LOCUS       sequence-237865-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00101921
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(381..397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(381..397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(394..416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    405..421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(429..435,436..453,454..459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    474..495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(612..629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgcaaaaaa ttactgtttg tcaagttgaa ttgtggggtg ctgattctac
       61 taaaccatct gatactgctt gggtttctcc aggtggtatt gctagattta ctgttaaatt
      121 tggtagacat tctttgttta tttatgctaa aacttctgat gattgtactc catctccagt
      181 tttggaaggt ccatctagat ggggtactca tactattaga ggttctttgc aagaaaaaca
      241 aaaaggtact gttttggttc aagaagtttc tatgaaagaa gctactagag gttatccatc
      301 tggtttgaat ttgtcttctt ttatgtgagc ttagagacca agttccatcc caatggcgcg
      361 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      841 tggcgaaacc cgacaggact ataaagatac ca
//
