LOCUS       sequence-237971-        6240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF4_CRISPR resistant
FEATURES             Location/Qualifiers
     source          1..6240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     136..158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     150..167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     151..167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    183..282
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3733..3832)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3849..3868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3850..3868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3873..3889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3873..3889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4320..5111
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5204..5792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5693..5712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5946..5963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6122..6149)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      121 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      181 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      241 aatgcttttt tataatgcca actttgtaca aaaaagcagg cttcaccatg gacgccgtca
      301 acgccttcaa ccaggagctc ttttcgctta tggatatgaa acctcccatc tctagagcca
      361 agatgattct catcactaaa gctgctatta aagctattaa gctttataag catgtagttc
      421 aaatagtaga aaagttcatc aaaaagtgta aaccagaata caaggttccg ggattatatg
      481 taattgactc aattgtgcga cagtctcgtc atcagtttgg aactgataaa gatgtttttg
      541 ggccaagatt ctctaaaaac ataactgcca cattccaata tttatatctt tgtccatctg
      601 aagataagag taaaatagtt cgtgtgctga acctttggca aaaaaatgga gtgttcaaaa
      661 ttgaaattat tcaacctctt ttggacatgg cagcgggaac cagtaatgca gccccagtag
      721 cagaaaatgt taccaataat gaaggctcac ctccacctcc agtaaaagtt tcttctgaac
      781 ctcccacaca agccactcca aactccgtgc ctgcagttcc tcaattaccc agctctgatg
      841 cttttgctgc tgtggctcaa ctcttccaaa ctacacaggg ccaacagctt cagcagatcc
      901 ttcagacttt tcaacagcct ccaaaaccac agtctcctgc ccttgacaat gctgtgatgg
      961 ctcaggttca ggctatcaca gctcagttaa agacaactcc tacacaacca tctgaacaaa
     1021 aagctgcttt ccccccacct gaacagaaaa ctgcatttga caagaagttg cttgatagat
     1081 ttgactatga tgatgagcca gaagctgtgg aagaatcaaa gaaagaggat accactgccg
     1141 tcaccacgac agcacctgct gccgcagtac cccctgcacc caccgccacc gtgcctgctg
     1201 ctgctgcacc cgctgctgcc tctcctcctc ctccacaggc accatttggc tttcctggag
     1261 atggcatgca gcagcctgct tatacgcaac accagaatat ggatcaattc caacctcgta
     1321 tgatgggaat acaacaggat ccaatgcacc atcaggttcc acttcctcct aatggacaaa
     1381 tgccaggatt tggacttctt cctacacctc catttcctcc catggctcag cctgtgattc
     1441 ctccaactcc accagtgcag cagcctttcc aagcttcttt tcaggcacaa aatgaaccac
     1501 ttacacagaa gccgcatcag caggaaatgg aagtagaaca accttgtatt caagaggtta
     1561 agcgacatat gtctgataac agaaagtcaa gatctaggtc agcatccagg tcaccaaaaa
     1621 ggaggcgatc tagatctggt tctagatctc gaaggtctcg gcatcgacgt tctcgatctc
     1681 ggtccaggga tagacgccga cattctcccc gatctcgatc tcaagaaaga cgggatcgag
     1741 aaaaagagag agaacgtcga caaaaaggcc tccctcaagt gaaaccggaa actgcaagtg
     1801 tttgcagtac taccctctgg gtggggcagc tggacaaaag aactactcag caggatgttg
     1861 ccagtctctt ggaagagttt ggtccaattg aatcaattaa tatgattcct cccaggggtt
     1921 gtgcctatat tgttatggtt cataggcaag atgcctatcg tgccctgcag aaactgagcc
     1981 gaggaaacta taaagtgaac cagaaatcca taaagattgc ctgggcctta aacaaaggaa
     2041 taaaggcaga ttataagcag tattgggatg tagaacttgg tgttacttat attccatggg
     2101 acaaagtcaa gcctgaggaa ctggagagtt tttgtgaagg aggaatgttg gacagtgaca
     2161 cacttaaccc agattggaaa ggaattccta agaagcctga aaatgaagtt gctcaaaatg
     2221 gaggtgctga aacctcacac acagaaccag tatcacccat acctaaacca ttacctgtgc
     2281 ctgtccctcc tattcctgtt cctgcaccta taacagtgcc acctccacag gtcccaccac
     2341 atcaaccggg tccacctgta gttggtgctc tccagccgcc tgctttcacg cctcctctgg
     2401 gaataccgcc tccaggcttt ggtcctggtg ttcctcctcc ccctcctcct ccaccatttt
     2461 tgcgcccagg attcaaccca atgcatttac caccaggttt tctgcctcct ggacccccac
     2521 ctcctataac tccaccagta tccattcctc ctcctcacac tccaccaata agcatcccaa
     2581 actctactat cgctggtata aatgaagaca ctacaaaaga cttatctatt ggaaatccca
     2641 ttccaacagt ggtgtctggg gctagaggaa acgccgagtc tggtgacagc gtgaaaatgt
     2701 atggctctgc cgtgccacct gctgcaccca cgaatctgcc cacccctcct gtaacccagc
     2761 ctgtttcact tcttggcact caaggagttg cccctggtcc tgtaattgga cttcaggcac
     2821 catctactgg tcttcttggc gcccggcccg gtctcatccc actccagcgc cctccaggaa
     2881 tgcccccacc tcacttacag cggttccctt tgatgccgcc ccgtcccatg ccaccgcaca
     2941 tgatgcacag aggcccaccg ccaggaccag ggggctttgc gatgcctcca cctcatggaa
     3001 tgaaaggtcc cttcccaccg catggcccct ttgttaggcc tggtggaatg ccagggctcg
     3061 ggggcccagg gccaggccca gggggtcctg aagacagaga cggaaggcaa cagccgccgc
     3121 agcagccaca gcagcagcca cagccgcagg cgccccagca accacagcag cagcagcagc
     3181 agcagccacc accatcacaa cagcctccac caacacagca gcagccacag cagtttagaa
     3241 atgataacag gcagcagttc aattcaggta gagaccaaga aaggtttgga agaagatctt
     3301 ttggaaatag ggtggaaaat gaccgggaac ggtatgggaa ccgtaatgat gatagagata
     3361 atagtaaccg tgacaggaga gagtggggaa ggaggagccc tgaccgggac aggcacagag
     3421 acttggaaga gagaaataga cgctctagtg ggcatcgaga cagagagaga gattctagag
     3481 atagagagtc tcgtagagag aaggaagaag cccgaggaaa ggaaaagcct gaggtgacag
     3541 acagggcagg tggtaacaaa accgttgaac ctcccattag ccaagtggga aatgtagaca
     3601 ctgcttcaga acttgagaag ggggtgtctg aggctgcagt cctaaagcct tctgaagagt
     3661 tacctgctga ggctacctca tccgttgaac ccgaaaagga ttctggctca gcagcagagg
     3721 ctcctcgtta aaacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     3781 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     3841 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     3901 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
     3961 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     4021 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     4081 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     4141 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     4201 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     4261 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     4321 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     4381 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     4441 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     4501 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     4561 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     4621 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     4681 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     4741 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     4801 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     4861 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     4921 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     4981 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     5041 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     5101 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     5161 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5221 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5281 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5341 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5401 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5461 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5521 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5581 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5641 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5701 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5761 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5821 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5881 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5941 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6001 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6061 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     6121 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     6181 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
//
