LOCUS       sequence-237965-       16248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-fluorescence reporter to monitor the expression of HIV-1 early 
            genes and late genes simultaneously..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Xiao H, Wyler E, Milek M, Grewe B, Kirchner P, Ekici A, Silva ABOV, 
            Jungnickl D, Full F, Thomas M, Landthaler M, Ensser A, Uberla K
  TITLE     CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and
            Cellular mRNAs.
  JOURNAL   mBio. 2021 Jan 19;12(1). pii: mBio.02525-20. doi: 
            10.1128/mBio.02525-20.
  PUBMED    33468685
REFERENCE   2  (bases 1 to 16248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-01-19"; volume: "12(1). pii: mBio.02525-20. doi"; pages:
            " 10.1128/mBio.02525-20"
COMMENT     Sequence Label: HIV-dual-GT
FEATURES             Location/Qualifiers
     source          1..16248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             78..258
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             414..1913
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1920..2627
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     misc_feature    5248..5365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8221..8454
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8639..8683
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8977..9003
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9267..9941
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFMYGSKVYTKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERTEARHHL
                     FQ"
     primer_bind     9662..9681
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9856..9879
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             10134..10767
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12407..12424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12567..13155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12647..12666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13326..14117,14118..14186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13949..13968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14187..14291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14359..14377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14415..14437)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14537..14556
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtgtggc ctgggcggga ctggggagtg gcgagccctc agatgctaca tataagcagc
       61 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg ctcaaagtag
      181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      241 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag taaagccaga
      301 ggagatctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      361 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      421 cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt cggttaaggc
      481 cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg gagctagaac
      541 gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa atactgggac
      601 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacaatag
      661 cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa gccttagata
      721 agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca gctgacacag
      781 gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag gggcaaatgg
      841 tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg
      901 ctttcagccc agaagtaata cccatgtttt cagcattatc agaaggagcc accccacaag
      961 atttaaatac catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag
     1021 agaccatcaa tgaggaagct gcagaatggg atagattgca tccagtgcat gcagggccta
     1081 ttgcaccagg ccagatgaga gaaccaaggg gaagtgacat agcaggaact actagtaccc
     1141 ttcaggaaca aataggatgg atgacacata atccacctat cccagtagga gaaatctata
     1201 aaagatggat aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc
     1261 tggacataag acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa
     1321 ctctaagagc cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg
     1381 tccaaaatgc gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac
     1441 tagaagaaat gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt
     1501 tggctgaagc aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt
     1561 ttaggaacca aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca
     1621 aaaattgcag ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa
     1681 tgaaagattg tactgagaga caggctaact tcctggggaa gatctggcct tcccacaagg
     1741 gaaggccagg gaattttctt cagagcagac cagagccaac agccccacca gaagagagct
     1801 tcaggtttgg ggaagagaca acaactccct ctcagaagca ggagccgata gacaaggaac
     1861 tgtatccttt agcttccctc agatcactct ttggcagcga cccctcgtca caagctagcg
     1921 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     1981 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     2041 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     2101 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     2161 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     2221 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     2281 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     2341 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     2401 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat
     2461 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     2521 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     2581 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatagc catggcttcc
     2641 cgccggcggt ggcggcgcag gatgatggca cgctgcccat gtcttgtgcc caggagagcg
     2701 ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtaa tagtctagaa
     2761 gatagggggg caattaaagg aagctctatt agatacagga gcagatgata cagtattaga
     2821 agaaatgaat ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat
     2881 caaagtaaga cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac
     2941 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
     3001 ctgcacttta aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg
     3061 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
     3121 agaaatttgt acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc
     3181 atacaatact ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt
     3241 agatttcaga gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc
     3301 acatcctgca gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata
     3361 tttttcagtt cccttagata aagacttcag gaagtatact gcatttacca tacctagtat
     3421 aaacaatgag acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg
     3481 atcaccagca atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa
     3541 tccagacata gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat
     3601 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac
     3661 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
     3721 tcctgataaa tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa
     3781 tgacatacag aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa
     3841 agtaaggcaa ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact
     3901 aacagaagaa gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca
     3961 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg
     4021 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc
     4081 aagaatgaag ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat
     4141 agccacagaa agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa
     4201 ggaaacatgg gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga
     4261 gtttgtcaat acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat
     4321 aggagcagaa actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc
     4381 aggatatgta actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca
     4441 gaagactgag ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat
     4501 agtgacagac tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc
     4561 agagttagtc agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg
     4621 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg
     4681 aatcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
     4741 tcacagtaat tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga
     4801 aatagtagcc agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga
     4861 ctgtagccca ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt
     4921 agcagttcat gtagccagtg gatatataga agcagaagta attccagcag agacagggca
     4981 agaaacagca tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac
     5041 agacaatggc agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat
     5101 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa
     5161 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc
     5221 agtacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     5281 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     5341 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     5401 gaaaggacca gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag
     5461 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat
     5521 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat taacacatgg aaaagattag
     5581 taaaacacca tatgtagatt tcaaggaaag ctaaggactg gttttataga catcactatg
     5641 aaagtactaa tccaaaaata agttcagaag tacacatccc actaggggat gctaaattag
     5701 taataacaac atattggggt ctgcatacag gagaaagaga ctggcatttg ggtcagggag
     5761 tctccataga atggaggaaa aagagatata gcacacaagt agaccctgac ctagcagacc
     5821 aactaattca tctgcactat tttgattgtt tttcagaatc tgctataaga aataccatat
     5881 taggacgtat agttagtcct aggtgtgaat atcaagcagg acataacaag gtaggatctc
     5941 tacagtactt ggcactagca gcattaataa aaccaaaaca gataaagcca cctttgccta
     6001 gtgttaggaa actgacagag gacagatgga acaagcccca gaagactaag ggccacagag
     6061 ggagccatac aatgaatgga cactagagct tttagaggaa cttaagagtg aagctgttag
     6121 acattttcct aggatatggc tccataactt aggacaacat atctatgaaa cttacgggga
     6181 tacttgggca ggagtggaag ccataataag aattctgcaa caactgctgt ttatccattt
     6241 cagaattggg tgtcgacata gcagaatagg cgttactcga cagaggagag caagaaatgg
     6301 agccagtaga tcctagacta gagccctgga agcatccagg aagtcagcct aaaactgctt
     6361 gtaccaattg ctattgtaaa aagtgttgct ttcattgcca agtttgtttc atgacaaaag
     6421 ccttaggcat ctcctacggc aggaagaagc ggagacagcg acgaagagct catcagaaca
     6481 gtcagactca tcaagcttct ctaacaaagc agtaagtagt acatgtaatg caaccttaat
     6541 agtagcaata gtagcattag tagtagcaat aataatagca atagttgtgt ggtccatagt
     6601 aatcatagaa tataggaaaa tattaagaca aagaaaaata gacaggttaa ttgatagact
     6661 aatagaaaga gcagaagaca gtggcaatga gagtgaagga gaagtatcag cacttgtgga
     6721 gatgggggtg gaaatggggc accatgcgcc gtgggacatc gatgatctgt agtgctacag
     6781 aaaaattgtg ggtcacagtc tattatgggg tacctgtgtg gaaggaagca accaccactc
     6841 tattttgtgc atcagatgct aaagcatatg atacagaggt acataatgtt tgggccacac
     6901 atgcctgtgt acccacagac cccaacccac aagaagtagt attggtaaat gtgacagaaa
     6961 attttaacat gtggaaaaat gacatggtag aacagatgca tgaggatata atcagtttat
     7021 gggatcaaag cctaaagcca tgtgtaaaat taaccccact ctgtgttagt ttaaagtgca
     7081 ctgatttgaa gaatgatact aataccaata gtagtagcgg gagaatgata atggagaaag
     7141 gagagataaa aaactgctct ttcaatatca gcacaagcat aagagataag gtgcagaaag
     7201 aatatgcatt cttttataaa cttgatatag taccaataga taataccagc tataggttga
     7261 taagttgtaa cacctcagtc attacacagg cctgtccaaa ggtatccttt gagccaattc
     7321 ccatacatta ttgtgccccg gctggttttg cgattctaaa atgtaataat aagacgttca
     7381 atggaacagg accatgtaca aatgtcagca cagtacaatg tacacatgga atcaggccag
     7441 tagtatcaac tcaactgctg ttaaatggca gtctagcaga agaagatgta gtaattagat
     7501 ctgccaattt cacagacaat gctaaaacca taatagtaca gctgaacaca tctgtagaaa
     7561 ttaattgtac aagacccaac aacaatacaa gaaaaagtat ccgtatccag aggggaccag
     7621 ggagagcatt tgttacaata ggaaaaatag gaaatatgag acaagcacat tgtaacatta
     7681 gtagagcaaa atggaatgcc actttaaaac agatagcaaa ttaagagaac aatttggaaa
     7741 taataaaaca ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag
     7801 ttttaattgt ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg
     7861 gtttaatagt acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac
     7921 actcccatgc agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta
     7981 tgcccctccc atcagcggac aaattagatg ttcatcaaat attacagggc tgctattaac
     8041 aagagatggt ggtaataaca acaatgggtc cgagatcttc agacctggag gaggagatat
     8101 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8161 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8221 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8281 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8341 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8401 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8461 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8521 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8581 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8641 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8701 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8761 aagaatagtt tttgctgtac tttctgtagt gaatagagtt aggcagggat attcaccatt
     8821 atcgtttcag acccacctcc caatcccgag gggacccgac aggcccgaag gaatagaaga
     8881 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ccttagcact
     8941 tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact
     9001 cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata
     9061 ttggtggaat ctcctacagt attggagtca ggaactaaag aatagtgctg ttaacttgct
     9121 caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag tattacaagc
     9181 agcttataga gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt
     9241 gctataagat gggtggcgcg gccgctgata gcactgagaa cgtcatcaag cccttcatgc
     9301 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     9361 gcgagggcaa gccctacgag ggcacccaga ccgccaagct gcaggtgacc aagggcggcc
     9421 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggtgtaca
     9481 ccaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt
     9541 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     9601 tgcaggacgg caccttcatc taccacgtga agttcatcgg cgtgaacttc ccctccgacg
     9661 gccccgtaat gcagaagaag actctgggct gggagccctc caccgagcgc ctgtaccccc
     9721 gcgacggcgt gctgaagggc gagatccaca aggcgctgaa gctgaagggc ggcggccact
     9781 acctgtgtga gttcaagtca atctacatgg ccaagaagcc cgtgaagctg cccggctact
     9841 actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc gtggtggagc
     9901 agtacgagcg caccgaggcc cgccaccacc tgttccagta gtaactcgag acctagaaaa
     9961 acatggagca atcacaagta gcaatacagc agctaacaat gctgcttgtg cctggctaga
    10021 agcacaagag gaggaagagg tgggttttcc agtcacacct caggtacctt taagaccaat
    10081 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10141 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
    10201 ctacttccct gattggcaga actacacacc agggccaggg gtcagatatc cactgacctt
    10261 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
    10321 agagaacacc agcttgttac accctgtgag cctgcatgga atggatgacc ctgagagaga
    10381 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
    10441 tccggagtac ttcaagaact gctgacatcg agcttgctac aagggacttt ccgctgggga
    10501 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatgctgcat
    10561 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10741 tttagtcagt gtggaaaatc tctagcaccc aggaggtaga ggttgcagtg agccaagatc
    10801 gcgccactgc attccagcct gggcaagaaa acaagactgt ctaaaataat aataataagt
    10861 taagggtatt aaatatattt atacatggag gtcataaaaa tatatatatt tgggctgggc
    10921 gcagtggctc acacctgcgc ccggcccttt gggaggccga ggcaggtgga tcacctgagt
    10981 ttgggagttc cagaccagcc tgaccaacat ggagaaaccc cttctctgtg tatttttagt
    11041 agattttatt ttatgtgtat tttattcaca ggtatttctg gaaaactgaa actgtttttc
    11101 ctctactctg ataccacaag aatcatcagc acagaggaag acttctgtga tcaaatgtgg
    11161 tgggagaggg aggttttcac cagcacatga gcagtcagtt ctgccgcaga ctcggcgggt
    11221 gtccttcggt tcagttccaa caccgcctgc ctggagagag gtcagaccac agggtgaggg
    11281 ctcagtcccc aagacataaa cacccaagac ataaacaccc aacaggtcca ccccgcctgc
    11341 tgcccaggca gagccgattc accaagacgg gaattaggat agagaaagag taagtcacac
    11401 agagccggct gtgcgggaga acggagttct attatgactc aaatcagtct ccccaagcat
    11461 tcggggatca gagtttttaa ggataactta gtgtgtaggg ggccagtgag ttggagatga
    11521 aagcgtaggg agtcgaaggt gtccttttgc gccgagtcag ttcctgggtg ggggccacaa
    11581 gatcggatga gccagtttat caatccgggg gtgccagctg atccatggag tgcagggtct
    11641 gcaaaatatc tcaagcactg attgatctta ggttttacaa tagtgatgtt accccaggaa
    11701 caatttgggg aaggtcagaa tcttgtagcc tgtagctgca tgactcctaa accataattt
    11761 cttttttgtt tttttttttt tatttttgag acagggtctc actctgtcac ctaggctgga
    11821 gtgcagtggt gcaatcacag ctcactgcag cctcaacgtc gtaagctcaa gcgatcctcc
    11881 cacctcagcc tgcctggtag ctgagactac aagcgacgcc ccagttaatt tttgtatttt
    11941 tggtagaggc agcgttttgc cgtgtggccc tggctggtct cgaactcctg ggctcaagtg
    12001 atccagcctc agcctcccaa agtgctggga caaccggggc cagtcactgc acctggccct
    12061 aaaccataat ttctaatctt ttggctaatt tgttagtcct acaaaggcag tctagtcccc
    12121 aggcaaaaag ggggtttgtt tcgggaaagg gctgttactg tctttgtttc aaactataaa
    12181 ctaagttcct cctaaactta gttcggccta cacccaggaa tgaacaagga gagcttggag
    12241 gttagaagca cgatggaatt ggttaggtca gatctctttc actgtctgag ttataatttt
    12301 gcaatggtgg ttcaaagact gcccgcttct gacaccagtc gctgcattaa tgaatcggcc
    12361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    12421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac
    12541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    12601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    12661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    12721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    12781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    12901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    12961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    13021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    13081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    13141 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    13201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    13261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13381 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    13441 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    13501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    13561 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    13621 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    13681 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    13741 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    13801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13861 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    13921 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    13981 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    14041 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    14101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    14161 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    14221 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    14281 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    14341 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    14401 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    14461 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    14521 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    14581 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    14641 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccaggggagg
    14701 cagagattgc agtaagctga gatcgcagca ctgcactcca gcctgggcga cagagtaaga
    14761 ctctgtctca aaaataaaat aaataaatca atcagatatt ccaatctttt cctttattta
    14821 tttatttatt ttctattttg gaaacacagt ccttccttat tccagaatta cacatatatt
    14881 ctatttttct ttatatgctc cagttttttt tagaccttca cctgaaatgt gtgtatacaa
    14941 aatctaggcc agtccagcag agcctaaagg taaaaaataa aataataaaa aataaataaa
    15001 atctagctca ctccttcaca tcaaaatgga gatacagctg ttagcattaa ataccaaata
    15061 acccatcttg tcctcaataa ttttaagcgc ctctctccac cacatctaac tcctgtcaaa
    15121 ggcatgtgcc ccttccgggc gctctgctgt gctgccaacc aactggcatg tggactctgc
    15181 agggtcccta actgccaagc cccacagtgt gccctgaggc tgccccttcc ttctagcggc
    15241 tgcccccact cggctttgct ttccctagtt tcagttactt gcgttcagcc aaggtctgaa
    15301 actaggtgcg cacagagcgg taagactgcg agagaaagag accagcttta cagggggttt
    15361 atcacagtgc accctgacag tcgtcagcct cacagggggt ttatcacatt gcaccctgac
    15421 agtcgtcagc ctcacagggg gtttatcaca gtgcaccctt acaatcattc catttgattc
    15481 acaatttttt tagtctctac tgtgcctaac ttgtaagtta aatttgatca gaggtgtgtt
    15541 cccagagggg aaaacagtat atacagggtt cagtactatc gcatttcagg cctccacctg
    15601 ggtcttggaa tgtgtccccc gaggggtgat gactacctca gttggatctc cacaggtcac
    15661 agtgacacaa gataaccaag acacctccca aggctaccac aatgggccgc cctccacgtg
    15721 cacatggccg gaggaactgc catgtcggag gtgcaagcac acctgcgcat cagagtcctt
    15781 ggtgtggagg gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg
    15841 aaagccccag ttctacttac accaggaaag gctggaaggg ctaatttggt cccaaaaaag
    15901 acaagagatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
    15961 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct tcaagttagt
    16021 accagttgaa ccagagcaag tagaagaggc caatgaagga gagaacaaca gcttgttaca
    16081 ccctatgagc cagcatggga tggaggaccc ggagggagaa gtattagtgt ggaagtttga
    16141 cagcctccta gcatttcgtc acatggcccg agagctgcat ccggagtact acaaagactg
    16201 ctgacatcga gctttctaca agggactttc cgctggggac tttccagg
//
