LOCUS       sequence-237906-        8745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, 
            Ting A
  TITLE     Directed Evolution of Split APEX2 Peroxidase.
  JOURNAL   ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919.
  PUBMED    30848125
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919."
COMMENT     Sequence Label: FKBP-V5-AP-nes_pLX304
FEATURES             Location/Qualifiers
     source          1..8745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             985..1026
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1684..1710
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    1752..2340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1805..1825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2223..2234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2412..2645
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2723..2844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2760..2779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2814..2833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2884..3019
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3039..3058)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3040..3058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3068..3085)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3068..3084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3077..3099)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3226..3681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3313..3332)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3523..3544
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3707..3811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3812..3880,3881..4672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4030..4049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4843..5431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5332..5351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5585..5602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5719..5740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5755..5760,5761..5778,5779..5785)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5793..5809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5798..5820
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5817..5833
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5817..5833
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5852..5872
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5854..5872
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5900..6126
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6127..6307
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6354..6479
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6972..7205
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7390..7434
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7609..8109
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8028..8046
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8121..8516
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8576..8693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgc aggtggaaac catctcccca
      661 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg
      721 cttgaagatg gaaagaaatt tgattcctcc cgggacagaa acaagccctt taagtttatg
      781 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
      841 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
      901 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaagggagt
      961 ggcagcggag gtagtgggag cggtggcaag cccatcccca accccctgct gggcctggac
     1021 agcaccaagg gctcgggctc gacctcgggc tcgggctcga cctcgggctc gggcaccggt
     1081 gggaaatcat acccaacagt gtccgcagac taccaggatg ccgtggagaa agccaagaag
     1141 aggctgggag ggtttatcgc agaaaagagg tgcgcacctc tgatgctgag actggctttc
     1201 cacagcgcag gcacctttga caagagaacc aaaacaggcg gaccctttgg aacaatcagg
     1261 taccccgctg aactggcaca tagtgccaac agcgggctgg acatcgccgt gcggctgctg
     1321 gaacctctga aagcagagtt cccaattctg tcctacgccg atttttatca gctggcagga
     1381 gtggtcgctg tggaggtcac tgggggcccc aaggtgcctt ttcacccagg acgggaggac
     1441 aagccagaac tacctccaga ggggcgcctg ccagatccga caaagggctc cgaccatctg
     1501 cgagatgtgt ttgggaaagc tatgggcctg actgaccagg atatcgtcgc actgtctgga
     1561 gggcacaccc ttggcgccgc tcataaggaa aggtcaggct tcgagggacc ctggacaagc
     1621 aaccccctgg ttttcgacaa ttcttacttt actgaactgc tgagtggaga gaaggaactg
     1681 cagctgcctc ccctggagcg cctgaccctg gactaataag ctagcgctaa ccggtggcgc
     1741 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1801 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1861 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     1921 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     1981 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2041 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2101 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2161 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2221 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2281 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2341 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2401 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2461 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2641 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     2701 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     2761 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2821 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     2881 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2941 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3001 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3061 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3121 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3181 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3241 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3301 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3361 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3421 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3481 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3541 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3601 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3661 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4021 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4321 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4501 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4681 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4981 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5461 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5521 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5581 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5641 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5701 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5761 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5821 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     5881 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     5941 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6001 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6061 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6121 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6181 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6241 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6301 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6361 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6421 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6481 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6541 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6601 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     6661 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     6721 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     6781 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     6841 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     6901 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     6961 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7021 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7081 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7141 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7201 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7261 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7321 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7381 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7441 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7501 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7561 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     7621 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     7681 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     7741 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     7801 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     7861 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     7921 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     7981 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     8041 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     8101 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     8161 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     8221 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8281 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8341 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8401 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8461 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     8521 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     8581 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8641 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8701 attacaggga cagcagagat ccactttggc gccggctcga ggggg
//
