LOCUS       sequence-237861-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00090231
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(522..538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(535..557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    546..562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(570..576,577..594,595..600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    615..636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atggctaaat tgttgccaac taaaggtcat ggtgctccag atgtttgttt
       61 gggtgctatg tataaaaaat ctgaaggtgt taaaggtcca tctgctgtta gatttgttgc
      121 ttatccaggt ggtggtgatt cttttaaaca tggtggtaaa tggattactg ttatggttac
      181 tgatgattgt catttgagaa aaactccaag agaaactcca gaaggttata cttgggaaac
      241 taaagctatg gcttggtctg ataaaccatc tagagaagtt gttgttgaaa gagtttgggc
      301 tattgatgat gttatgttta atagacaaaa taaaccaggt caattggatt ctagatttag
      361 aggttttgat gaaaatggta aaatgttgcc aatgccaact agaggtattt tgtttacttg
      421 taaagttgat ttgtctttgg ctattgatgt tgttgaatgt gatgattgag cttagagacc
      481 aagttccatc ccaatggcgc gccgagcttg gctcgagcat ggtcatagct gtttcctgtg
      541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tc
//
