LOCUS       sequence-237963-       13414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Zhao X, Sternsdorf T, Bolger TA, Evans RM, Yao TP
  TITLE     Regulation of MEF2 by histone deacetylase 4- and SIRT1 
            deacetylase-mediated lysine modifications.
  JOURNAL   Mol Cell Biol. 2005 Oct;25(19):8456-64.
  PUBMED    16166628
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-10"; volume: "25(19)"; pages: "8456-64"
COMMENT     Sequence Label: pcDNA3-beta-FLAG-CBP-HA
FEATURES             Location/Qualifiers
     source          1..13414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2552..2755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2800..2819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2800..2818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2855..3427
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3473..3496
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8363..8372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10838..10864
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(10904..10922)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10905..10922)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10942..10959)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10948..11172
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11218..11646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11305..11324)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11515..11536
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11655..11675)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11660..11989
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11840..11975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11902..11921
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12056..12850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12110..12129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12720..12739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13024..13145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13061..13080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13115..13134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13194..13210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13194..13210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13207..13229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13218..13234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13242..13248,13249..13266,13267..13272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13287..13308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     2761 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     2821 gacccaagct agagtcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     2881 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     2941 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     3001 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     3061 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     3121 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     3181 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     3241 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     3301 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     3361 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     3421 cctacagctc ctgggcaacg tgctggtttg tgctgtcgac cccaagctta tggactacaa
     3481 ggacgacgac gacaaagccg agaacttgct ggacggaccg cccaacccca aacgagccaa
     3541 actcagctcg cccggcttct ccgcgaatga caacacagat tttggatcat tgtttgactt
     3601 ggaaaatgac cttcctgatg agctgatccc caatggagaa ttaagccttt taaacagtgg
     3661 gaaccttgtt ccagatgctg cgtccaaaca taaacaactg tcagagcttc ttagaggagg
     3721 cagcggctct agcatcaacc cagggatagg caatgtgagt gccagcagcc ctgtgcaaca
     3781 gggccttggt ggccaggctc aggggcagcc gaacagtaca aacatggcca gcttaggtgc
     3841 catgggcaag agccctctga accaaggaga ctcatcaaca cccaacctgc ccaaacaggc
     3901 agccagcacc tctgggccca ctccccctgc ctcccaagca ctgaatccac aagcacaaaa
     3961 gcaagtaggg ctggtgacca gtagtcctgc cacatcacag actggacctg ggatctgcat
     4021 gaatgctaac ttcaaccaga cccacccagg ccttctcaat agtaactctg gccatagctt
     4081 aatgaatcag gctcaacaag ggcaagctca agtcatgaat ggatctcttg gggctgctgg
     4141 aagaggaagg ggagctggaa tgccctaccc tgctccagcc atgcaggggg ccacaagcag
     4201 tgtgctggcg gagaccttga cacaggtttc cccacaaatg gctggccatg ctggactaaa
     4261 tacagcacag gcaggaggca tgaccaagat gggaatgact ggtaccacaa gtccatttgg
     4321 acaacccttt agtcaaactg gagggcagca gatgggagcc actggagtta acccccagtt
     4381 agccagcaaa cagagcatgg tcaatagttt acctgctttt cctacagata tcaagaatac
     4441 ttcagtcacc actgtgccaa atatgtccca gttgcaaaca tcagtgggaa ttgtacccac
     4501 acaagcaatt gcaacaggcc ccacagcaga ccctgaaaaa cgcaaactga tacagcagca
     4561 gctggttcta ctgcttcatg cccacaaatg tcagagacga gagcaagcaa atggagaggt
     4621 tcgagcctgt tctctcccac actgtcgaac catgaaaaac gttttgaatc acatgacaca
     4681 ttgtcaggct gggaaagcct gccaagttgc ccattgtgca tcttcacgac aaatcatctc
     4741 tcattggaag aactgcacac gacatgactg tcctgtttgc ctccctttga aaaatgccag
     4801 tgacaagcga aaccaacaaa ccatcctggg atctccagct agtggaattc aaaacacaat
     4861 tggttctgtt ggtgcagggc aacagaatgc cacttcctta agtaacccaa atcccataga
     4921 ccccagttcc atgcagcggg cctatgctgc tctaggactc ccctacatga accagcctca
     4981 gacgcagctg cagcctcagg ttcctggcca gcaaccagca cagcctccag cccaccagca
     5041 gatgaggact ctcaatgccc taggaaacaa ccccatgagt atcccagcag gaggaataac
     5101 aacagatcaa cagccaccaa acttgatttc agaatcagct cttccaactt ccttgggggc
     5161 taccaatcca ctgatgaatg atggttcaaa ctctggtaac attggaagcc tcagcacgat
     5221 acctacagca gcgcctcctt ccagcactgg tgttcgaaaa ggctggcatg aacatgtgac
     5281 tcaggaccta cggagtcatc tagtccataa actcgttcaa gccatcttcc caactccaga
     5341 ccctgcagct ctgaaagatc gccgcatgga gaacctggtt gcctatgcta agaaagtgga
     5401 gggagacatg tatgagtctg ctaatagcag ggatgaatac tatcatttat tagcagagaa
     5461 aatctataaa atacaaaaag aactagaaga aaagcggagg tcacgtttac ataagcaagg
     5521 catcctgggt aaccagccag ctttaccagc ttctggggct cagccccctg tgattccacc
     5581 agcccagtct gtaagacctc caaatgggcc cctgcctttg ccagtgaatc gcatgcaggt
     5641 ttctcaaggg atgaattcat ttaacccaat gtccctggga aacgtccagt tgccacaggc
     5701 acccatggga cctcgtgcag cctcccctat gaaccactct gtgcagatga acagcatggc
     5761 ctcagttccg ggtatggcca tttctccttc acggatgcct cagcctccaa atatgatggg
     5821 cactcatgcc aacaacatta tggcccaggc acctactcag aaccagtttc tgccacagaa
     5881 ccagtttcca tcatccagtg gggcaatgag tgtgaacagt gtgggcatgg ggcaaccagc
     5941 agcccaggca ggtgtttcac agggtcaggt acctggagct gctctcccta accctctgaa
     6001 catgctggca ccccaggcca gccagctgcc ttgcccacca gtgacacagt caccattgca
     6061 cccgactcca cctcctgctt ccacagctgc tggcatgccc tctctccaac atccaacggc
     6121 accaggaatg acccctcctc agccagcagc tcccactcag ccatctactc ctgtgtcatc
     6181 tgggcagact cctaccccaa ctcctggctc agtgcccagc gctgcccaaa cacagagtac
     6241 ccctacagtc caggcagcag cacaggctca ggtgactcca cagcctcaga ccccagtgca
     6301 gccaccatct gtggctactc ctcagtcatc acagcagcaa ccaacgcctg tgcatactca
     6361 gcctcctggc acaccgcttt ctcaggcagc agccagcatt gataatagag tccctactcc
     6421 ctcctctgtg accagtgctg aaaccagttc ccagcagcca ggacccgatg tgcccatgct
     6481 ggaaatgaag acagaggtgc agacagatga tgctgagcct gaacctactg aatccaaggg
     6541 ggaacctcgg tctgagatga tggaagagga tttacaaggt tcttcccaag taaaagaaga
     6601 gacagatacg acagagcaga agtcagagcc aatggaagta gaagaaaaga aacctgaagt
     6661 aaaagtggaa gctaaagagg aagaagagaa cagttcgaac gacacagcct cacaatcaac
     6721 atctccttcc cagccacgca aaaaaatctt taaacccgag gagctacgcc aggcacttat
     6781 gccaactcta gaagcactct atcgacagga cccagagtct ttgccttttc gtcagcctgt
     6841 agatcctcag ctcctaggaa tcccagatta ttttgatata gtgaagaatc ctatggacct
     6901 ttctaccatc aaacgaaagc tggacacagg gcaatatcaa gaaccctggc agtatgtgga
     6961 tgatgtctgg cttatgttca acaatgcgtg gctatataat cgtaaaacgt cccgtgtata
     7021 taaattttgc agtaaacttg cagaggtctt tgaacaagaa attgaccctg tcatgcagtc
     7081 tcttggatat tgctgtggac gaaagtatga gttctcccca cagactttgt gctgttacgg
     7141 aaagcagctg tgtacaattc ctcgtgatgc agcctactac agctatcaga ataggtatca
     7201 tttctgtgag aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc
     7261 ttcccaacct cagacgacaa tttccaagga tcaatttgaa aagaagaaaa atgatacctt
     7321 agatcctgaa ccttttgttg actgcaaaga gtgtggccgg aagatgcatc agatttgtgt
     7381 tctacactat gacatcattt ggccttcagg ttttgtgtgt gacaactgtt tgaagaaaac
     7441 tggcagacct cggaaagaaa acaaattcag tgctaagagg ctgcagacca cacgattggg
     7501 aaaccactta gaagacagag tgaataagtt tttgcggcgc cagaatcacc ctgaagctgg
     7561 ggaggttttt gtcagagtgg tggccagctc agacaagact gtggaggtca agccgggaat
     7621 gaagtcaagg tttgtggatt ctggagagat gtcggaatct ttcccatatc gtaccaaagc
     7681 actctttgct tttgaggaga tcgatggagt cgatgtgtgc ttttttggga tgcatgtgca
     7741 agaatacggc tctgattgcc ccccaccaaa tacaaggcgt gtatacatat cttatctgga
     7801 cagtattcat ttcttccggc cccgctgcct ccggacagct gtttaccatg agatcctcat
     7861 cggatatctc gagtatgtga agaaattggg gtatgtgaca ggacatattt gggcctgtcc
     7921 cccaagtgaa ggagatgact atatctttca ttgccacccc cctgaccaga aaatccccaa
     7981 accaaaacga ctacaggagt ggtacaagaa gatgctggac aaggcgtttg cagagaggat
     8041 cattaacgac tataaggaca tcttcaaaca agcgaacgaa gacaggctca cgagtgccaa
     8101 ggagttgccc tattttgaag gagatttctg gcctaatgtg ttggaagaaa gcattaagga
     8161 actagaacaa gaagaagaag aaaggaaaaa agaagagagt actgcagcga gtgagactcc
     8221 tgagggcagt cagggtgaca gcaaaaatgc gaagaaaaag aacaacaaga agaccaacaa
     8281 aaacaaaagc agcattagcc gcgccaacaa gaagaagccc agcatgccca atgtttccaa
     8341 cgacctgtcg cagaagctgt atgccaccat ggagaagcac aaggaggtat tctttgtgat
     8401 tcatctgcat gctgggcctg ttatcagcac tcagcccccc atcgtggacc ctgatcctct
     8461 gcttagctgt gacctcatgg atgggcgaga tgccttcctc accctggcca gagacaagca
     8521 ctgggaattc tcttccttac gccgctccaa atggtccact ctgtgcatgc tggtggagct
     8581 gcacacacag ggccaggacc gctttgttta tacctgcaat gagtgcaaac accatgtgga
     8641 aacacgctgg cactgcactg tgtgtgagga ctatgacctt tgtatcaatt gctacaacac
     8701 aaagagccac acccataaga tggtgaagtg ggggctaggc ctagatgatg agggcagcag
     8761 tcagggtgag ccacagtcca agagccccca ggaatcccgg cgtctcagca tccagcgctg
     8821 catccagtcc ctggtgcatg cctgccagtg tcgcaatgcc aactgctcac tgccgtcttg
     8881 ccagaagatg aagcgagtcg tgcagcacac caagggctgc aagcgcaaga ctaatggagg
     8941 atgcccagtg tgcaagcagc tcattgctct ttgctgctac cacgccaaac actgccaaga
     9001 aaataaatgc cctgtgccct tctgcctcaa catcaaacat aagctccgcc agcagcagat
     9061 ccagcatcgc ctgcagcagg ctcagctcat gcgccggcga atggcaacca tgaacacccg
     9121 caatgtgcct cagcagagtt tgccttctcc tacctcagca ccacccggga ctcctacaca
     9181 gcagcccagc acaccccaaa caccacagcc cccagcccag cctcagcctt cacctgttaa
     9241 catgtcacca gctggcttcc ctaatgtagc ccggactcag cccccaacaa tagtgtctgc
     9301 tgggaagcct accaaccagg tgccagctcc cccaccccct gcccagcccc cacctgcagc
     9361 agtagaagca gcccggcaaa ttgaacgtga ggcccagcag cagcagcacc tataccgagc
     9421 aaacatcaac aatggcatgc ccccaggacg tgcaggtatg gggaccccag gaagccaaat
     9481 gactcctgtg ggcctgaatg tgccccgtcc caaccaagtc agtgggcctg tcatgtctag
     9541 tatgccacct gggcagtggc agcaggcacc catccctcag cagcagccga tgccaggcat
     9601 gcccaggcct gtaatgtcca tgcaggccca ggcagcagtg gctgggccac ggatgcccaa
     9661 tgtgcagcca ccaaggagca tctcgccaag tgccctgcaa gacctgctac ggaccctaaa
     9721 gtcacccagc tctcctcagc agcagcagca ggtgctgaac atccttaaat caaacccaca
     9781 gctaatggca gctttcatca aacagcgcac agccaagtat gtggccaatc agcctggcat
     9841 gcagccccag cccggacttc aatcccagcc tggtatgcag ccccagcctg gcatgcacca
     9901 gcagcctagt ttgcaaaacc tgaacgcaat gcaagctggt gtgccacggc ctggtgtgcc
     9961 tccaccacaa ccagcaatgg gaggcctgaa tccccaggga caagctctga acatcatgaa
    10021 cccaggacac aaccccaaca tgacaaacat gaatccacag taccgagaaa tggtgaggag
    10081 acagctgcta cagcaccagc agcagcagca gcaacagcag cagcagcagc agcaacaaca
    10141 aaatagtgcc agcttggccg ggggcatggc gggacacagc cagttccagc agccacaagg
    10201 acctggaggt tatgccccag ccatgcagca gcaacgcatg caacagcacc tccccatcca
    10261 gggcagctcc atgggccaga tggctgctcc aatgggacaa cttggccaga tggggcagcc
    10321 tgggctaggg gcagacagca cccctaatat ccagcaggcc ctgcagcaac ggattctgca
    10381 gcagcagcag atgaagcaac aaattgggtc accaggccag ccgaacccca tgagccccca
    10441 gcagcacatg ctctcaggac agccacaggc ctcacatctc cctggccagc agatcgccac
    10501 atcccttagt aaccaggtgc gatctccagc ccctgtgcag tctccacggc cccaatccca
    10561 acctccacat tccagcccgt caccacggat acaaccccag ccttcaccac accatgtttc
    10621 accccagact ggttcccctc accctggact cgcagtcacc atggccagct ccatggatca
    10681 gggacacctg gggaaccctg aacagagtgc aatgctcccc cagctgaata cccccaacag
    10741 gagcgcactg tccagtgaac tgtccctggt tggtgatacc acgggagaca cactagaaaa
    10801 gtttgtggag ggtttgaagc tggatccggg gatggcctac ccatacgacg tgcctgacta
    10861 cgcctcctag cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
    10921 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    10981 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    11041 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    11101 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    11161 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
    11221 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    11281 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    11341 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    11401 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    11461 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    11521 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    11581 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    11641 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    11701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    11761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    11821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    11881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
    11941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
    12001 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
    12061 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    12121 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    12181 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    12241 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    12301 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    12361 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    12421 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    12481 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    12541 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    12601 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    12661 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    12721 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    12781 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    12841 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    12901 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    12961 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    13021 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    13081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13141 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    13201 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    13261 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    13321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    13381 cgcgcgggga gaggcggttt gcgtattggg cgct
//
