LOCUS       sequence-237920-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00131951
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(469..485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(469..485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(482..504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    493..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(517..523,524..541,542..547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    562..583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(700..717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acttggtctc taagctcatt ttctatcaca ttcaaaaata gaaacttgaa aatcagctgg
       61 accagtatat ctttgagttt gaacagcacc attattatca gtaaccaatt gatcagctgg
      121 aattttatga caataatctt gattagttct agaattaatt ggttgagaag cagccaaata
      181 caaaccacca tcttgtggtt taacatcaaa tttaaatgga gaattttcac atggagcttg
      241 agtaacagct ggcaatttat ctggaccatt caaagtaatt tcacaatgag aaccttctgg
      301 aaaaacagct ggatcagaag taatattaaa agaataagta catctaacag aatgtggaat
      361 acaagcagct tgaaaagatt taacaaaaaa agcttgttgt cttggaacca aagtagccat
      421 tagagaccaa gttcatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt
      481 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      541 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      601 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      661 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      781 cacagaatca
//
