LOCUS       sequence-237959-       12595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial genome editing plasmid with spectinomycin resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437."
COMMENT     Sequence Label: pCpf1-sp
FEATURES             Location/Qualifiers
     source          1..12595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3923..3957
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(4025..4321)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4171..4193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4185..4202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4186..4202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4212..4231
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4212..4230
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4278..4298)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4278..4296)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4317..4333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4317..4333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4330..4352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4341..4357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4365..4371,4372..4389,4390..4395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4410..4431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             5141..5932
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6711..7105
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7447..7534
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8773..9744
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             10255..11094
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             11081..11932
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     12442..12461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(12565..12570,12571..12588,12589..12594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggaggtcttt atcatgtcaa tttatcaaga atttgttaat aaatatagtt taagtaaaac
       61 tctaagattt gagttaatcc cacagggtaa aacacttgaa aacataaaag caagaggttt
      121 gattttagat gatgagaaaa gagctaaaga ctacaaaaag gctaaacaaa taattgataa
      181 atatcatcag ttttttatag aggagatatt aagttcggtt tgtattagcg aagatttatt
      241 acaaaactat tctgatgttt attttaaact taaaaagagt gatgatgata atctacaaaa
      301 agattttaaa agtgcaaaag atacgataaa gaaacaaata tctgaatata taaaggactc
      361 agagaaattt aagaatttgt ttaatcaaaa ccttatcgat gctaaaaaag ggcaagagtc
      421 agatttaatt ctatggctaa agcaatctaa ggataatggt atagaactat ttaaagccaa
      481 tagtgatatc acagatatag atgaggcgtt agaaataatc aaatctttta aaggttggac
      541 aacttatttt aagggttttc atgaaaatag aaaaaatgtt tatagtagca atgatattcc
      601 tacatctatt atttatagga tagtagatga taatttgcct aaatttctag aaaataaagc
      661 taagtatgag agtttaaaag acaaagctcc agaagctata aactatgaac aaattaaaaa
      721 agatttggca gaagagctaa cctttgatat tgactacaaa acatctgaag ttaatcaaag
      781 agttttttca cttgatgaag tttttgagat agcaaacttt aataattatc taaatcaaag
      841 tggtattact aaatttaata ctattattgg tggtaaattt gtaaatggtg aaaatacaaa
      901 gagaaaaggt ataaatgaat atataaatct atactcacag caaataaatg ataaaacact
      961 caaaaaatat aaaatgagtg ttttatttaa gcaaatttta agtgatacag aatctaaatc
     1021 ttttgtaatt gataagttag aagatgatag tgatgtagtt acaacgatgc aaagttttta
     1081 tgagcaaata gcagctttta aaacagtaga agaaaaatct attaaagaaa cactatcttt
     1141 attatttgat gatttaaaag ctcaaaaact tgatttgagt aaaatttatt ttaaaaatga
     1201 taaatctctt actgatctat cacaacaagt ttttgatgat tatagtgtta ttggtacagc
     1261 ggtactagaa tatataactc aacaaatagc acctaaaaat cttgataacc ctagtaagaa
     1321 agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa tacttatctc tagaaactat
     1381 aaagcttgcc ttagaagaat ttaataagca tagagatata gataaacagt gtaggtttga
     1441 agaaatactt gcaaactttg cggctattcc gatgatattt gatgaaatag ctcaaaacaa
     1501 agacaatttg gcacagatat ctatcaaata tcaaaatcaa ggtaaaaaag acctacttca
     1561 agctagtgcg gaagatgatg ttaaagctat caaggatctt ttagatcaaa ctaataatct
     1621 cttacataaa ctaaaaatat ttcatattag tcagtcagaa gataaggcaa atattttaga
     1681 caaggatgag catttttatc tagtatttga ggagtgctac tttgagctag cgaatatagt
     1741 gcctctttat aacaaaatta gaaactatat aactcaaaag ccatatagtg atgagaaatt
     1801 taagctcaat tttgagaact cgactttggc taatggttgg gataaaaata aagagcctga
     1861 caatacggca attttattta tcaaagatga taaatattat ctgggtgtga tgaataagaa
     1921 aaataacaaa atatttgatg ataaagctat caaagaaaat aaaggcgagg gttataaaaa
     1981 aattgtttat aaacttttac ctggcgcaaa taaaatgtta cctaaggttt tcttttctgc
     2041 taaatctata aaattttata atcctagtga agatatactt agaataagaa atcattccac
     2101 acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa tttgagttta atattgaaga
     2161 ttgccgaaaa tttatagatt tttataaaca gtctataagt aagcatccgg agtggaaaga
     2221 ttttggattt agattttctg atactcaaag atataattct atagatgaat tttatagaga
     2281 agttgaaaat caaggctaca aactaacttt tgaaaatata tcagagagct atattgatag
     2341 cgtagttaat cagggtaaat tgtacctatt ccaaatctat aataaagatt tttcagctta
     2401 tagcaaaggg cgaccaaatc tacatacttt atattggaaa gcgctgtttg atgagagaaa
     2461 tcttcaagat gtggtttata agctaaatgg tgaggcagag cttttttatc gtaaacaatc
     2521 aatacctaaa aaaatcactc acccagctaa agaggcaata gctaataaaa acaaagataa
     2581 tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa gataaacgct ttactgaaga
     2641 taagtttttc tttcactgtc ctattacaat caattttaaa tctagtggag ctaataagtt
     2701 taatgatgaa atcaatttat tgctaaaaga aaaagcaaat gatgttcata tattaagtat
     2761 agatagaggt gaaagacatt tagcttacta tactttggta gatggtaaag gcaatatcat
     2821 caaacaagat actttcaaca tcattggtaa tgatagaatg aaaacaaact accatgataa
     2881 gcttgctgca atagagaaag atagggattc agctaggaaa gactggaaaa agataaataa
     2941 catcaaagag atgaaagagg gctatctatc tcaggtagtt catgaaatag ctaagctagt
     3001 tatagagtat aatgctattg tggtttttga ggatttaaat tttggattta aaagagggcg
     3061 tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa atgctaattg agaaactaaa
     3121 ctatctagtt ttcaaagata atgagtttga taaaactggg ggagtgctta gagcttatca
     3181 gctaacagca ccttttgaga cttttaaaaa gatgggtaaa caaacaggta ttatctacta
     3241 tgtaccagct ggttttactt caaaaatttg tcctgtaact ggttttgtaa atcagttata
     3301 tcctaagtat gaaagtgtca gcaaatctca agagttcttt agtaagtttg acaagatttg
     3361 ttataacctt gataagggct attttgagtt tagttttgat tataaaaact ttggtgacaa
     3421 ggctgccaaa ggcaagtgga ctatagctag ctttgggagt agattgatta actttagaaa
     3481 ttcagataaa aatcataatt gggatactcg agaagtttat ccaactaaag agttggagaa
     3541 attgctaaaa gattattcta tcgaatatgg gcatggcgaa tgtatcaaag cagctatttg
     3601 cggtgagagc gacaaaaagt tttttgctaa gctaactagt gtcctaaata ctatcttaca
     3661 aatgcgtaac tcaaaaacag gtactgagtt agattatcta atttcaccag tagcagatgt
     3721 aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat atgcctcaag atgctgatgc
     3781 caatggtgct tatcatattg ggctaaaagg tctgatgcta ctaggtagga tcaaaaataa
     3841 tcaagagggc aaaaaactca atttggttat caaaaatgaa gagtattttg agttcgtgca
     3901 gaataggaat aactaagaat tcttgacagc tagctcagtc ctaggtataa tgctagcgct
     3961 gatttaggca aaaacgggtc taagaacttt aaataatttc tactgttgta gatgagagca
     4021 ggtgtcagcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     4081 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4141 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4201 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg
     4261 tcctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     4321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4441 cgctcactgc ccgctttcca cccacctgca aaagtctaag aactttaaat aatttctact
     4501 gttgtagatg ctactattcc tgtgccttca gataattcag tctaagaact ttaaataatt
     4561 tctactgttg tagatgtcta gagccttttg tattagtagc cggtctaaga actttaaata
     4621 atttctactg ttgtagatta gcgatttatg aaggtcattt ttttgtctag ctttaatgcg
     4681 gtagttggta ccgtcgatgg cagaaattcg atatctagat ctcatggatc cgcaatggca
     4741 acaacgttgc gctcgactct agaagaccga aaccttgcgc tcgttcgcca gccaggacag
     4801 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     4861 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     4921 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     4981 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     5041 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     5101 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     5161 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     5221 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     5281 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     5341 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     5401 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     5461 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     5521 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     5581 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     5641 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     5701 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     5761 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     5821 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     5881 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     5941 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     6001 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     6061 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     6121 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
     6181 agctgatccg gtggatgacc ttttgaatga cctttaatag attatattac taattaattg
     6241 gggaccctag aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca
     6301 taaagcttgc tcaatctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc
     6361 cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca
     6421 tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg
     6481 tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc
     6541 cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg
     6601 tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc
     6661 cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca cgaacccctg
     6721 caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc
     6781 cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat
     6841 gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc
     6901 cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca
     6961 caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccacacc
     7021 aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt ccccctaccc
     7081 gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca ttcagcccgg
     7141 ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta
     7201 cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca ccttggccaa
     7261 aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt gctgctgttc
     7321 ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt cgagcttggc
     7381 cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt gttaatgtgc
     7441 tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact ttgtagggga
     7501 gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt cgggcggtgc
     7561 gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg cagcatggcg
     7621 atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc cagggccaag
     7681 gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt cttgcacgcc
     7741 gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga tgctgccgac
     7801 ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct gccggtcgag
     7861 ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct gaccggtgcc
     7921 gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc gcactgccac
     7981 ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca gtggttcaag
     8041 cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc gctcaagccc
     8101 aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg ggcattagag
     8161 cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg catcgagcgc
     8221 ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg catccgcacc
     8281 gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat cgacttacag
     8341 gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat ccagaggcat
     8401 caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac tggccgacga
     8461 agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg cggagtggct
     8521 gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt tgcgggaggt
     8581 agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg ggtggcactg
     8641 gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc tgctgatcgc
     8701 atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga tctggctgcg
     8761 cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca gctcaaggcc
     8821 atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca gatgatgaac
     8881 cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg gatgaatgcc
     8941 cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct ggtgctggtg
     9001 gacgacctca gcgagtttga cttggatgac atgaaagccg agggccggga gcctgccctg
     9061 gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga cgccgcaggc
     9121 ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc cgacccggcc
     9181 agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa ggacaagcac
     9241 accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc
     9301 accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc ccagcggcag
     9361 caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct tagccgccac
     9421 cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa gcgcttcggt
     9481 gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg gggccgcagt
     9541 gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga gcgcaagccc
     9601 ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct gcccagcgtc
     9661 cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg catggacagg
     9721 ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt atactatgag
     9781 tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg gtcggtctac
     9841 ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac gtcaaacaag
     9901 gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga cggctgcacc
     9961 ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg tgactgatat
    10021 gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg ctgtacgcca
    10081 agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt tctggctgac
    10141 cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg cgcagggcgg
    10201 gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca atcaatggct
    10261 acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc gctggactac
    10321 gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg tggtgccggt
    10381 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
    10441 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
    10501 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
    10561 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
    10621 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
    10681 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
    10741 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
    10801 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
    10861 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
    10921 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
    10981 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
    11041 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
    11101 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg gcctgttccg
    11161 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
    11221 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
    11281 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
    11341 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
    11401 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
    11461 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
    11521 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
    11581 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
    11641 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
    11701 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggcttc
    11761 catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga ccatgcgcaa
    11821 gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct ggacggtaac
    11881 cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct gacccccccc
    11941 actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt tcctgctaat
    12001 tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt gaaaaattgc
    12061 ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt ccagatgtat
    12121 gctcttctgc tcctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12181 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12241 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12301 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12361 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12421 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12481 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12541 gttcgatgta acccactcgt gcactttaca ctttatgctt ccggctcgta tgtta
//
