LOCUS       sequence-237913-        9120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian 
            cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, 
            Ting A
  TITLE     Directed Evolution of Split APEX2 Peroxidase.
  JOURNAL   ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919.
  PUBMED    30848125
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919."
COMMENT     Sequence Label: HA-Halotag-FRB-EX-nes_pLX304
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2059..2085
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    2127..2715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2180..2200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2598..2609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2787..3020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3098..3219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3135..3154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3189..3208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3259..3394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3414..3433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3415..3433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3443..3460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3443..3459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3452..3474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3601..4056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3688..3707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3898..3919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4082..4186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4187..4255,4256..5047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4405..4424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5218..5806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5707..5726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5960..5977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6094..6115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6130..6135,6136..6153,6154..6160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6168..6184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6173..6195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6192..6208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6192..6208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6227..6247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6229..6247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6275..6501
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6502..6682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6729..6854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7347..7580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7765..7809
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7984..8484
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8403..8421
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8496..8891
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8951..9068
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt acccatacga cgttccagac
      661 tacgcaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
      721 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
      781 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
      841 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
      901 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
      961 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
     1021 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
     1081 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
     1141 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
     1201 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
     1261 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
     1321 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
     1381 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
     1441 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
     1501 atcggcagtg agatcgcgcg ctggctctcg acgctcgaaa tttccggcgc tagcaccaaa
     1561 ggttctggtg ggactggcgg gagcgtggcc atcctgtggc acgagatgtg gcatgaaggc
     1621 ctggaagagg catctcgttt gtactttggg gaaaggaacg tgaaaggcat gtttgaggtg
     1681 ctggagccct tgcatgctat gatggaacgg ggcccccaga ctctgaagga aacatccttt
     1741 aatcaggcct atggtcgaga tttaatggag gcccaagagt ggtgcaggaa gtacatgaaa
     1801 tcagggaatg tcaaggacct cacccaagcc tgggacctct attatcatgt gttccgacga
     1861 atctcaggat ccaagggctc gggctcgacc tcgggctcgg gcgggctgct gcagctgccc
     1921 agcgacaaag ccctgctgtc cgatcccgtg ttcagacctc tggtcgataa gtatgcagcc
     1981 gacgaggatg cttttttcgc agattacgca gaagcacatc agaagctgtc agaactggga
     2041 tttgccgacg ccctgcagct gcctcccctg gagcgcctga ccctggacta ataagctagc
     2101 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     2161 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2221 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2281 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2341 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2401 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2461 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2521 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2581 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2641 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg ttctcccttt
     2701 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     2761 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2821 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     2881 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2941 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3001 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     3061 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     3121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     3241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3361 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     3421 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     3481 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     3541 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     3601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4021 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5041 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5881 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5941 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6001 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6061 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     6241 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     6301 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     6361 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     6421 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     6481 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     6541 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6601 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6661 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     6721 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     6781 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     6841 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     6901 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     6961 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7021 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7081 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7141 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7201 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7261 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7321 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7381 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     7441 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     7501 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     7561 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     7621 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     7681 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     7741 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     7801 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     7861 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     7921 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     7981 cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     8041 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     8101 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     8161 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     8221 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     8281 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     8341 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     8401 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     8461 cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg tctcaagaag
     8521 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     8581 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     8641 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     8701 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     8761 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     8821 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     8881 tgtgggaggg cctgcagctg cagtagtaag aattctagat cttgagacaa atggcagtat
     8941 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9001 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9061 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
//
