LOCUS       sequence-237899-       10209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM
  TITLE     Angelman syndrome-associated point mutations in the Zn-binding 
            N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding 
            to the proteasome.
  JOURNAL   J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653.
  PUBMED    30257870
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653."
COMMENT     Sequence Label: PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)
FEATURES             Location/Qualifiers
     source          1..10209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2950..2974
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5370..5394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5413..5912
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5448..5471)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5845..5864
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5933..6532
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5933..5952)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6429..6449
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6599..7187
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6652..6672)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7070..7081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7262..7495
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7614..7632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7700..7804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7805..7873,7874..8665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8023..8042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8836..9424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9325..9344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9578..9595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9835..9856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9871..9876,9877..9894,9895..9901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9909..9925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9914..9936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9933..9949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9933..9949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9999..10018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcattaatg caaaactctg tgatcctcat
     3001 ccctccaaga aaggagcaag ctcagcttac cttgagaact cgaaaggtgc ccccaacaac
     3061 tcctgctctg agataaaaat gaacaagaaa ggcgctagaa ttgattttaa agatgtgact
     3121 tacttaacag aagagaaggt atatgaaatt cttgaattat gtagagaaag agaggattat
     3181 tcccctttaa tccgtgttat tggaagagtt ttttctagtg ctgaggcatt ggtacagagc
     3241 ttccggaaag ttaaacaaca caccaaggaa gaactgaaat ctcttcaagc aaaagatgaa
     3301 gacaaagatg aagatgaaaa ggaaaaagct gcatgttctg ctgctgctat ggaagaagac
     3361 tcagaagcat cttcctcaag gataggtgat agctcacagg gagacaacaa tttgcaaaaa
     3421 ttaggccctg atgatgtgtc tgtggatatt gatgccatta gaagggtcta caccagattg
     3481 ctctctaatg aaaaaattga aactgccttt ctcaatgcac ttgtatattt gtcacctaac
     3541 gtggaatgtg acttgacgta tcacaatgta tactctcgag atcctaatta tctgaatttg
     3601 ttcattatcg taatggagaa tagaaatctc cacagtcctg aatatctgga aatggctttg
     3661 ccattatttt gcaaagcgat gagcaagcta ccccttgcag cccaaggaaa actgatcaga
     3721 ctgtggtcta aatacaatgc agaccagatt cggagaatga tggagacatt tcagcaactt
     3781 attacttata aagtcataag caatgaattt aacagtcgaa atctagtgaa tgatgatgat
     3841 gccattgttg ctgcttcgaa gtgcttgaaa atggtttact atgcaaatgt agtgggaggg
     3901 gaagtggaca caaatcacaa tgaagaagat gatgaagagc ccatccctga gtccagcgag
     3961 ctgacacttc aggaactttt gggagaagaa agaagaaaca agaaaggtcc tcgagtggac
     4021 cccctggaaa ctgaacttgg tgttaaaacc ctggattgtc gaaaaccact tatccctttt
     4081 gaagagttta ttaatgaacc actgaatgag gttctagaaa tggataaaga ttatactttt
     4141 ttcaaagtag aaacagagaa caaattctct tttatgacat gtccctttat attgaatgct
     4201 gtcacaaaga atttgggatt atattatgac aatagaattc gcatgtacag tgaacgaaga
     4261 atcactgttc tctacagctt agttcaagga cagcagttga atccatattt gagactcaaa
     4321 gttagacgtg accatatcat agatgatgca cttgtccggc tagagatgat cgctatggaa
     4381 aatcctgcag acttgaagaa gcagttgtat gtggaatttg aaggagaaca aggagttgat
     4441 gagggaggtg tttccaaaga attttttcag ctggttgtgg aggaaatctt caatccagat
     4501 attggtatgt tcacatacga tgaatctaca aaattgtttt ggtttaatcc atcttctttt
     4561 gaaactgagg gtcagtttac tctgattggc atagtactgg gtctggctat ttacaataac
     4621 tgtatactgg atgtacattt tcccatggtt gtctacagga agctaatggg gaaaaaagga
     4681 acttttcgtg acttgggaga ctctcaccca gttctatatc agagtttaaa agatttattg
     4741 gagtatgaag ggaatgtgga agatgacatg atgatcactt tccagatatc acagacagat
     4801 ctttttggta acccaatgat gtatgatcta aaggaaaatg gtgataaaat tccaattaca
     4861 aatgaaaaca ggaaggaatt tgtcaatctt tattctgact acattctcaa taaatcagta
     4921 gaaaaacagt tcaaggcttt tcggagaggt tttcatatgg tgaccaatga atctccctta
     4981 aagtacttat tcagaccaga agaaattgaa ttgcttatat gtggaagccg gaatctagat
     5041 ttccaagcac tagaagaaac tacagaatat gacggtggct ataccaggga ctctgttctg
     5101 attagggagt tctgggaaat cgttcattca tttacagatg aacagaaaag actcttcttg
     5161 cagtttacaa cgggcacaga cagagcacct gtgggaggac taggaaaatt aaagatgatt
     5221 atagccaaaa atggcccaga cacagaaagg ttacctacat ctcatacttg ctttaatgtg
     5281 cttttacttc cggaatactc aagcaaagaa aaacttaaag agagattgtt gaaggccatc
     5341 acgtatgcca aaggatttgg catgctgtaa cccaactttc ttgtacaaag tggttcgata
     5401 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     5461 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     5521 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5581 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5641 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5701 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5761 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5821 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5881 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     5941 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6001 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6061 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6121 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6181 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6241 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6301 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6361 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6421 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6481 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6541 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tagatcctaa
     6601 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6661 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6721 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6781 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6841 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6901 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6961 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7021 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7081 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7141 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     7201 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7261 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7321 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7381 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7441 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7501 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7561 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7621 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8041 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9661 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9781 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10021 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10081 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10141 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10201 cttagtagt
//
