LOCUS       sequence-237925-        1345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: N236Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pcDNA-PU.1-ETS N236Q
FEATURES             Location/Qualifiers
     source          1..1345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(547..564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    553..777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(911..930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gnnntagcag agctctctgg ctnctagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagctgg ctagcgctac cggtcgccac catgggatcg
      121 aaaaaaaaga tccgccttta ccaattctta ctggaccttc tgcgctctgg agatatgaaa
      181 gactcaattt ggtgggttga caaagacaaa ggcacttttc aattcagttc aaagcataag
      241 gaagctctgg cccatcgttg gggaattcaa aaaggtaacc gcaagaaaat gacttaccag
      301 aaaatggcgc gtgccttgcg tcaatacgga aaaacagggg aggtgaagaa agtaaagaaa
      361 aaacttacat atcaattcag cggggaagtt ttaggacgcg gtggcttggc tgaacgccgt
      421 cacccacctc attgaaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
      481 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
      541 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      601 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      661 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      721 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
      781 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
      841 cattaagcgc ggcgggngnt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      961 cgtcaagctc taaatcgggg gctccccttt agggttccga tttagtgctt tacggcacct
     1021 cgaccccaaa aaacttgatt aagggtgatg gnttcacgta atggggccat cgccntgaaa
     1081 aancggtttt tcgcccnttt gacgttggna gtccacgttc ctttaataag tggaactctt
     1141 ggttccaaac tggaancaac actcaacccc tatctccggn nnnttctttn gaattnnnaa
     1201 gggaattttg ccnaatttcc ggcctattgg gttaaaaaat gaanctgaat ttaacaaaaa
     1261 ntttancnnn naattaatnn nggnnaanng nnnnnnnntt naggnnnngg naangtcccc
     1321 naggcttccc naaccnggnn aaant
//
