LOCUS       sequence-237873-        2726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human SOX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-60: SOX2-mEGFP
FEATURES             Location/Qualifiers
     source          1..2726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1013..1015,1016..1729)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1055..1076)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1316..1335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1663..1684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccgagggtc ggcggccgcc ggcgggccgg gcccgcgcac agcgcccgca tgtacaacat
       61 gatggagacg gagctgaagc cgccgggccc gcagcaaact tcggggggcg gcggcggcaa
      121 ctccaccgcg gcggcggccg gcggcaacca gaaaaacagc ccggaccgcg tcaagcggcc
      181 catgaatgcc ttcatggtgt ggtcccgcgg gcagcggcgc aagatggccc aggagaaccc
      241 caagatgcac aactcggaga tcagcaagcg cctgggcgcc gagtggaaac ttttgtcgga
      301 gacggagaag cggccgttca tcgacgaggc taagcggctg cgagcgctgc acatgaagga
      361 gcacccggat tataaatacc ggccccggcg gaaaaccaag acgctcatga agaaggataa
      421 gtacacgctg cccggcgggc tgctggcccc cggcggcaat agcatggcga gcggggtcgg
      481 ggtgggcgcc ggcctgggcg cgggcgtgaa ccagcgcatg gacagttacg cgcacatgaa
      541 cggctggagc aacggcagct acagcatgat gcaggaccag ctgggctacc cgcagcaccc
      601 gggcctcaat gcgcacggcg cagcgcagat gcagcccatg caccgctacg acgtgagcgc
      661 cctgcagtac aactccatga ccagctcgca gacctacatg aacggctcgc ccacctacag
      721 catgtcctac tcgcagcagg gcacccctgg catggctctt ggctccatgg gttcggtggt
      781 caagtccgag gccagctcca gcccccctgt ggttacctct tcctcccact ccagggcgcc
      841 ctgccaggcc ggggacctcc gggacatgat cagcatgtat ctccccggcg ccgaggtgcc
      901 ggaacccgcc gcccccagca gacttcacat gtcccagcac taccagagcg gcccggtgcc
      961 cggcacggcc attaacggca cactgcccct ctcacacatg ggcggaagcg gagtgagcaa
     1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1201 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     1261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1441 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     1501 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1621 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgag ggccggacag
     1741 cgaactggag gggggagaaa ttttcaaaga aaaacgaggg aaatgggagg ggtgcaaaag
     1801 aggagagtaa gaaacagcat ggagaaaacc cggtacgctc aaaaagaaaa aggaaaaaaa
     1861 aaaatcccat cacccacagc aaatgacagc tgcaaaagag aacaccaatc ccatccacac
     1921 tcacgcaaaa accgcgatgc cgacaagaaa acttttatga gagagatcct ggacttcttt
     1981 ttgggggact atttttgtac agagaaaacc tggggagggt ggggagggcg ggggaatgga
     2041 ccttgtatag atctggagga aagaaagcta cgaaaaactt tttaaaagtt ctagtggtac
     2101 ggtaggagct ttgcaggaag tttgcaaaag tctttaccaa taatatttag agctagtctc
     2161 caagcgacga aaaaaatgtt ttaatatttg caagcaactt ttgtacagta tttatcgaga
     2221 taaacatggc aatcaaaatg tccattgttt ataagctgag aatttgccaa tatttttcaa
     2281 ggagaggctt cttgctgaat tttgattctg cagctgaaat ttaggacagt tgcaaacgtg
     2341 aaaagaagaa aattattcaa atttggacat tttaattgtt taaaaattgt acaaaaggaa
     2401 aaaattagaa taagtactgg cgaaccatct ctgtggtctt gtttaaaaag ggcaaaagtt
     2461 ttagactgta ctaaatttta taacttactg ttaaaagcaa aaatggccat gcaggttgac
     2521 accgttggta atttataata gcttttgttc gatcccaact ttccattttg ttcagataaa
     2581 aaaaaccatg aaattactgt gtttgaaata ttttcttatg gtttgtaata tttctgtaaa
     2641 tttattgtga tattttaagg ttttcccccc tttattttcc gtagttgtat tttaaaagat
     2701 tcggctctgt attatttgaa tcagtc
//
