LOCUS       sequence-237926-        2287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the component plasmid of a regulated device.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Huang HH, Qian Y, Del Vecchio D
  TITLE     A quasi-integral controller for adaptation of genetic modules to 
            variable ribosome demand.
  JOURNAL   Nat Commun. 2018 Dec 21;9(1):5415. doi: 10.1038/s41467-018-07899-z.
  PUBMED    30575748
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-21"; volume: "9(1):5415. doi"; pages: " 
            10.1038/s41467-018-07899-z"
COMMENT     Sequence Label: pHH04_32_sRNA-A_Sp_coD
FEATURES             Location/Qualifiers
     source          1..2287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(944..962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1360..2151
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      2182..2276
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ccaattattg aacacccttc ggggtgtttt tttgtttctg gtctcccgcg actttattta
       61 acagcggcat ggccagggaa ccgatgcgtc aatcgcacca cacaatgttt gacctgatga
      121 gtccgtgagg acgaaacgag ctagctcgtc gtcaaagttt gttatatttg tagaaatatt
      181 ttattcgccc ccggaagatc attccggggg cttttttatt cgttcggctg cggcgagcgg
      241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gccctcgagg
      361 agcgcctcag cgcgccgtag cgtcgataaa aattacgggc tggggcgaaa ctaccatctg
      421 ttcgaaaagg tccgtaaatg ggcctacaga gcgattcgtc agggctggcc tgtattctca
      481 caatggcttg atgccgttat ccagcgtgtc gaaatgtaca acgcttcgct tcccgttccg
      541 ctttctccgg ctgaatgtcg ggctattggc aagagcattg cgaaatatac acacaggaaa
      601 ttctcaccag agggattttc cgctgtacag gccgctcgcg gtcgcaaggg cggaactaaa
      661 tctaagcgcg cagcagttcc tacatcagca cgttcgctga aaccgtggga ggcattaggc
      721 atcagtcgag cgacgtacta ccgaaaatta aaatgtgacc cagacctcgc aaaatgagac
      781 cagataagcc ttatcagata acagcgccct tttggcgtct ttttgagcac ctaggtgtaa
      841 aacacggcag tcttaagctc gggccctctt ttctacgggg tctgacgctc agtggaacga
      901 aaactcacgt taagggattt tggtcatgag tgaagacgaa agggcctcgt gatacgccta
      961 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcggaatt gccagctggg
     1021 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     1081 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
     1141 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1201 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     1261 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1321 acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc
     1381 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1441 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     1501 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1561 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     1621 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1681 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     1741 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1801 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1861 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1921 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1981 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     2041 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     2101 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata agcgggactc
     2161 tggggttcga gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     2221 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     2281 gcactag
//
