LOCUS       sequence-237967-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_br48_g4458
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(336..352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(336..352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(349..371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    360..376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(384..390,391..408,409..414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    429..450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(567..584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(818..837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atggttgatc caccagcttc tccaggtggt tctttgcaaa ttccaccaac
       61 ttctaatgtt ttgtgtccaa ttggtattaa atgtgctgct tcttctgatg gtactcatag
      121 atgtttgggt ttgaatggta gagctatttg tgctactact tgtccaacta cttctgattg
      181 tccagctaga tgtaaaaaac aaactttggt taatggtttt tgtactaatg gtgctaatcc
      241 atgtatttgt actaatactg atgctattat tagacaatct tgagcttaga gaccaagttc
      301 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      901 tttctccctt cgggaagcgt g
//
