LOCUS       sequence-237904-        7765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RpoD6 epitope tagged construct  for integration at neutral site 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Fleming KE, O'Shea EK
  TITLE     An RpaA-Dependent Sigma Factor Cascade Sets the Timing of Circadian 
            Transcriptional Rhythms in Synechococcus elongatus.
  JOURNAL   Cell Rep. 2018 Dec 11;25(11):2937-2945.e3. doi: 
            10.1016/j.celrep.2018.11.049.
  PUBMED    30540929
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-11- 11"; volume: "25"; issue: "11"; pages: "2937-2945.e3"
COMMENT     Sequence Label: KFP14
FEATURES             Location/Qualifiers
     source          1..7765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1704..1769
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3049..3840
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5994..6582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6483..6502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6736..6753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6768..6908
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6834..6853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6994..7016
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgcagcgggc gagtcggatt gacccggtag ggatttcgcc agatcaatgc ccgtggtttg
       61 tttcagcttc tccagcaagc tagcgatttg ggtagcgctg ccttcccctt cgccaatcac
      121 agtgatcgac tccacgtcga tatctggcac ggtgcctgaa agcgtgacga gcagggactc
      181 gaagcttagt ggagttcagc atcgccccac tttagcaaga caaaagggca agaccctagg
      241 ccccgcccaa cagcgactga gaccagcggt cgcaatcgaa tgagtaacga gattgtaaaa
      301 aactgatgat gcgatcgttg atttcgtgat gttgagaggt cttacttttt aatcactaaa
      361 atcaacaata tttatcaagg caactatttc gagacactga tttgggtttg accgcgagtg
      421 ctccctagcc gatcgataac ccgccactgc tgctctttcg gctggtagag cttttgcagg
      481 aactcaagcg ccgactggcg gtcgcaaaac tctaagaatt gaccattcac ttctacgaag
      541 aaaggcgcgg gtaacttgta gtcagccata cagaaatttt tcagaactca gcagaatgcc
      601 ccaaggatgg cagaacttcg ttaagctgta gttatttaaa aatcatggag aagtgaagaa
      661 actgtaatcc ctcttacatt ttcgacacat tcctgatctg aagataacct taagagcctt
      721 ggttaaaatc aacttcttgc tgcatttttc tcttaatgct ttcgggggtg cgtatggcga
      781 gtgcttcggc tcatgctgtc gcgacggctc agcgacgaac aagagataac gtcagttggt
      841 atctcagctc catcggtcgg attccattgc tcaccgccga tcaggagatc gagctggggc
      901 agaaagtgca ggtgatgatg aacctgctgg aagaaacttc tagcgatcgc acgtttactg
      961 aggccgaaac cgcagcgatc cagctggggc agcgtgctaa ggaacggatg atcaaggcca
     1021 acttgcgtct tgtggtctct gtggccaaaa agtaccaagg taagggtctt gagtttcttg
     1081 acctgatcca agaaggcgct ctcgggctag aacgtgctgt cgagaaattt gacccctcca
     1141 agggctacaa gttttccacc tatgcctttt ggtggattcg ccagagcatg acaagggcga
     1201 tcgcctgcca atcgcgcact attcgcctgc cggttcactt gactgaacgg ctgagccaaa
     1261 tccgcaaagt gactcgggaa ctggcgcacg agctaggccg tttgccctct cgccaagaaa
     1321 ttgcagaagc ggttcagttg ccactgaatg acctcgaaac aattctgcgt cagtcgcgcc
     1381 aatgcaccag cctagatgct ccagtcagtg gtgaagatgg caagagtttc ctgggtgagc
     1441 tgattgctga tcccaacggc aatgagccca tggacatcat ggaaaatgcc atgcagcgcg
     1501 agcagcttga tgcttggctc gagcatctca cgccccaaga gcgccaggtg ctctatctcc
     1561 gctttggact gggtgatggc gaacgtcata ccctcgctga aatcggccgg atgatggaag
     1621 tctcgcgcga gcgggtccgc caagttgagg tcaaagcgct gcgtaagctc cgcaacctga
     1681 cgaagcgatc gcggattgct gtcgactaca aggaccacga cggtgactac aaggaccacg
     1741 acatcgacta caaggacgac gacgacaagt gagtcgcttc cctgtccagt cctcctgtct
     1801 gtcttaagca gcttaggcgc gatcgcctag gctgtttttt tgaccgtcca ttgcgtgatc
     1861 ggtgccagcg atttccagcc gagaaagcgg ccgatcggtt ccgctcgctg caccgccaca
     1921 cggattaaat ctccccccca gcgctgctgt ccagccaaaa cgacttgctc gctttcgagg
     1981 gtaacggcgt taacaactag ccgcccaccc atcggcagcg cttgccagca ttgatcgagc
     2041 acacctggag cagtgacacc gccaccgatg aagatggcat ccggttgggg caagccactc
     2101 agcgtagccg gtgctgaacc ggcaaccact tgcaggttcg gaaccccgag ggcgatcgca
     2161 ttctgtgtaa gcttgcatgc ctgcaggtcg actctagagc tttatgcttg taaaccgttt
     2221 tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta attagtaata
     2281 taatctatta aaggtcattc aaaaggtcat ccaccggatc aattcccctg ctcgcgcagg
     2341 ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc
     2401 acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg
     2461 atccgcatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa
     2521 ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga
     2581 ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac
     2641 agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc
     2701 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg
     2761 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     2821 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2881 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     2941 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     3001 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     3061 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     3121 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     3181 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     3241 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     3301 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     3361 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     3421 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     3481 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     3541 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     3601 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     3661 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     3721 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     3781 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     3841 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     3901 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     3961 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgaaag gctggctttt
     4021 tcttgttatc gcaatagttg gcgaagtaat cgcaacatcc gcattaaaat ctagcgaggg
     4081 ctttactaag ctgatccggt ggatgacctt ttgaatgacc tttaatagat tatattacta
     4141 attaattggg gaccctagag gtcccctttt ttattttaaa aattttttca caaaacggtt
     4201 tacaagcata ctagaggatc ggcggccgcg gatccccggg taccgagctc gaatttcgag
     4261 cttctggagc aggaagatgt cgcgggcatt agcaccagcg gtctgccaag cctccgccag
     4321 ccgttgggtc ccttccgctt gagcttttcc atcttcgacg atacgggcgg cggccccccg
     4381 cgcttccgcg atcgcccgtt tacaagctgc ctcagctggg gcgatcacat cggcttgaag
     4441 ttgctgctgc acctgtttga tccgctcctg ctgcacaggg agttctgctt ggctacgagc
     4501 gacttcggta gcaatgtccg cttcagcttc ggccaccacc gcttcgcgcc gcgtcaacgc
     4561 atcctgaatc cggcgctcgg cctcggcttg ggcgatcgct acatcgcgat cgatccgacg
     4621 cagggccgtg atcttgtcat tttcggccgt ttggatcgca gaggcagcct gggcatcggc
     4681 ttcagcaatt cgggcatctc gctgcagatc agcccgctgc ttgcgtccac tagccgagag
     4741 ataaccgacc tcatcggaaa tgttctggac ttgcagcgta tcgaggacta gacccagctg
     4801 ctcaaggtca tcctccgcct cttccagcag acttttggca aaggcaattt tgtcctcgtt
     4861 gatctgctcc ggcgtgaggc tggctaaaac accacgcaag ttgccttcga gggtctcctt
     4921 ggcaatttgc tcgatttcct tacggttttt gccaagcagc cgctcgatcg cgttgtggat
     4981 ggtcggttct tccccagcaa tcttgatatt ggcaacgcct tcaacagtca ggggaatgcc
     5041 gcccttggag aaggcattgg aaacgcgcaa ctcaatgatc atgttggtca gatccatgcg
     5101 gagcgctttt tccagcagag gtacccgcag gctgctgccg cccttgacca agcgatagcc
     5161 aactcggcgg ccatcactac tgcggcgact actgccagca aagatcaaaa tttcactggg
     5221 ttggcagatg tagtagagat tgcgcaggac taagctgcca gccccggcga ctcaccagtc
     5281 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5341 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5401 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5461 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     5521 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5581 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5641 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5701 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5761 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5821 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5881 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6121 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     6781 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     6841 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     6901 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6961 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7021 caccgtcatc accgaaacgc gcgaggcagg atcgatccgg cagccggcgg agcgctgctt
     7081 tcttggcaag cggtcgccag ccccaacgcc agggctgcca gcccgaaaca gcggggcaag
     7141 gcagcttgga agggcgatcg cagcacgggc atggcaatgt ctctctgaag gaatgcagac
     7201 cttattcgta cagccagggt tgaatcgtgg gggtccaatc acttagctct gctgggctaa
     7261 accagagagc aatttcctgt tgtgctgttt cgattgcatc cgagccatgg atgatgttgc
     7321 ggccaatatt gacaccaaaa tcaccacgga tggtgcccgg ttctgccgtc agcggattgg
     7381 tagcgccgat caacttgcga gcagccgcca caacgccttc gccttccaag acgatcgcca
     7441 cgatcggccc agaggtgatg aactcgacga ggccattgaa gaaggggcgc tcgcggtgga
     7501 cagcatagtg ctgttcggcc agctcgcgac tgggcttcag ctgctttagg cccaccagtt
     7561 tgaagccttt ttgctcaaag cggccgatga tcgtaccgac caaaccccgc tgaacgccat
     7621 cgggcttgat ggcaataaat gtgcgttcca cagacatcta gatagtcctc aagacgaggc
     7681 aagcattgag cttgccttcc tatggttcgg gatcactggg attcttgaca agcgatcgcg
     7741 gtcacatcgc tatctcttag gactt
//
