LOCUS       sequence-237890-        1378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: N236H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pcDNA-PU.1-ETS N236H
FEATURES             Location/Qualifiers
     source          1..1378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(557..574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    563..790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     gap             1351..1362
                     /estimated_length=12
ORIGIN
        1 cnnngnnnng nnnnagcagn nntctctggn tactanagaa cccacnggct tnnnggctta
       61 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgctac cggtcgccac
      121 catgggatcg aaaaaaaaga tccgccttta ccaattctta ctggaccttc tgcgctctgg
      181 agatatgaaa gactcaattt ggtgggttga caaagacaaa ggcacttttc aattcagttc
      241 aaagcataag gaagctctgg cccatcgttg gggaattcaa aaaggtaacc gcaagaaaat
      301 gacttaccag aaaatggcgc gtgccttgcg tcattacgga aaaacagggg aggtgaagaa
      361 agtaaagaaa aaacttacat atcaattcag cggggaagtt ttaggacgcg gtggcttggc
      421 tgaacgccgt cacccacctc attgaaagct tggtaccgag ctcggatcca ctagtccagt
      481 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
      541 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg ngggggtggg
      721 ggcaggacag caagggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctaggggggt atccccacgc
      841 gccctgtagc ggcgcattaa gcgcggcggg ggntggnggn ttacgcgcag cgtgacccgc
      901 tacacttgcc cagcgcccct agcgccccgc tccttttcgc ttttcttccc ttccttttct
      961 cgccacgttt cgcccggctt ttccccgtca agctcttaaa tcggggggct cccttttagg
     1021 gttccnaatt tantgctttt acggcnaccn nnacccccaa aaaacttgga ttaagggtga
     1081 anggtttcac gttagtggnc cnatcncccc tgaatagaac ggttttttcg cccctttgaa
     1141 cgttggnant tcnncgtttc ttttaataag tggaactcct tggttccnaa actgggaaca
     1201 anacttcaac ccctattctc ggntcntatt tcttttngaa ttnaaanngg gnaattttnn
     1261 ccnaatttnc ggnccntaat tggtttaaaa aaatggannn tgaatttnaa caaaantttt
     1321 naancnnnna attnnanttn nnngttggaa nnnnnnnnnn nnaanttnaa ngggntgg
//
