LOCUS       sequence-237934-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00141181
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             191..202
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(672..688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(685..707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    696..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(720..726,727..744,745..750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    765..786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(903..920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgactgaac caggtcaatc tccaccaggt agaatgagag aaggttctgc
       61 ttggtttcca ggtgatccaa cttatgattg tggtatttct atttttagac caccaaaacc
      121 aggttctaga tctagaaatg gtgctttggc tgaagttatt tatgttaata gaggtgaaac
      181 ttttaaaatt attgaaggta gatatacttg ttctgttgaa caaaattgtg aaagaccaga
      241 ttgtaatttg ccaagaactt ggaattatag aggtcaatgg ggtttggcta gaccagctag
      301 aaaaaatact tattttgatg aaaatggtgt tcaaaaattt aatactttgg cttctgaatc
      361 tcataaaaga ttgtatgatt gtagagttgc tatttggcaa ccaccaatgg atgctagaac
      421 tactgttgaa actttggctg aagctactca ttttaatttg ggtgaatctg ttagagtttt
      481 gagaggtaga tttacttgta aagctgaaca aaaatgtggt aatgttattt gtaatttgcc
      541 agaaggttat aaatatcaat ctattactgg tggtccattt caaccaatgg aaaaaactag
      601 aaaattttat ccagcttgag cttagagacc aagttccatc ccaatggcgc gccgagcttg
      661 gctcgagcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcga
//
