LOCUS       sequence-237901-        8463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mScarletI and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mScarletI-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    760..784
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      801..810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             807..1502
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(940..958)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1208..1227
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1509..1562
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1578..1587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1584..1586,1587..1589,1590..2303)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1629..1650)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1890..1909)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2237..2258
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2313..2337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2399..2418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2400..2418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2566..2753
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2621..2645)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2765..2782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2765..2781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2774..2796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3217..4563
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4674..5477)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5478..5693)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5718..5736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5804..5908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5909..5977,5978..6769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6127..6146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6940..7528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7429..7448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7682..7699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7816..7837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7852..7857,7858..7875,7876..7882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7890..7906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7895..7917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7914..7930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7914..7930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7960..8415)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8097..8118)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8309..8328
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggatcaccca tacatcactc tgttttgcct gaccttttcc ggtaatttga aaacaaaccc
       61 ggtctcgaag cggagatccg gcgataatta ccgcagaaat aaacccatac acgagacgta
      121 gaaccagccg cacatggccg gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg
      181 catctgtatt tcctaatgcg gcacttccag gcctcgatcg agaccgttta tccattgctt
      241 ttttgttgtc tttttccctc gttcacagaa agtctgaaga agctatagta gaactatgag
      301 ctttttttgt ttctgttttc cttttttttt tttttacctc tgtggaaatt gttactctca
      361 cactctttag ttcgtttgtt tgttttgttt attccaatta tgaccggtga cgaaacgtgg
      421 tcgatggtgg gtaccgctta tgctcccctc cattagtttc gattatataa aaaggccaaa
      481 tattgtatta ttttcaaatg tcctatcatt atcgtctaac atctaatttc tcttaaattt
      541 tttctctttc tttcctataa caccaatagt gaaaatcttt ttttcttcta tatctacaaa
      601 aacttttttt ttctatcaac ctcgttgata aattttttct ttaacaatcg ttaataatta
      661 attaattgga aaataaccat tttttctctc ttttatacac acattcaaaa gaaagaaaaa
      721 aaatataccc cagcctcgac tagtgaattc gcggccatca caagtttgta caaaaaagca
      781 ggctgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggcagtg atcaaggagt
      841 tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg
      901 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      961 gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg
     1021 ccttcatcaa gcaccccgcc gacatccccg actactataa gcagtccttc cccgagggct
     1081 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg acccaggaca
     1141 cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc
     1201 ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc gagcggttgt
     1261 accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg
     1321 gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg cagatgcccg
     1381 gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac tacaccgtgg
     1441 tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     1501 agtccggaga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     1561 ctggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg
     1801 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta ctttagcgac aacgtctata
     2041 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2101 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2281 gcatggacga gctgtacaag taaagcggcc gcacccagct ttcttgtaca aagtggtgta
     2341 gttaattcat gatggccgct gcaggtcgac ctcgaggggg ggcccggtac ccaattcgcc
     2401 ctatagtgag tcgtattacg cgcggatcca gctttggact tcttcgccag aggtttggtc
     2461 aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa
     2521 aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga
     2581 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     2641 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     2701 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcca
     2761 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2821 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2881 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2941 tccttacgca tctgtgcggt atttcacacc gcataatcgg atcgtacttg ttacccatca
     3001 ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct
     3061 gtataataat atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga
     3121 gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg
     3181 cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt
     3241 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     3301 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     3361 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     3421 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     3481 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     3541 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     3601 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     3661 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     3721 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     3781 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     3841 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     3901 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     3961 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     4021 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     4081 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     4141 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc
     4201 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
     4261 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
     4321 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
     4381 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
     4441 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
     4501 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
     4561 atcgatccga tgataagctg tcaaacatga gaattgggta ataactgata taattaaatt
     4621 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
     4681 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
     4741 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
     4801 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
     4861 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
     4921 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
     4981 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
     5041 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
     5101 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
     5161 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
     5221 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
     5281 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
     5341 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
     5401 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
     5461 tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg
     5521 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
     5581 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
     5641 tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctaa
     5701 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5761 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5821 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5881 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5941 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6001 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6061 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6121 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6181 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6241 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6301 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6361 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6421 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6481 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6541 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6601 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6661 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6721 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6781 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7621 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7681 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7741 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7801 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7861 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7921 cagctatgac catgattacg ccaagcttac cgcatcagga aattgtaagc gttaatattt
     7981 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8041 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8101 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     8161 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8221 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8281 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8341 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8401 cgctacaggg cgcgtccatt cgccaagctt cctgaaacgg agaaacataa acaggcattg
     8461 ctg
//
