LOCUS       sequence-237915-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian 
            mitochondrial outer membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, 
            Ting A
  TITLE     Directed Evolution of Split APEX2 Peroxidase.
  JOURNAL   ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919.
  PUBMED    30848125
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919."
COMMENT     Sequence Label: Tom20-V5-FKBP-AP_pLX304
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    156..273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        344..1555
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1392..1411
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1584..1608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1758..2078
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2109..2150
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2840..3428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2893..2913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3311..3322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3500..3733
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3811..3932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3848..3867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3902..3921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3972..4107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4127..4146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4128..4146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4156..4173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4156..4172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4165..4187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4314..4769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4401..4420)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4611..4632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4795..4899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4900..4968,4969..5760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5118..5137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5931..6519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6420..6439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6673..6690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6843..6848,6849..6866,6867..6873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6886..6908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6905..6921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6905..6921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6940..6960
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6942..6960
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6988..7214
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7215..7395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7442..7567
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8060..8293
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8478..8522
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8697..9197
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9116..9134
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9209..9508,1..96)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
       61 cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaagaattc
      121 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      181 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      241 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      301 ccactttggc gccagctcga gggggcccgg ggttattaga tctggcctcc gcgccgggtt
      361 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
      421 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
      481 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
      541 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
      601 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
      661 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
      721 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
      781 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
      841 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
      901 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
      961 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     1021 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     1081 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     1141 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     1201 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1261 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1321 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     1381 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     1441 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     1501 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacagagc
     1561 tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggcttc gaaggagata
     1621 gaaccatggt gggtcggaac agcgccatcg ccgcgggcgt gtgcggtgcc ctcttcatag
     1681 ggtactgcat ctactttgac cgcaaaagac gaagtgaccc caacttcgga tccggagcaa
     1741 gtggaagtgc tggtggtgga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct
     1801 tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga
     1861 aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg
     1921 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga
     1981 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg
     2041 ccactctcgt cttcgatgtg gagcttctaa aactggaagg gagtggcagc ggaggtagtg
     2101 ggagcggtgg caagcccatc cccaaccccc tgctgggcct ggacagcacc aagggctcgg
     2161 gctcgacctc gggctcgggc tcgacctcgg gctcgggcac cggtgggaaa tcatacccaa
     2221 cagtgtccgc agactaccag gatgccgtgg agaaagccaa gaagaggctg ggagggttta
     2281 tcgcagaaaa gaggtgcgca cctctgatgc tgagactggc tttccacagc gcaggcacct
     2341 ttgacaagag aaccaaaaca ggcggaccct ttggaacaat caggtacccc gctgaactgg
     2401 cacatagtgc caacagcggg ctggacatcg ccgtgcggct gctggaacct ctgaaagcag
     2461 agttcccaat tctgtcctac gccgattttt atcagctggc aggagtggtc gctgtggagg
     2521 tcactggggg ccccaaggtg ccttttcacc caggacggga ggacaagcca gaactacctc
     2581 cagaggggcg cctgccagat ccgacaaagg gctccgacca tctgcgagat gtgtttggga
     2641 aagctatggg cctgactgac caggatatcg tcgcactgtc tggagggcac acccttggcg
     2701 ccgctcataa ggaaaggtca ggcttcgagg gaccctggac aagcaacccc ctggttttcg
     2761 acaattctta ctttactgaa ctgctgagtg gagagaagga ataataagct agcgctaacc
     2821 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2881 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2941 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3001 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3061 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3121 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3181 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3241 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3301 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3361 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3421 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3481 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3541 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3601 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3661 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3721 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3781 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3841 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3901 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3961 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4021 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4081 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4141 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4201 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4261 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4321 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4381 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4441 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4501 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4561 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4621 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4681 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4741 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6901 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6961 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7021 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7081 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7141 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7201 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7381 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7441 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7501 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7561 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7621 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7681 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7741 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7801 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7861 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7921 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7981 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8041 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8101 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8161 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8221 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8281 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8341 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8401 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8461 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8521 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8581 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8641 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     8701 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     8761 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     8821 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     8881 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     8941 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     9001 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     9061 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     9121 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     9181 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     9241 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     9301 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     9361 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     9421 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     9481 acggtgccga caggtgcttc tcgatctg
//
