LOCUS       sequence-237930-        1355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: N236Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pcDNA-PU.1-ETS N236Y
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(550..567)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    556..667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(919..938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gnnnnngcag ngnttnnctg gctaactana gaacccacng gcttacnggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgc taccggtcgc caccatggga
      121 tcgaaaaaaa agatccgcct ttaccaattc ttactggacc ttctgcgctc tggagatatg
      181 aaagactcaa tttggtgggt tgacaaagac aaaggcactt ttcaattcag ttcaaagcat
      241 aaggaagctc tggcccatcg ttggggaatt caaaaaggta accgcaagaa aatgacttac
      301 cagaaaatgg cgcgtgcctt gcgttactac ggaaaaacag gggaggtgaa gaaagtaaag
      361 aaaaaactta catatcaatt cagcggggaa gttttaggac gcggtggctt ggctgaacgc
      421 cgtcacccac ctcattgaaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
      481 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc
      541 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      601 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      661 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggggggggg gggggncagg
      721 acagcaaggg gggaggattg ggnaagacaa tagcaggcat gctggggatg cggtgggctc
      781 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
      841 tagcggcgca ttaagcgcgg cgggtngtgg tggttacgcg cagcgtgacc gctacacttg
      901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      961 ggctttcccc gtcaagctct aaatcggggg gctccctttt agggttccna atttagtgct
     1021 tttacggcca ctcngacccc aaaaancttg attaagggtg atggttncac gtaatggggc
     1081 catcncccct gannanncgg ttttttcccc cntttgaacg ttggnngtcc acgtttcttn
     1141 aannagtgga attcttgttn ccaaactggn aannaanntn caaccntnnt tnncggttct
     1201 attncttttn natttnnaan ggganttttg gccnnatttc cngccntatt ggtttaaaaa
     1261 aatgnncctn natttannna aaaattttaa cccnnaantt naatttccgt ggnaaatggn
     1321 tggtnngntt naaggntnng gaaannttcc ccgnc
//
