacgtcggg
     1021 gtactggaag gccaccggca cggacaagcc tatcctggcc tcggggacgg ggtgcggcct
     1081 ggtccgggag aagctcggcg tcaagaaggc gctcgtgttc taccgcggga agccgcccaa
     1141 gggcctcaaa accaactgga tcatgcacga gtaccgcctc accgacgcat ctggctccac
     1201 caccgccacc aaccgaccgc cgccggtgac cggcgggagc agggctgctg cctctctcag
     1261 gttggacgac tgggtgctgt gccgcatcta caagaagatc aacaaggccg cggccggcga
     1321 tcagcagtga aagggcgaat tcgacccagc tttcttgtac aaagttggca ttataaaaaa
     1381 taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     1441 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     1501 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1561 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
     1621 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     1681 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     1741 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1801 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     1861 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1921 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     1981 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2041 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     2101 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2161 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2221 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2281 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2341 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2401 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2461 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2521 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2581 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2641 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     2701 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     2761 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2941 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3181 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     3241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
