LOCUS       sequence-237857-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00040381
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(331..347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(331..347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(344..366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    355..371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(379..385,386..403,404..409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(562..579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttggtctct aagctcaagc acaaccaaca aaaccatctt gaccattaga tttacaacca
       61 ccagcaccag aagcagtagc aataacattt ctaccaattt taaaattatc aattggatca
      121 caaccaccca aaaaaccttt accacctttt ggtctatcag ctttaaaatt agaacaacaa
      181 aaagcatcca atttcatttt tttacaagtt tcacctggat cagtagtacc ttgaccacaa
      241 catctcaaac cagtttctgg agtagcagaa ttaaattgca ttagagacca agttccatcc
      301 caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta
      361 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      421 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      481 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      541 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      721 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      781 aagtcagagg tggcgaaacc cgacaggact ataaagatac c
//
