LOCUS       sequence-237912-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, 
            Ting A
  TITLE     Directed Evolution of Split APEX2 Peroxidase.
  JOURNAL   ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919.
  PUBMED    30848125
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919."
COMMENT     Sequence Label: NLS-MCP-AP_pLX304
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    156..273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        344..1555
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1392..1411
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1584..1608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1632..1652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1659..1700
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2768..3356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2821..2841)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3239..3250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3428..3661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3739..3860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3776..3795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3830..3849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3900..4035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4055..4074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4056..4074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4084..4101)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4084..4100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4093..4115)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4242..4697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4329..4348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4539..4560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4723..4827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4828..4896,4897..5688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5046..5065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5859..6447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6348..6367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6601..6618
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6735..6756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6771..6776,6777..6794,6795..6801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6809..6825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6814..6836
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6833..6849
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6833..6849
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6868..6888
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6870..6888
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6916..7142
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7143..7323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7370..7495
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7988..8221
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8406..8450
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8625..9125
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9044..9062
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9137..9436,1..96)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
       61 cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaagaattc
      121 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      181 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      241 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      301 ccactttggc gccagctcga gggggcccgg ggttattaga tctggcctcc gcgccgggtt
      361 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
      421 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
      481 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
      541 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
      601 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
      661 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
      721 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
      781 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
      841 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
      901 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
      961 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     1021 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     1081 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     1141 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     1201 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1261 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1321 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     1381 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     1441 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     1501 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacagagc
     1561 tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggcttc gaaggagata
     1621 gaaccatggc tccaaagaag aagcgtaagg taggcagcgg caagcccatc cccaaccccc
     1681 tgctgggcct ggacagcacc ggcagcgcta gcaatttcac tcaatttgtg ctcgtggata
     1741 acggaggaac gggcgacgtg accgtggcac caagcaactt tgctaatgga gtggccgagt
     1801 ggattagctc aaatagcagg tcccaggctt acaaggtcac ctgtagtgtc cggcaatcat
     1861 ccgcccagaa tcggaaatat actattaagg tagaagtgcc gaaagtggca actcaaaccg
     1921 tggggggcga ggaattgcct gtggccggct ggagaagcta cctcaacatg gaactcacca
     1981 ttccaatctt cgccacaaac agcgactgtg agctcatcgt caaagccatg cagggtctgc
     2041 tgaaggacgg caatcctatt ccatctgcaa tcgcggccaa ttccggcatt tatggatcca
     2101 agggctcggg ctcgacctcg ggctccggag ggaaatcata cccaacagtg tccgcagact
     2161 accaggatgc cgtggagaaa gccaagaaga ggctgggagg gtttatcgca gaaaagaggt
     2221 gcgcacctct gatgctgaga ctggctttcc acagcgcagg cacctttgac aagagaacca
     2281 aaacaggcgg accctttgga acaatcaggt accccgctga actggcacat agtgccaaca
     2341 gcgggctgga catcgccgtg cggctgctgg aacctctgaa agcagagttc ccaattctgt
     2401 cctacgccga tttttatcag ctggcaggag tggtcgctgt ggaggtcact gggggcccca
     2461 aggtgccttt tcacccagga cgggaggaca agccagaact acctccagag gggcgcctgc
     2521 cagatccgac aaagggctcc gaccatctgc gagatgtgtt tgggaaagct atgggcctga
     2581 ctgaccagga tatcgtcgca ctgtctggag ggcacaccct tggcgccgct cataaggaaa
     2641 ggtcaggctt cgagggaccc tggacaagca accccctggt tttcgacaat tcttacttta
     2701 ctgaactgct gagtggagag aaggaataat gataagctag cgctaaccgg tggcgcgtta
     2761 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2821 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2881 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2941 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3001 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3061 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3121 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3181 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3241 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3301 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     3361 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3421 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     3481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3661 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3721 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3781 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3841 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3961 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4021 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4081 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4141 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4201 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4261 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4321 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4381 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4441 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4501 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4561 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4621 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4681 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4801 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4861 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4921 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4981 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5041 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5101 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5161 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5221 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5281 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5341 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5401 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5461 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5521 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5581 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5641 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5701 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5761 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5821 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5881 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5941 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6001 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6061 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6121 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6181 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6241 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6301 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6361 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6421 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6481 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6541 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6601 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6661 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6721 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6781 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6841 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6901 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6961 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     7021 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7081 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7141 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7201 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7261 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7321 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7381 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7441 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7501 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7561 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7621 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7681 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7741 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7801 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7861 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7921 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7981 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8041 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8101 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8161 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8221 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8281 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8341 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8401 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8461 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8521 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8581 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     8641 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     8701 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     8761 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     8821 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     8881 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     8941 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     9001 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     9061 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     9121 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     9181 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     9241 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     9301 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     9361 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     9421 ggtgcttctc gatctg
//
