LOCUS       sequence-237966-        6240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF4
FEATURES             Location/Qualifiers
     source          1..6240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3364..3463)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3480..3499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3481..3499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3504..3520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3504..3520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3951..4742
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4835..5423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5324..5343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5577..5594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5753..5780)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5872..5958)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6007..6029
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6021..6038
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6022..6038
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6054..6153
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcatcactaa agctgctatt aaagctatta agctttataa gcatgtagtt caaatagtag
       61 aaaagttcat caaaaagtgt aaaccagaat acaaggttcc gggattatat gtaattgact
      121 caattgtgcg acagtctcgt catcagtttg gaactgataa agatgttttt gggccaagat
      181 tctctaaaaa cataactgcc acattccaat atttatatct ttgtccatct gaagataaga
      241 gtaaaatagt tcgtgtgctg aacctttggc aaaaaaatgg agtgttcaaa attgaaatta
      301 ttcaacctct tttggacatg gcagcgggaa ccagtaatgc agccccagta gcagaaaatg
      361 ttaccaataa tgaaggctca cctccacctc cagtaaaagt ttcttctgaa cctcccacac
      421 aagccactcc aaactccgtc ccagctgtac cacagttgcc cagctctgat gcttttgctg
      481 ctgtggctca gctgtttcag acaactcaag gccaacagct tcagcagatc cttcagactt
      541 ttcaacagcc tccaaaacca cagtctcctg cccttgacaa tgctgtgatg gctcaggttc
      601 aggctatcac agctcagtta aagacaactc ctacacaacc atctgaacaa aaagctgctt
      661 tccccccacc tgaacagaaa actgcatttg acaagaagtt gcttgataga tttgactatg
      721 atgatgagcc agaagctgtg gaagaatcaa agaaagagga taccactgcc gtcaccacga
      781 cagcacctgc tgccgcagta ccccctgcac ccaccgccac cgtgcctgct gctgctgcac
      841 ccgctgctgc ctctcctcct cctccacagg caccatttgg ctttcctgga gatggcatgc
      901 agcagccagc atacacacag catcaaaata tggatcagtt tcagccacga atgatgggaa
      961 tacaacagga tccaatgcac catcaggttc cacttcctcc taatggacaa atgccaggat
     1021 ttggacttct tcctacacct ccatttcctc ccatggctca gcctgtgatt cctccaactc
     1081 caccagtgca gcagcctttc caagcttctt ttcaggcaca aaatgaacca cttacacaga
     1141 agccgcatca gcaggaaatg gaagtagaac aaccttgtat tcaagaggtt aagcgacata
     1201 tgtctgataa cagaaagtca agatctaggt cagcatccag gtcaccaaaa aggaggcgat
     1261 ctagatctgg ttctagatct cgaaggtctc ggcatcgacg ttctcgatct cggtccaggg
     1321 atagacgccg acattctccc cgatctcgat ctcaagaaag acgggatcga gaaaaagaga
     1381 gagaacgtcg acaaaaaggc ctccctcaag tgaaaccgga aactgcaagt gtttgcagta
     1441 ctaccctctg ggtggggcag ctggacaaaa gaactactca gcaggatgtt gccagtctct
     1501 tggaagagtt tggtccaatt gaatcaatta atatgattcc tcccaggggt tgtgcctata
     1561 ttgttatggt tcataggcaa gatgcctatc gtgccctgca gaaactgagc cgaggaaact
     1621 ataaagtgaa ccagaaatcc ataaagattg cctgggcctt aaacaaagga ataaaggcag
     1681 attataagca gtattgggat gtagaacttg gtgttactta tattccatgg gacaaagtca
     1741 agcctgagga actggagagt ttttgtgaag gaggaatgtt ggacagtgac acacttaacc
     1801 cagattggaa aggaattcct aagaagcctg aaaatgaagt tgctcaaaat ggaggtgctg
     1861 aaacctcaca cacagaacca gtatcaccca tacctaaacc attacctgtg cctgtccctc
     1921 ctattcctgt tcctgcacct ataacagtgc cacctccaca ggtcccacca catcaaccgg
     1981 gtccacctgt agttggtgct ctccagccgc ctgctttcac gcctcctctg ggaataccgc
     2041 ctccaggctt tggtcctggt gttcctcctc cccctcctcc tccaccattt ttgcgcccag
     2101 gattcaaccc aatgcattta ccaccaggtt ttctgcctcc tggaccccca cctcctataa
     2161 ctccaccagt atccattcct cctcctcaca ctccaccaat aagcatccca aactctacta
     2221 tcgctggtat aaatgaagac actacaaaag acttatctat tggaaatccc attccaacag
     2281 tggtgtctgg ggctagagga aacgccgagt ctggtgacag cgtgaaaatg tatggctctg
     2341 ccgtgccacc tgctgcaccc acgaatctgc ccacccctcc tgtaacccag cctgtttcac
     2401 ttcttggcac tcaaggagtt gcccctggtc ctgtaattgg acttcaggca ccatctactg
     2461 gtcttcttgg cgcccggccc ggtctcatcc cactccagcg ccctccagga atgcccccac
     2521 ctcacttaca gcggttccct ttgatgccgc cccgtcccat gccaccgcac atgatgcaca
     2581 gaggcccacc gccaggacca gggggctttg cgatgcctcc acctcatgga atgaaaggtc
     2641 ccttcccacc gcatggcccc tttgttaggc ctggtggaat gccagggctc gggggcccag
     2701 ggccaggccc agggggtcct gaagacagag acggaaggca acagccgccg cagcagccac
     2761 agcagcagcc acagccgcag gcgccccagc aaccacagca gcagcagcag cagcagccac
     2821 caccatcaca acagcctcca ccaacacagc agcagccaca gcagtttaga aatgataaca
     2881 ggcagcagtt caattcaggt agagaccaag aaaggtttgg aagaagatct tttggaaata
     2941 gggtggaaaa tgaccgggaa cggtatggga accgtaatga tgatagagat aatagtaacc
     3001 gtgacaggag agagtgggga aggaggagcc ctgaccggga caggcacaga gacttggaag
     3061 agagaaatag acgctctagt gggcatcgag acagagagag agattctaga gatagagagt
     3121 ctcgtagaga gaaggaagaa gcccgaggaa aggaaaagcc tgaggtgaca gacagggcag
     3181 gtggtaacaa aaccgttgaa cctcccatta gccaagtggg aaatgtagac actgcttcag
     3241 aacttgagaa gggggtgtct gaggctgcag tcctaaagcc ttctgaagag ttacctgctg
     3301 aggctacctc atccgttgaa cccgaaaagg attctggctc agcagcagag gctcctcgtt
     3361 aaaacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     3421 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     3481 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3541 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     3601 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     3661 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3721 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3781 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3841 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3901 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3961 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     4021 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     4081 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     4141 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     4201 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     4261 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     4321 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     4381 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     4441 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     4501 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     4561 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     4621 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     4681 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4741 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4981 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5461 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5521 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5581 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5641 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5701 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     5761 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5821 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5881 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5941 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     6001 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     6061 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     6121 ttataatgcc aactttgtac aaaaaagcag gcttcaccat ggacgccgtc aacgccttca
     6181 accaggagct cttttcgctt atggatatga aacctcccat ctctagagcc aagatgattc
//
