LOCUS       sequence-237875-        3930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-63: ACTN2-mEGFP
FEATURES             Location/Qualifiers
     source          1..3930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     325..344
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    343..398
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     444..463
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1687..1743)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2572..2628)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(2650..3360,3361..3363,3364..3366))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2692..2713)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3041..3060
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3300..3321
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     LTR             3400..3668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3681..3892)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gcatttaaat gttaacgatc tccataagag aagagggaca gctatgactg ggagtagtca
       61 ggagaggagg aaaaatctgg ctagtaaaac aggtaaggaa aattttaggg atgttaaaga
      121 aaaaaataac acaaaacaaa atataaaaaa aatctaacct caagtcaagg cttttctatg
      181 gaataaggaa tggacagcag ggggctgttt catatactga tgacctcttt atagccaacc
      241 tttgttcatg gcagccagca tatgggcata tgttgccaaa ctctaaacca aatactcatt
      301 ctgatgtttt aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt
      361 ccaacacact attgcaatga aaataaattt cctttattag ccagaagtca gatgctcaag
      421 gggcttcatg atgtccccat aatttttggc agagggaaaa agatctcagt ggtatttgtg
      481 agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggagt
      541 accggtctag ctagcttagt tggcctctcc catctccctg gcaaaggttc tggctaggtc
      601 acagatggtg gggatgctgc caggggtggt cacatgggtc tgcaccatgc cagaggttag
      661 ttgcagcaga gcatctctgc atccagcagg ggactggtca tagtccagga tgaacacatg
      721 catggacctc agcctcttgg ctagaacatc cagagcccag gcaaacacat tgtacaggtc
      781 tccattggga gccagcagct caggggctct gaacagggtg aacagggtgt ccccaatgtg
      841 gggtcttgga ccagcattgg actggggctc agctccttga gggggcactg ctgttccaga
      901 gagctgtccc cagtcctctc tccaagagcc tccacactgg aggtatctca cagtgtttgc
      961 cagcagtcca tacacccttc tgattgcagc cagcatggcc aggtccagtc tctctccagg
     1021 tctctgcctc tttgccagcc tgtcaatgtg tctgtcttca ggcagtgctc ccagaacaat
     1081 gttggtgcca gggagggttg gagggatcag ggccacaaaa gccagcacag cctgtggggt
     1141 catggagccc atgaggtacc ttgctgctgg gtagcacagc agggctgcaa tggggtgtct
     1201 gtcaaagatc agggtcaggg caggtggagg ggcatgagaa gagccagcct ctcctccaat
     1261 gtgaggagcc agaacagcat cagtcacagc atagggcatt cccattgtga tctgggcaga
     1321 ggtcatcacc acagcagcat ctccagcaga gatttctccc tggtccagcc tgtgctgggt
     1381 ggtgtagatg ttggcaatgg tctcagaggc tcctagcact ctccagtagg tcattggctc
     1441 aggcacatac acaatgtcat ccctggagcc cagtgccacc agcagttgag tggtggtggt
     1501 cttgcccatg ccatgaggtc catcaatgta caccctcagc agggttggca tcttctgctc
     1561 aggcctcacc tcagtggctt cctgctgtct tctgggtctt agggcagttc tcctgttgga
     1621 gtggcccctg gatctggcag cctggtcaaa ggcagaagca tgctggtgtc cagggtaaga
     1681 agccattggg cctgggttct cttcgacatc gccggcttgt ttgaggagag agaagtttgt
     1741 tgcgcctgag ccactagacc aatgcggagc atatacgccc cggagccgcg gcgatcctgc
     1801 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     1861 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     1921 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     1981 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     2041 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     2101 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     2161 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     2221 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     2281 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
     2341 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     2401 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     2461 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     2521 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatag taggtccagg
     2581 gttctcctcc acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttccggt
     2641 gctagcaccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     2701 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct
     2761 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     2821 gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt
     2881 gatgccgttc ttctgcttgt cggcggtgat atagacgttg tcgctgatgt agttgtactc
     2941 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     3001 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     3061 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     3121 gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc acgccccagg acagggtggt
     3181 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     3241 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     3301 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     3361 caccatggtg ggctcgaggt aggcgccggt cacagcttgg atctgtaacg gcgcagaaca
     3421 gaaaacgaaa caaagacgta gagttgagca agcagggtca ggcaaagcgt ggagagccgg
     3481 ctgagtctag gtaggctcca agggagcgcc ggacaaaggc ccggtctcga cctgagcttt
     3541 aaacttacct agacggcgga cgcagttcag gaggcaccac aggcgggagg cggcagaacg
     3601 cgactcaacc ggcgtggatg gcggcctcag gtagggcggc gggcgcgtga aggagagatg
     3661 cgagcccctc gaagcttcag ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc
     3721 acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag
     3781 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc cgttcaattg
     3841 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg
     3901 gcaccggagc gatcgcagat ccttgttaac
//
