LOCUS       sequence-237902-       14760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pmbfA::gRNA1(cexA)::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-710
FEATURES             Location/Qualifiers
     source          1..14760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             42..4145
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             42..4145
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             42..4145
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4158..4178
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4186..4959
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      5115..5124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5708..6733
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5708..6733
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     rep_origin      complement(10313..10901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10393..10412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     ncRNA           12494..12530
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        12551..12626
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           12631..12698
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      12714..13487
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
ORIGIN
        1 ttgccttgtc tgccgtcatt gcaacccacc caccaggaca catggacaag aagtactcca
       61 ttgggctcga tatcggcaca aacagcgtcg gttgggccgt cattacggac gagtacaagg
      121 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
      181 tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
      241 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
      301 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
      361 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
      421 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg
      481 ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac
      541 acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc
      601 aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg
      661 acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg
      721 cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg
      781 ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga
      841 gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg
      901 cagacctttt tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc
      961 gagtgaacac ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg
     1021 agcaccacca agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt
     1081 acaaggaaat tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag
     1141 caagccagga ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg
     1201 aggagctgct ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca
     1261 atggaagcat cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag
     1321 aggatttcta cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc
     1381 ggatacccta ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc
     1441 gcaaatcaga agagactatc actccctgga acttcgagga agtcgtggat aagggggcct
     1501 ctgcccagtc cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg
     1561 tgcttcctaa acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg
     1621 tcaaatacgt cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag
     1681 ctatcgtgga cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagagg
     1741 actatttcaa aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct
     1801 tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc
     1861 tggacaatga ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg
     1921 aagataggga gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag
     1981 tcatgaaaca gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga
     2041 tcaatgggat ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg
     2101 gatttgccaa ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg
     2161 acatccagaa agcacaagtt tctggccagg gggacagcct tcacgagcac atcgctaatc
     2221 ttgcaggtag cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac
     2281 tcgtcaaagt aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga
     2341 accaaactac ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg
     2401 gtataaaaga actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc
     2461 agaatgagaa gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg
     2521 aactggacat caatcggctc tccgactacg acgtggatca tatcgtgccc cagtcttttc
     2581 tcaaagatga ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga
     2641 gtgataacgt cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc
     2701 tgaacgccaa actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg
     2761 gcctgtctga gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga
     2821 tcaccaagca cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg
     2881 acaaactgat tcgagaggtg aaagttatta ctctgaagtc taagctcgtc tcagatttca
     2941 gaaaggactt tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg
     3001 cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg
     3061 aatttgttta cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc
     3121 aggaaatagg caaggccacc gctaagtact tcttttacag caatattatg aattttttca
     3181 agaccgagat tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg
     3241 gagaaacagg agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc
     3301 tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca
     3361 aggaaagtat cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg
     3421 accccaagaa atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg
     3481 ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca
     3541 caatcatgga gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat
     3601 ataaagaggt caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg
     3661 aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg
     3721 cactgccctc taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag
     3781 ggtcccccga agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg
     3841 atgagatcat cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc
     3901 tcgataaggt gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag
     3961 aaaacattat ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact
     4021 tcgacaccac catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac
     4081 tgattcatca gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg
     4141 gagacagcag ggctgacccc aagaagaaga ggaaggtgtg agcttgatcc acttaacgtt
     4201 actgaaatca tcaaacagct tgacgaatct ggatataaga tcgttggtgt cgatgtcagc
     4261 tccggagttg agacaaatgg tgttcaggat ctcgataaga tacgttcatt tgtccaagca
     4321 gcaaagagtg ccttctagtg atttaatagc tccatgtcaa caagaataaa acgcgtttcg
     4381 ggtttacctc ttccagatac agctcatctg caatgcatta atgcattgga cctcgcaacc
     4441 ctagtacgcc cttcaggctc cggcgaagca gaagaatagc ttagcagagt ctattttcat
     4501 tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga gtcctacgag actgaggaat
     4561 ccgctcttgg ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct
     4621 tctttactct gatagcttga ctatgaaaat tccgtcacca gcccctgggt tcgcaaagat
     4681 aattgcactg tttcttcctt gaactctcaa gcctacagga cacacattca tcgtaggtat
     4741 aaacctcgaa aatcattcct actaagatgg gtatacaata gtaaccatgc atggttgcct
     4801 agtgaatgct ccgtaacacc caatacgccg gccgaaactt ttttacaact ctcctatgag
     4861 tcgtttaccc agaatgcaca ggtacacttg tttagaggta atccttcttt ctagaagtcc
     4921 tcgtgtactg tgtaagcgcc cactccacat ctccactcgc taattcatga ataacggtga
     4981 gactagcggc cggtcccctt atcccagctg ttccacgttg gcctgcccct cagttagcgc
     5041 tcaactcaat gcccctcact ggcgaggcga gggcaaggat ggaggggcag catcgcctga
     5101 gttggagcaa agcggccgcc atgggagcag cgaaccaacg gagggatgcc gtgctttgtc
     5161 gtggctgctg tggccaatcc gggcccttgg ttggctcaca gagcgttgct gtgagtccat
     5221 gagctattat tgctaggtac agtatagaga gaggagagag agagagagag agagagggga
     5281 aaaaaggtga ggttgaagtg agaaaaaaaa aaaaaaaaat ccaaccactg acggctgccg
     5341 gctctgccac ccccctccct ccaccccaga ccacctgcac actcagcgcg cagcatcacc
     5401 taatcttggc tcgccttccc gcagctcagg ttgttttttt tttctctctc cctcgtcgaa
     5461 gccgcccttg ttcccttatt tatttccctc tccatccttg tctgcctttg gtccatctgc
     5521 ccctttgtct gcatctcttt tgcacgcatc gccttatcgt cgtctctttt ttcactcacg
     5581 ggagcttgac gatgacctga ctcgtgagcc tcacctgctg atttctctcc ccccctcccg
     5641 accggcttga cttttgtttc tcctccagta ccttatcgcg aagccggaag aacctcttaa
     5701 cctctagatg aaaaagcctg aactcaccgc gacgtctgtc gagaagttcc tgatcgaaaa
     5761 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5821 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5881 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5941 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     6001 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     6061 catggatgcg atcgctgcgg ccgatctcag ccagacgagc gggttcggcc cattcggacc
     6121 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     6181 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     6241 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     6301 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     6361 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6421 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcacccgg agcttgcagg
     6481 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6541 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6601 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6661 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6721 ggcaaaggaa tagatgcatg gctttcgtga ccgggcttca aacaatgatg tgcgatggtg
     6781 tggttcccgg ttggcggagt ctttgtctac tttggttgtc tgtcgcaggt cggtagaccg
     6841 caaatgagca actgatggat tgttgccagc gatactataa ttcacatgga tggtctttgt
     6901 cgatcagtag ctagtgagag agagagaaca tctatccaca atgtcgagtg tctattagac
     6961 atactccgag aataaagtca actgtgtctg tgatctaaag atcgattcgg cagtcgagta
     7021 gcgtataaca actccgagta ccagcgaaag cacgtcgtga caggagcagg gctttgccaa
     7081 ctgcgcaacc ttgcttgaat gaggatacac ggggtgcaac atggctgtac tgatccatcg
     7141 caaccaaaat ttctgtttat agatcaagct ggtagattcc aattactcca cctcttgcgc
     7201 ttctccatga catgtaagtg cacgtggaaa ccatacccaa attgcctaca gctgcggagc
     7261 atgagcctat ggcgatcagt ctggtcatgt taaccagcct gtgctctgac gttaatgcag
     7321 aatagcttat gctgcagacg cgttacggcg gagatgctgc agacgcgtta cgcggagatg
     7381 ctgcagacgc gttacgtatc ggatccagaa ttcgtgatat ctgaattcgt cgacaagctt
     7441 attttttgta tactgttttg tgatagcacg aagtttttcc acggtatctt gttaaaaata
     7501 tatatttgtg gcgggcttac ctacatcaaa ttaataagag actaattata aactaaacac
     7561 acaagcaagc tactttaggg taaaagttta taaatgcttt tgacgtataa acgttgcttg
     7621 tatttattat tacaattaaa ggtggataga aaacctagag actagttaga aactaatctc
     7681 aggtttgcgt taaactaaat cagagcccga gaggttaaca gaacctagaa ggggactaga
     7741 tatccgggta gggaaacaaa aaaaaaaaac aagacagcca catattaggg agactagtta
     7801 gaagctagtt ccaggactag gaaaataaaa gacaatgata ccacagtcta gttgacaact
     7861 agatagattc tagattgagg ccaaagtctc tgagatccag gttagttgca actaatacta
     7921 gttagtatct agtctcctat aactctgaag ctagaataac ttactactat tatcctcacc
     7981 actgttcagc tgcgcaaacg gagtgattgc aaggtgttca gagactagtt attgactagt
     8041 cagtgactag caataactaa caaggtatta acctaccatg tctgccatca ccctgcactt
     8101 cctcgggctc agcagccttt tcctcctcat tttcatgctc attttccttg tttaagactg
     8161 tgactagtca aagactagtc cagaaccaca aaggagaaat gtcttaccac tttcttcatt
     8221 gcttgtctct tttgcattat ccatgtctgc aactagttag agtctagtta gtgactagtc
     8281 cgacgaggac ttgcttgtct ccggattgtt ggaggaactc tccagggcct caagatccac
     8341 aacagagcct tctagatgac tggtcaataa ctagttggtc tttgtctgag tctgacttac
     8401 gaggttgcat actcgctccc tttgcctcgt caatcgatga gaaaaagcgc caaaactcgc
     8461 aatatggctt tgaaccacac ggtgctgaga ctagttagaa tctagtccca aactagcttg
     8521 gatagcttac ctttgccctt tgcgttgcga caggtcttgc agggtatggt tcctttctca
     8581 ccagctgatt tagctgcctt gctaccctca cggcggatct gcataaagag tggctagagg
     8641 ttataaatta gcactgatcc taggtacggg gctgaatgta acttgccttt cctttctcat
     8701 cgcgcggcaa gacaggcttg ctcaaattcc taccagtcac aggggtatgc acggcgtacg
     8761 gaccacttga actagtcaca gattagttag caactagtct gcattgaatg gctgtactta
     8821 cgggccctcg ccattgtcct gatcatttcc agcttcaccc tcgttgctgc aaagtagtta
     8881 gtgactagtc aaggactagt tgaaatggga gaagaaactc acgaattctc gacaccctta
     8941 gtattgtggt ccttggactt ggtgctgcta tatattagct aatacactag ttagactcac
     9001 agaaacttac gcagctcgct tgcgcttctt ggtaggagtc ggggttggga gaacagtgcc
     9061 ttcaaacaag ccttcatacc atgctacttg actagtcagg gactagtcac caagtaatct
     9121 agataggact tgcctttggc ctccatcagt tccttcatag tgggaggtcc attgtgcaat
     9181 gtaaactcca tgccgtggga gttcttgtcc ttcaagtgct tgaccaatat gtttctgttg
     9241 gcagagggaa cctgtcaact agttaataac tagtcagaaa ctagtatagc agtagactca
     9301 ctgtacgctt gaggcatccc ttcactcggc agtagacttc atatggatgg atatcaggca
     9361 cgccattgtc gtcctgtgga ctagtcagta actaggctta aagctagtcg ggtcggctta
     9421 ctatcttgaa atccggcagc gtaagctccc cgtccttaac tgcctcgaga tagtgacagt
     9481 actctgggga ctttcggaga tcgttatcgc gaatgctcgg catactaatc gttgactagt
     9541 cttggactag tcccgagcaa aaaggattgg aggaggagga ggaaggtgag agtgagacaa
     9601 agagcgaaat aagagcttca aaggctatct ctaagcagta tgaaggttaa gtatctagtt
     9661 cttgactaga tttaaaagag atttcgacta gttatgtacc tggagtttgg atataggaat
     9721 gtgttgtggt aacgaaatgt aagggggagg aaagaaaaag tcggtcaaga ggtaactcta
     9781 agtcggccat tcctttttgg gaggcgctaa ccataaacgg catggtcgac ttagagttag
     9841 ctcagggaat ttagggagtt atctgcgacc accgaggaac ggcggaatgc caaagaatcc
     9901 cgatggagct ctagctggcg gttgacaacc ccaccttttg gcgtttctgc ggcgttgcag
     9961 gcgggactgg atacttcgta gaaccagaaa ggcaaggcag aacgcgctca gcaagagtgt
    10021 tggaagtgat agcatgatgt gccttgttaa ctaggtcaaa atctgcagta tgcttgatgt
    10081 tatccaaagt gtgagagagg aaggtccaaa catacacgat tgggagaggg cctaggtata
    10141 agagtttttg agtagaacgc atgtgagccc agccatctcg aggagattaa acacgggccg
    10201 gcatttgatg gctatgttag taccccaatg gaaagcctga gagtccagtg gcgctgtgag
    10261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10921 ctacggggtg gagcgcaaaa aaccccgccc ctgacagggc ggggtttttt cgcgatcgga
    10981 ggtacagtgg ccatgaaatc caatcatttc cttctggccg ccctcgggca agagatagtg
    11041 ccgcagagga ctctcacagc atctacatct gcgaccgcaa cagccaccaa gcgaggcgca
    11101 catgagcttg tcctcctccc atgccaaagt ttggccctct tcgtttctgt gatgctgaag
    11161 gaagtcaaac tcgtcgatga taggaccaga tggtttgtca agggtcaacg ctttccatgc
    11221 cttctggcac cggtagtaat gctcttctgc aagggagact tgacgtttcg gatccgcggg
    11281 ccccgggaca tgctggaagg gattttctgg ctcaatacca cgtctgtatt tgaccctttc
    11341 cagacagtta atccgctgca ggagggcgaa ctgtagctcc tcgttctcct tgtagcgctt
    11401 gatccagtct ttttggatgt tgcacttgct tggcctatgc ttctcatata atcttgccct
    11461 gtcatagaga cgacgtctga gattgtagcg ttcgtctttg atcacccgga gccagatagg
    11521 cctgagtata tctgacatta gatcaaaggg tctgtggata gtctccttca gcatcagcga
    11581 cgcatgtgac tcgcatgtcg gagagagctt gtgggtggtc atctttgatg gcgtcctctg
    11641 ctttcccttg attttcgttg attgtttttc gaaagttaag tctggaagtc aagagaatcc
    11701 ttctgccaga cattatattt acgtatactg acgtagtaga aacagcgtca ggatgaggac
    11761 atggtgtgtg ctggaccacg gaatcatagt tcatcagtat attgggttgg acaaataacg
    11821 ctgagcatgt atatgtcttt acacactata aaagccagcg aacgccaata aaatagggca
    11881 tattgatgtg aaaatatgac accagttaaa agcagtgtat tgattttatc tctcttcacc
    11941 tcggacctat actaccgtat acaagactca acttacttcc agatatagta atatacaccc
    12001 tatggacgaa ccagcacaat aattacagcc aaacaacacc acccaaatgg catattccta
    12061 atcagcacta agcacaaata ccactgtcat cacagcataa tcaataagaa tcccagacaa
    12121 ccgactcact ctgactcacc ttacacaaac ccccaagcaa agcgcagccc agaacctcag
    12181 ccaacaatcg ggcaacgtac ggggaaagat tggccgatcc atgatgtcag cagccctaac
    12241 ccaaagcgga ctagcgcata ccgcccctct gactccgcca tcccagggct cgagaagctt
    12301 ccgtggcgtc gatataaatt cagcgggcct tgaacatccc tccttacgac acacctcacg
    12361 cgatcgattt tgacactcac acaccgccac cctcacatcc tccacccaca ccacacccct
    12421 taatcaaccc accatcaccg ctagaacgtc tatctcatca ccgacttctc atccatcttc
    12481 aaacatgcac acgctgatga gtccgtgagg acgaaacgag taagctcgtc cgtgtgaaga
    12541 tatgatgcgg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
    12601 ttgaaaaagt ggcaccgagt cggtgctttt ggccggcatg gtcccagcct cctcgctggc
    12661 gccggctggg caacatgctt cggcatggcg aatgggacag ctttggactg cttgatccac
    12721 ttaacgttac tgaaatcatc aaacagcttg acgaatctgg atataagatc gttggtgtcg
    12781 atgtcagctc cggagttgag acaaatggtg ttcaggatct cgataagata cgttcatttg
    12841 tccaagcagc aaagagtgcc ttctagtgat ttaatagctc catgtcaaca agaataaaac
    12901 gcgtttcggg tttacctctt ccagatacag ctcatctgca atgcattaat gcattggacc
    12961 tcgcaaccct agtacgccct tcaggctccg gcgaagcaga agaatagctt agcagagtct
    13021 attttcattt tcgggagacg agatcaagca gatcaacggt cgtcaagagt cctacgagac
    13081 tgaggaatcc gctcttggct ccacgcgact atatatttgt ctctaattgt actttgacat
    13141 gctcctcttc tttactctga tagcttgact atgaaaattc cgtcaccagc ccctgggttc
    13201 gcaaagataa ttgcactgtt tcttccttga actctcaagc ctacaggaca cacattcatc
    13261 gtaggtataa acctcgaaaa tcattcctac taagatgggt atacaatagt aaccatgcat
    13321 ggttgcctag tgaatgctcc gtaacaccca atacgccggc cgaaactttt ttacaactct
    13381 cctatgagtc gtttacccag aatgcacagg tacacttgtt tagaggtaat ccttctttct
    13441 agaagtcctc gtgtactgtg taagcgccca ctccacatct ccactcgctc cggggagaac
    13501 cgaagaggat gggaaccccg accatggcct gcggccgcag ctatcagtcc tctcagctga
    13561 cgcccctcac gaaccctcgc actctcccga aatgctcttc ggtgtccatg gcgcatcatc
    13621 tcagcagcat cccatgtcaa accatggctt tgggcccacg gactctgtgg ccctgccgca
    13681 acatcaccat caccatcgac tcccgcccca tgcagcgctg cgcgccccag ggcgtcatgg
    13741 agtgaatgtg gaatctcctc ctttgccctc gggcccgccg agtgtggtgg gccagcctgg
    13801 aatgcctgat ccagccccca ggcctcgagg accaaaactg aagtttactc ccgaagagga
    13861 cgctctactg gtggagttga aggaaaacaa gaacttgacg tggaagcaaa ttgcagactt
    13921 cttcccgggc cgaacgagcg gtaccttgca agtccgatac tgcaccaagc tgaaggctaa
    13981 ggatgtagct tggagtgacg aaatggttcg atttgctcct gatgtatttc tacgcctgtc
    14041 tcacacatgc taatgaaggg aataggtaca aaggctgcag cgggcaatgc acgagtacga
    14101 gaacgatcgg tggcgcatca ttgcagggaa ggttggaaat ggcttcaccc cagctgcttg
    14161 ccgcgagaaa gccatgcagc tccatgagta aaagcgttgg ggaattttca tatttatatc
    14221 tactgtcgcc agattcggcc ctgcttggac cctctgatct ccttactctc catattggtt
    14281 caaatgtcgg gtcaccgata gggctggtgg tgcaggcttg ttgtaggcac gggaggatga
    14341 tcagcataac tctgagtcac tatagggacg ggttgatgta aggtattaag tgatgtatga
    14401 taattcattt tagcccgggg gaacatatgg cgccggcatt tgttcgttcg caatgaaccg
    14461 acactagcgt ccgctctcgc agtttagcac cggctgatcc cgggctgaac gcggccattg
    14521 ctcggccggg gcatgtgttc cttatctacg gcagaccgca gatgaccact ggagcagatt
    14581 atagacccta agccctaagc cggacaccca atcgagtagg tctgcggacc aggtcactgc
    14641 gggcagccgg agaagctccg caaccaatca atccccggcg ctgactaagg gcaggcgacc
    14701 acgggccgaa gcggcttcaa actcacctca acctccaaac tccctcatct ccaaacgtcc
//
